Selection of yeast cells with a higher plasmid copy number in a Saccharomyces cerevisiae autoselection system

Yeast

Volumn 12, Issue 3, 1996, Pages 199-205

  Compagno, Concetta ^a   Porro, Danilo ^a   Radice, Stefania ^a   Martegani, Enzo ^a   Ranzi, Bianca Maria ^a  

^a UNIVERSITY OF MILAN   (Italy)

Author keywords

Autoselection plasmid; Flow cytometry; S. cerevisiae

Indexed keywords

ARTICLE; CELL VIABILITY; CONTROLLED STUDY; FLOW CYTOMETRY; FUNGUS GROWTH; NONHUMAN; PLASMID; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; YEAST;

FLOW CYTOMETRY; PLASMIDS; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC;

EID: 0029887647     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(19960315)12:3<199::AID-YEA895>3.0.CO;2-U     Document Type: Article

References (24)

1. Alberghina, L. and Porro, D. (1993). Quantitative flow cytometry: analysis of protein distribution in budding yeast: A mini review. Yeast 9, 815-823.
2. - strains of Saccharomyces cerevisiae. Appl. Microbiol Biotechnol. 37, 615-620.
3. Broach, J. R. (1983). Construction of high copy yeast vector using 2-μm circle sequences. In Wu, R., Grossman, L. and Moldave, K. (Eds), Methods in Enzymology, vol. 101. Academic Press, Inc., New York, pp. 307-325.
4. Compagno, C., Ranzi, B. M. and Martegani, E. (1991). The promoter of Saccharomyces cerevisiae FBA1 gene contains a single positive upstream regulatory element. FEBS Lett. 293, 97-100.
5. Compagno, C., Tura, A., Ranzi, B. M., Alberghina, L. and Martegani, E. (1993). Copy number modulation in an autoselection system for stable plasmid maintenance in Saccharomyces cerevisiae. Biotechnol. Prog. 9, 594-599.
6. De la Fuente, J. M., Alvarez, A., Nombela, C. and Sanchez, M. (1992). Flow cytometric analysis of Saccharomyces cerevisiae autolytic mutants and protoplasts. Yeast 8, 39-45.
7. Eitzman, P. D. and Srienc, F. (1991). Dynamics of activation of a galactose-inducible promoter in Saccharomyces cerevisiae. J. Biotechnol. 21, 63-82.
8. Erhart, E. and Hollemberg, C. P. (1983). The presence of a defective LEU2 gene in 2 μm DNA recombinant plasmid of Saccharomyces cerevisiae is responsible for curing and high copy number. J. Bacteriol. 156, 625-635.
9. Fleer, R. (1992). Engineering yeast for high level expression. Current Opinion in Biotechnology 3, 486-496.
10. Futcher, A. B. (1988). The 2 μm circle plasmid of Saccharomyces cerevisiae. Yeast 4, 27-40.
11. Loison, G., Nguyen-Juilleret, M., Alouani, F. and Marquet, M. (1986). Plasmid-transformed URA3 FUR1 double mutant of Saccharomyces cerevisiae: an autoselection system applicable to the production of foreign proteins. Bio/Technology 4, 433-437.
12. Loison, G., Vidal, A., Findelli, A., et al. (1989). High level of expression of a protective antigen of schistosomes in Saccharomyces cerevisiae. Yeast 5, 497-507.
13. Ludwig, D. L., Ugolini, S. and Bruschi, C. V. (1993). High-level heterologous gene expression in Saccharomyces cerevisiae from a stable 2 μm plasmid system. Gene 132, 33-40.
14. Martegani, E., Brambilla, L., Porro, D., Ranzi, B. M. and Alberghina, L. (1993). Alteration of cell population structure due to cell lysis in Saccharomyces cerevisiae cells overexpressing the GAL4 gene. Yeast 9, 575-582.
15. Morrissey, J. A. and Cashmore, A. M. (1992). Use of a cis-acting mutation to study the role of FLP-mediated recombination in the maintenance of native yeast 2 μm plasmids. Mol. Microbiol. 6, 3101-3107.
16. Napp, S. J. and Da Silva, N. A. (1993). Enhancement of cloned gene product synthesis via autoselection in recombinant Saccharomyces cerevisiae. Biotechnol. Bioeng. 41, 801-810.
17. Nasmyth, K. A. and Reed, S. I. (1980). Isolation of genes by complementation in yeast, molecular cloning of a cell-cycle gene. Proc. Natl Acad. Sci. USA 77, 2119-2123.
18. Piper, P. W. and Curran, B. P. G. (1990). When a glycolytic gene on a yeast 2 μ ORI-STB plasmid is made essential for growth its expression level is a major determinant of plasmid copy number. Curr Genet. 17, 119-123.
19. Porro, D., Smeraldi, C., Martegani, E., Ranzi, B. M. and Alberghina, L. (1994). Flow-cytometric determination of the respiratory activity in growing Saccharomyces cerevisiae population. Biotechnol. Prog 10, 193-197.
20. Porro, D. and Srienc, F. (1995). Tracking of individual cell cohorts in asynchronous Saccharomyces cerevisiae population. Biotechnol. Prog. 11, 342-347.
21. Rech, S. B., Stateva, L. I. and Oliver, S. G. (1992). Complementation of the Saccharomyces cerevisiae srb1-1 mutation: an autoselection system for stable plasmid maintenance. Curr. Genet. 21, 339-344.
22. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
23. Unternahrer, S., Pridmore, D. and Hinnen, A. (1991). A new system for amplifying 2 μm plasmid copy number in Saccharomyces cerevisiae. Mol. Microbiol. 5, 1539-1548.
24. Wittrup, K. D., Bailey, J. D., Ratzkin, B. and Patel, A. (1990). Propagation of an amplifiable recombinant plasmid in Saccharomyces cerevisiae. Flow cytometry studies and segregated modeling. Biotechnol Bioeng. 35, 565-577.