Carboxylmethylation of the catalytic subunit of protein phosphatase 2A in insulin-secreting cells: Evidence for functional consequences on enzyme activity and insulin secretion

Endocrinology

Volumn 137, Issue 6, 1996, Pages 2315-2323

  Kowluru, Anjaneyulu ^b   Seavey, Scott E ^c   Rabaglia, Mary E ^c   Nesher, Rafael ^a   Metz, Stewart A ^c  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b University of Wisconsin Health   (United States)

^c NONE

Author keywords

[No Author keywords available]

Indexed keywords

EBELACTONE A; OKADAIC ACID; PHOSPHOPROTEIN PHOSPHATASE;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CARBOXYLATION; COMPETITIVE INHIBITION; DEMETHYLATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SUBUNIT; EXOCYTOSIS; INSULIN RELEASE; METHYLATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN MODIFICATION; PROTEIN PHOSPHORYLATION; RAT;

EID: 0029887332     PISSN: 00137227     EISSN: None     Source Type: Journal    
DOI: 10.1210/endo.137.6.8641181     Document Type: Article

References (45)

1. 2+, cAMP, and phospholipid-derived messengers in coupling mechanisms of insulin secretion. Physiol Rev 67:1185-1248
2. Metz SA 1986 Ether-linked lysophospholipids initiate insulin secretion. Lysophospholipids may mediate effects of phospholipase A2 activation on hormone release. Diabetes 35:808-817
3. Metz SA 1991 The pancreatic islet as Rubik's cube. Is phospholipid hydrolysis a piece of the puzzle? Diabetes 40:1565-1573
4. Sugden MC, Aschcroft SJH, Sugden PH 1979 Protein kinase activities in rat pancreatic islets of Langerhans. Biochem J 180:219-229
5. MacDonald MJ, Kowluru A 1982 Calcium-calmodulin-dependent myosin phosphorylation by pancreatic islets. Diabetes 31:566-570
6. 2+ and calmodulin-dependent protein kinase activity with insulin secretion from islets of Langerhans. Biochem J 212:819-827
7. 2+ and cAMP-enhanced phosphorylation of two 57,000 Mr proteins. Biochem Biophys Res Commun 118:797-804
8. Kowluru A, MacDonald MJ 1984 Glucose-induced, calcium-mediated protein phosphorylation in intact pancreatic islets. Arch Biochem Biophys 231:320-327
9. Persaud SJ, Jones PM, Howell SL 1990 Glucose-stimulated insulin secretion is not dependent on activation of protein kinase A. Biochem Biophys Res Commun 173:833-839
10. Basudev H, Jones PM, Persaud S, Howell SL 1993 Arachidonic acid-induced insulin secretion from rat islets of Langerhans is not mediated by protein phosphorylation. Mol Cell Endocrinol 91:193-199
11. Cohen P 1989 The structure and regulation of protein phosphatases. Annu Rev Biochem 58:453-508
12. Wera S, Hemmings BA 1995 Serine/threonine protein phosphatases. Biochem J 311:17-29
13. Sjöholm Ä, Honkanen RE, Berggren P-O 1993 Characterization of serine/threonine protein phosphatases in RINm5F insulinoma cells. Biosci Rep 13:349-358
14. Ratcliff H, Jones PM 1993 Effects of okadaic acid on insulin secretion from rat islets of Langerhans. Biochim Biophys Acta 1175:188-191
15. Mayer P, Jochum C, Schatz H, Pfieffer A 1994 Okadaic acid indicates a major function for protein phosphatases in stimulus-response coupling of RINm5F rat insulinoma cells. Exp Clin Endocrinol 102: 313-319
16. Murphy L, Jones PM 1994 Protein phosphatases PP1, PP2A and PP2B: their presence and function in rat islets of Langerhans. Diabetologia [Suppl 1] 37:A33
17. Ämmälä C, Eliasson L, Bokvist K, Berggren P-O, Honkanen R, Sjöholm Ä, Rorsman P 1994 Activation of protein kinases and inhibition of protein phosphatases play a central role in the regulation of exocytosis in mouse pancreatic β cells. Proc Natl Acad Sci USA 91:4343-4347
18. Cohen P, Holmes CF, Tsukitani Y 1990 Okadaic acid: a new probe for the study of cellular regulation. Trends Biochem Sci 15:98-102
19. Chen J, Martin BL, Brautigan DL 1992 Regulation of protein serine-threonine phosphatase type-2A by tyrosine phosphorylation. Science 257:1261-1264
20. Damuni Z, Xiong H, Li M 1994 Autophosphorylation-activated protein kinase inactivates the protein tyrosine phosphatase 2A. FEBS Lett 352:311-314
21. Lee J, Stock J 1993 Protein phosphatase 2A catalytic subunit is methyl-esterified at its carboxyl terminus by a novel methyltransferase. J Biol Chem 268:19192-19195
