Genetically engineered resistance to dengue-2 virus transmission in mosquitoes

Science

Volumn 272, Issue 5263, 1996, Pages 884-886

  Olson, K E ^a   Higgs, S ^a   Gaines, P J ^a   Powers, A M ^a   Davis, B S ^a   Kamrud, K I ^a   Carlson, J O ^a   Blair, C D ^a   Beaty, B J ^a  

^a Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY RNA; VIRUS VECTOR;

AEDES AEGYPTI; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DENGUE VIRUS; GENETIC ENGINEERING; GENETIC MANIPULATION; MOSQUITO; NONHUMAN; PRIORITY JOURNAL; SALIVARY GLAND; SINDBIS VIRUS; VIRUS RECOMBINANT; VIRUS REPLICATION; VIRUS TRANSMISSION;

AEDES AEGYPTI; ANIMALIA; ARTHROPOD BORNE VIRUS; ARTHROPODA; DENGUE VIRUS; DENGUE VIRUS GROUP; DENGUE VIRUS TYPE 2; SINDBIS VIRUS;

EID: 0029886682     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5263.884     Document Type: Article

References (37)

1. V. Deubel, R. M. Kinney, D. W. Trent, Virology 165, 234 (1988). Y. S. Hahn et al., ibid. 162, 167 (1988).
2. V. Deubel, R. M. Kinney, D. W. Trent, Virology 165, 234 (1988). Y. S. Hahn et al., ibid. 162, 167 (1988).
3. T. J. Chambers, C. S. Hahn, R. Galler, C. M. Rice, Annu. Rev. Microbiol. 44, 649 (1990).
4. S. B Halstead, Science 239, 476 (1988).
5. D. J. Gubler and D. W. Trent, Infect. Agents Dis. 2, 383 (1993).
6. R. H. Whitehead, T. M. Yuill, D. J. Gould, P. Simasathien, Trans. R. Soc. Trop. Med. Hyg. 65, 661 (1971); C. J. Leake, Parasitol. Today 8, 123 (1992).
7. R. H. Whitehead, T. M. Yuill, D. J. Gould, P. Simasathien, Trans. R. Soc. Trop. Med. Hyg. 65, 661 (1971); C. J. Leake, Parasitol. Today 8, 123 (1992).
8. T. P. Monath, Proc. Natl. Acad. Sci. U.S.A. 91, 2395 (1994).
9. J. C. Sanford and S. A. Johnston, J. Theor. Biol. 113, 395 (1985). S. Higgs, A. Powers, K. Olson, Parasitol. Today 9, 444 (1993).
10. J. C. Sanford and S. A. Johnston, J. Theor. Biol. 113, 395 (1985). S. Higgs, A. Powers, K. Olson, Parasitol. Today 9, 444 (1993).
11. A. M. Fallon, Nature 352, 828 (1991).
12. C. S. Hahn, Y. S. Hahn, T. J. Braciale, C. M. Rice, Proc. Natl. Acad. Sci. U.S.A. 89, 2679 (1992); K. E. Olson et al., Insect Biochem. Mol. Biol. 24, 39 (1994).
13. C. S. Hahn, Y. S. Hahn, T. J. Braciale, C. M. Rice, Proc. Natl. Acad. Sci. U.S.A. 89, 2679 (1992); K. E. Olson et al., Insect Biochem. Mol. Biol. 24, 39 (1994).
14. A. Rayms-Keller et al., Insect Mol. Biol. 4, 245 (1995); J. Carlson, K. Olson, S. Higgs, B. Beaty, Annu. Rev. Entomol. 40, 359 (1995).
15. A. Rayms-Keller et al., Insect Mol. Biol. 4, 245 (1995); J. Carlson, K. Olson, S. Higgs, B. Beaty, Annu. Rev. Entomol. 40, 359 (1995).
16. A. M. Powers, K. E. Olson, S. Higgs, J. O. Carlson, B. J. Beaty, Virus Res. 32, 57 (1994).
17. A. M. Powers et al., Proc. Natl. Acad. Sci. U.S.A., in press.
18. P. J. Gaines et al., J. Virol. 70, 2132 (1996).
19. J. M Murray, J. G Aaskov, P. J. Wright, J. Gen. Virol. 74, 175 (1993).
20. B cpm/μg).
21. L. Rosen and D. Gubler, Am. J. Trop. Med. Hyg. 23, 1153 (1974). Aedes aegypti mosquitoes originating from Rexville, Puerto Rico, were obtained from B. Miller, CDC Laboratory. Fort Collins, CO, and were reared at 28°C at 80% relative humidity and with a photoperiod of 16 hours of light and 8 hours of dark.
22. A. M. Powers and K. E. Olson, unpublished results.
23. B. J. Beaty and T. H. G. Aitken, Mosq. News 39, 232 (1979). Immobilized mosquitoes were allowed to probe a 50-μl capillary tube filled with 15 μl of a solution containing 20% bovine fetal serum and 10% sucrose. Saliva was collected for 2 hours, at which time the mosquitoes were removed from the capillary tubes and the saliva transferred to a microfuge tube.
24. The dsSIN viruses in the saliva were neutralized with a mouse SIN polyclonal antibody (MB92) diluted 1:50. Viruses were neutralized for 1 hour at 4°C before each mosquito was intrathoracically injected with 0.5 μl of saliva.
25. Seven days after inoculating mosquitoes with saliva (16), we analyzed the mosquito heads for the presence of DEN-2 E antigen by indirect immunofluorescence assay using a DEN-2-specific mouse polyclonal antibody. The presence of DEN-2 antigen in the mosquitoes indicated the presence of DEN-2 virus in the saliva that escaped interference.
26. J. Crampton, A, Morris, G. Lycett, A. Warren, P. Eggleston, Parasitol. Today 6, 31 (1990); N. J. Besansky and F. H. Collins, ibid. 8, 186 (1992); M. Ashburner, Science 270, 1941 (1995).
27. J. Crampton, A, Morris, G. Lycett, A. Warren, P. Eggleston, Parasitol. Today 6, 31 (1990); N. J. Besansky and F. H. Collins, ibid. 8, 186 (1992); M. Ashburner, Science 270, 1941 (1995).
28. J. Crampton, A, Morris, G. Lycett, A. Warren, P. Eggleston, Parasitol. Today 6, 31 (1990); N. J. Besansky and F. H. Collins, ibid. 8, 186 (1992); M. Ashburner, Science 270, 1941 (1995).
29. P. Chomczynski and N. Sacchi, Anal. Biochem. 162, 156 (1987).
30. J. Sambrook, E. F. Fritsch, T. Maniatis, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, ed. 2, 1989).
31. V. Deubel, R. M. Kinney, D. W. Trent, Virology 155, 365 (1986).
32. D. Marchuk, M. Drumm, A. Saulino, F. S. Collins, Nucleic Acids Res. 19, 1154 (1990).
33. C. M Rice, R. Levis, J. H. Strauss, H. V. Huang, J. Virol. 61, 3809 (1987).
34. G. Karber, Arch. Exp. Pathol. Pharmakol. 162, 480 (1931).
35. A. C. Chanas, E. A. Gould, J. C. S. Clegg, G. R. Varma, J. Gen. Virol. 58, 37 (1982).
36. E. A. Gould et al., ibid. 66, 1369 (1985).
37. We are indebted to C. M. Rice for generously providing the pTE/3′2J construct. This work was supported by the John D, and Catherine T. MacArthur Foundation and NIH grants Al34014 and Al07352.