Cloning, characterization, and regulation of nifF from Rhodobacter capsulatus

Journal of Bacteriology

Volumn 178, Issue 13, 1996, Pages 3949-3952

  Gennaro, Giuseppa ^a   Hübner, Philipp ^b   Sandmeier, Ursula ^b   Yakunin, Alexander F ^a   Hallenbeck, Patrick C ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

^b UNIVERSITY OF BASEL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIA; FLAVODOXIN; HYBRID PROTEIN; IRON; MOLYBDENUM; NITROGENASE;

AMINO ACID SEQUENCE; ARTICLE; AZOTOBACTER VINELANDII; CONTROLLED STUDY; ENZYME ACTIVITY; KLEBSIELLA PNEUMONIAE; NITROGEN FIXATION; NONHUMAN; PRIORITY JOURNAL; RHODOBACTER CAPSULATUS; TRANSCRIPTION REGULATION;

AZOTOBACTER VINELANDII; KLEBSIELLA PNEUMONIAE; RHODOBACTER CAPSULATUS; RHODOPSEUDOMONAS;

EID: 0029885353     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.13.3949-3952.1996     Document Type: Article

References (35)

1. Bennett, L. T., M. R. Jacobson, and D. R. Dean. 1988. Isolation, sequencing, and mutagenesis of the nifF gene encoding flavodoxin from Azotobacter vinelandii. J. Biol. Chem. 263:1364-1369.
2. Colbeau, A., J.-P. Magnin, B. Cauvin, T. Champion, and P. M. Vignais. 1990. Genetic and physical mapping of an hydrogenase gene cluster from Rhodobacter capsulatus. Mol. Gen. Genet. 220:393-399.
3. Deistung, J., F. C. Cannon, M. C. Cannon, S. Hill, and R. N. F. Thorneley. 1985. Electron transfer to nitrogenase in Klebsiella pneumoniae; nifF gene cloned and the gene product, a flavodoxin, purified. Biochem. J. 231:743-753.
4. Drummond, M. H. 1985. The sequence of the nifF gene of Klebsiella pneumoniae and homology of the predicted amino acid sequence of its product to other flavodoxins. Biochem. J. 232:891-896.
5. Fonstein, M., S. Zheng, and R. Haselkorn. 1992. Physical map of the genome of Rhodobacter capsulatus SB 1003. J. Bacteriol. 174:4070-4077.
6. Gennaro, G., A. F. Yakunin, and P. C. Hallenbeck. 1994. Cloning and characterization of nifF from Rhodobacter capsulatus, abstr. 51B, p. 51. In Eighth International Symposium on Phototrophic Prokaryotes. Urbino, Italy.
7. Grabau, C., E. Schatt, Y. Jouanneau, and P. M. Vignais. 1991. A new [2Fe-2S] ferredoxin from Rhodobacter capsulatus. Coexpression with a 2[4Fe-4S] ferredoxin in Escherichia coli. J. Biol. Chem. 266:3294-3299.
8. Hallenbeck, P. C., Y. Jouanneau, and P. M. Vignais. 1982. Purification and molecular properties of a soluble ferredoxin from Rhodopseudomonas capsulata. Biochim. Biophys. Acta 681:158-176.
9. Hill, S., and E. P. Kavanagh. 1980. Roles of nifF and nifl gene products in electron transport to nitrogenase in Klebsiella pneumoniae. J. Bacteriol. 141: 470-475.
10. 2 production by growing cultures. J. Bacteriol. 129:724-731.
11. Hübner, P., B. Masepohl, W. Klipp, and T. A. Bickle. 1993. nif gene expression studies in Rhodobacter capsulatus: ntrC-independent repression by high ammonium concentrations. Mol. Microbiol. 10:123-132.
12. Hübner, P., J. C. Willison, P. M. Vignais, and T. A. Bickle. 1991. Expression of regulatory nif genes in Rhodobacter capsulatus. J. Bacteriol. 173:2993-2999.
13. Jacobson, M. R., K. E. Brigle, L. T. Bennett, R. A. Setterquist, M. S. Wilson, V. L. Cash, J. Beynon, W. E. Newton, and D. R. Dean. 1989. Physical and genetic map of the major nif gene cluster from Azotobacter vinelandii. J. Bacteriol. 171:1017-1027.