22. Xie H, Clarke S 1994 Protein phosphatase 2A is reversibly modified by methyl esterification at its C-terminal leucine residue in bovine brain. J Biol Chem 269:1981-1984
23. Xie H, Clarke S 1994 An enzymatic activity in bovine brain that catalyzes the reversal of the C-terminal methyl esterification of protein phosphatase 2A. Biochem Biophys Res Commun 203:1710-1715
24. Favre B, Zolnierowicz S, Turowski P, Hemmings BA 1994 The catalytic subunit of protein phosphatase 2A is carboxylmethylated in vivo. J Biol Chem 269:16311-16317
25. Li M, Damuni Z 1994 Okadaic acid and microcystin-LR directly inhibit the methylation of protein phosphatase 2A by its specific methylesterase. Biochem Biophys Res Commun 202:1023-1030
26. Floer M, Stock J 1994 Carboxyl methylation of protein phosphatase 2A from Xenopus eggs is stimulated by cAMP and inhibited by okadaic acid. Biochem Biophys Res Commun 198:372-379
27. 2 of a high-affinity GTPase activity in the secretory granules of normal rat and human pancreatic islets. Biochem J 297:399-406
28. Kowluru A, Metz SA 1994 Characterization of nucleoside diphosphokinase activity in human and rodent pancreatic beta cells: evidence for its role in the formation of guanosine triphosphate, a permissive factor for nutrient-induced insulin secretion. Biochemistry 33:12495-12503
29. Kowluru A, Seavey SE, Rhodes CJ, Metz SA 1996 Unique regulation of heterotrimeric GTP-binding proteins in the secretory granules of normal and transformed beta cells. Biochem J 313:97-107
30. Zhang H-J, Walseth TF, Robertson RP 1989 Insulin secretion and cAMP metabolism in HIT cells. Reciprocal and serial passage-dependent relationships. Diabetes 38:44-48
31. Asfari M, Janjic D, Meda P, Li G, Halban PA, Wollheim CB 1992 Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines. Endocrinology 130:167-178
32. Kowluru A, Rabaglia ME, Muse K, Metz SA 1994 Subcellular localization and Kinetic characterization of guanine nucleotide binding proteins in normal rat and human islets and transformed beta cells. Biochim Biophys Acta 1222:348-359
33. Kowluru A, Seavey SE, Rabaglia ME, Metz SA 1995 Non-specific stimulatory effects of mastoparan on pancreatic islet nucleoside diphosphokinase activity: dissociation from insulin secretion. Biochem Pharamacol 49:263-266
34. Metz SA, Rabaglia ME, Stock JB, Kowluru A 1993 Modulation of insulin secretion from normal rat islets by inhibitors of the post-translational modifications of GTP-binding proteins. Biochem J 295: 31-40
35. O'connor CM, Germain BJ 1987 Kinetic and electrophoretic analysis of transmethylation reactions in intact Xenopus laevis oocytes. J Biol Chem 262:10404-10411
36. Uotani K, Naganawa H, Kondo S, Aoyagi T, Umezawa H 1982 Structural studies on ebelactone A and B esterase, produced by actinomycetes. J Antibiot 35:1495-1499
37. Tan EW, Rando RR 1992 Identification of an isoprenylated cysteine methyl ester hydrolase activity in bovine rod outer segment membranes. Biochemistry 31:5572-5578
38. Honkanen RE, Zwiller J, Moore RE, Dailey SL, Khatra BS, Dukelow M, Boynton AL 1991 Cyanobacterial nodularin is a potent inhibitor of type 1 and type 2A protein phosphatases. Mol Pharmacol 40:577-583
39. Guo H, Damuni Z 1993 Autophosphorylation-activated protein kinase phosphorylates and inactivates protein phosphatase 2A. Proc Natl Acad Sci USA 90:2500-2504
40. Takai Y, Kaibuchi K, Kikuchi A, Kawata M 1992 Small GTP binding proteins. Int Rev Cytol 133:187-230
41. Volker C, Miller RA, McClearly WR, Rao A, Poenie M, Backer JM, Stock JB 1991 Effects of farnesylcysteine analogs on protein carboxyl methylation and signal transduction. J Biol Chem 266: 21515-21522
42. Kim Y, Huang J, Cohen P, Matthews J 1993 Protein phosphatases 1, 2A, and 2C are protein histidine phosphatases. J Biol Chem 268: 18513-18518
43. 2+ channels in insulin-secreting cells. Biochem J 298:341-346
44. Sjöholm Ä, Honkanen RE, Berggren P-O 1995 Inhibition of serine/ threonine protein phosphatases by secretagogues in insulin-secreting cells. Endocrinology 136:3391-3397
45. Kowluru A, Li G, Seavey SE, Nesher R, Metz SA 1995 Localization of a methylation-demethylation cycle for GTP-binding proteins in insulin-secreting cells. J Invest Med [Suppl 2] 43:354A