Quantitation of 3′-amino-3′-deoxythymidine (AMT), a toxic catabolite of 3′-azido-3′-deoxythymidine (AZT), by competitive ELISA

Journal of Immunoassay

Volumn 17, Issue 2, 1996, Pages 175-193

  Ferrua, Bernard ^a   Chakboub, Hassan ^b   Roptin, Clotilde ^a   Garraffo, Rodolphe ^a   Faraj, Abdesslem ^d   Grassi, Jacques ^c   Guedj, Roger ^b   Sommadossi, Jean Pierre ^d  

^a UNIVERSITÉ CÔTE D'AZUR   (France)

^b Valrose   (France)

^c CEA SACLAY   (France)

^d UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

3 amino 3 deoxythymidine; 3 azido 3 deoxythymidine; AMT; AZT; ELISA; High Pressure Liquid Chromatography

Indexed keywords

3' AMINO 3' DEOXYTHYMIDINE; ACETIC ACID ETHYL ESTER; BOVINE SERUM ALBUMIN; HORSERADISH PEROXIDASE; IMMUNOGLOBULIN G ANTIBODY; MONOCLONAL ANTIBODY; RABBIT ANTISERUM; ZIDOVUDINE;

ACETYLATION; ADULT; ARTICLE; CLINICAL ARTICLE; CROSS REACTION; DRUG BLOOD LEVEL; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; MALE; ORAL DRUG ADMINISTRATION; SERUM; VIRUS INFECTION;

BINDING, COMPETITIVE; DIDEOXYNUCLEOSIDES; ENZYME-LINKED IMMUNOSORBENT ASSAY; ZIDOVUDINE;

EID: 0029886901     PISSN: 01971522     EISSN: None     Source Type: Journal    
DOI: 10.1080/01971529608005787     Document Type: Article

References (12)

1. Fischl, M.A., Richman, D.D., Grieco, M.H., et al. The efficacy of azidothymidine (AZT) in the treatment of patients with AIDS and AIDS-related complex. N. Engl. J. Med. 1987; 317: 185-91.
2. Schmitt, F.A., Bigley, J.W., McKinnis, R., Logue, P.E., Evans, R.W., and Drucker, J.L. Neuropsychological outcome of zidovudine (AZT) treatment of patients with AIDS and AIDS-related complex. N. Engl. J. Med. 1988; 319: 1573-78.
3. Volberding, P.A., Lagakos, S.W., Koch, M.A., et al. Zidovudine in asymptomatic human immunodeficiency virus infection. N. Engl. J. Med. 1990; 322: 941-49.
4. Richman, D.D., Fischl, M.A., Grieco, M.H. et al. The toxicity of azidothymidine in the treatment of patients with AIDS and AIDS-related complex. N. Engl. J. Med. 1987; 317: 192-97.
5. Cretton, E.M., and Sommadossi, J.P. Reduction of 3′-azido-2′,3′-dideoxynucleosides to their 3′-amino metabolite is mediated by cytochrome P 450 and NADPH-cytochrome P 450 reductase in rat liver microsomes. Drug Metab. Disp. 1993; 21: 946-50.
6. Placidi, L., Cretton, E. M., Placidi, M., and Sommadossi, J.P. Reduction of 3′-azido-3′-deoxythymidine to 3′-amino-3′-deoxythymidine in human liver microsomes and its relationship to cytochrome P 450. Clin. Pharmacol. Ther. 1993; 54: 168-176.
7. Stagg, M.P., Cretton E.M., Kidd, L., Diasio, R.B., and Sommadossi, J.P. Clinical pharmacokinetics of 3′-azido-3′-deoxythymidine (zidovudine) and catabolites with formation of a toxic catabolite, 3′-amino-3′-deoxythymidine. Clin. Pharmacol. Ther. 1992; 51: 668-76.
8. Cretton, E. M., Schinazi, R.F., McClure, H.M., Anderson, D.C., and Sommadossi, J.P. Pharmacokinetics of 3′-azido-3′-deoxythymidine and its catabolites and interactions with probenecid in rhesus monkeys. Antimicrob. Agents Chemother. 1991; 35: 801-7.
9. Cretton, E. M., Xie, M.Y., Bevan, R.J., Goudgaon, N.M., Schinazi, R.F., Sommadossi, J.P. Catabolism of 3′-azido-3′-deoxythymidine in hepatocytes and liver microsomes with evidence of formation of 3′-amino-3′-deoxythymidine, a highly toxic catabolite for human bone marrow cells. Mol. Pharmacol. 1991; 39: 258-66.
10. Zhou, X.J. and Sommadossi, J.P. Quantification of 3′-amino-3′-deoxythymidine, a toxic catabolite of 3′-azido-3′-deoxythymidine (zidovudine) in human plasma by high performance liquid chromatography using precolumn derivatization with fluorescamine and fluorescence detection. J. Chromatogr. 1994; 656: 389-96.
11. Zhou, X.J. and Sommadossi, J.P. Comparative pharmacokinetics of zidovudine and its toxic catabolite 3′-amino-3′deoxythymidine in HIV-infected patients. AIDS Res. Hum. Retroviruses 1995; in press
12. Ferrua, B., Tran, T.T., Quaranta, J.F., et al Measurement of the anti-HIV agent 2′,3′-didehydro2′, 3′-dideoxythymidine (D4T) by competitive ELISA. J. Immunol. Methods 1994; 176: 103-10.