14. Jouanneau, Y., C. Meyer, I. Naud, and W. Klipp. 1995. Characterization of an fdxN mutant of Rhodobacter capsulatus indicates that ferredoxin I serves as electron dunor to nitrogenase. Biochim. Biophys. Acta 1232:33-42.
15. 2 fixation, p. 472. In R. Palacios, J. Mora, and W. E. Newton (ed.), New horizons in nitrogen fixation: proceedings of the 9th International Congress on Nitrogen Fixation. Kluwer Academic Publishers, Dordrecht, The Netherlands.
16. Klipp, W., B. Masepohl, and A. Pühler. 1988. Identification and mapping of nitrogen fixation genes of Rhodobacter capsulatus: duplication of a nifA-nifB region. J. Bacteriol. 170:693-699.
17. Kranz, R. G., and D. Foster-Hartnett. 1990. Transcriptional regulatory cas-cade of nitrogen-fixation genes in anoxygenic photosynthetic bacteria: oxygen- and nitrogen-responsive factors. Mol. Microbiol. 4:1793-1800.
18. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
19. Nieva-Gomez, D., G. P. Roberts, S. Klevickis, and W. J. Brill. 1980. Electron transport to nitrogenase in Klebsiella pneumoniae. Proc. Natl. Acad. Sci. USA 77:2555-2558.
20. Roberts, G. P., T. MacNeil, D. MacNeil, and W. J. Brill. 1978. Regulation and characterization of protein products coded by the nif (nitrogen fixation) genes of Klebsiella pneumoniae. J. Bacteriol. 136:267-279.
21. Saeki, K., Y. Suetsugu, K.-I. Tokuda, Y. Miyatake, D. A. Young, B. L. Marrs, and H. Matsubara. 1991. Genetic analysis of functional difference among distinct ferredoxins in Rhodobacter capsulatus. J. Biol. Chem. 266:12886-12895.
22. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
23. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
24. Schatt, E., Y. Jouanneau, and P. M. Vignais. 1989. Molecular cloning and sequence analysis of the structural gene of ferredoxin I from the photosynthetic bacterium Rhodobacter capsulatus. J. Bacteriol. 171:6218-6226.
25. 2-fixing bacteria. Anal. Biochem. 193:292-298.
26. Schneider, K., A. Müller, U. Schramm, and W. Klipp. 1991. Demonstration of a molybdenum- and vanadium-independent nitrogenase in a nifHDK-deletion mutant of Rhodobacter capsulatus. Eur. J. Biochem. 195:653-661.
27. Schüddekopf, K., S. Hennecke, U. Liese, M. Kutsche, and W. Klipp. 1993. Characterization of anf genes specific for the alternative nitrogenase and identification of nif genes required for both nitrogenases in Rhodobacter capsulatus. Mol. Microbiol. 8:673-684.
28. Shah, V. K., G. Stacey, and W. J. Brill. 1983. Electron transport to nitrogenase: purification and characterization of pyruvate:flavodoxin oxidoreductase, the nifJ gene product. J. Biol. Chem. 258:12064-12068.
29. 2. J. Bacteriol. 121:759-765.
30. Wall, J. D., and K. Braddock. 1984. Mapping of Rhodopseudomonas capsulata nif genes. J. Bacteriol. 158:404-410.
31. Weaver, P. F., J. D. Wall, and H. Gest. 1975. Characterization of Rhodopseudomonas capsulata. Arch. Microbiol. 105:207-216.
32. Willison, J. C., G. Ahombo, J. Chabert, J. P. Magnin, and P. M. Vignais. 1985. Genetic mapping of the Rhodopseudomonas capsulata chromosome shows non-clustering of genes involved in nitrogen fixation. J. Gen. Microbiol. 131:3001-3015.
33. Willison, J. C., and P. M. Vignais. 1982. The use of metronidazole to isolate Nif mutants of Rhodopseudomonas capsulata, and the identification of a mutant with altered regulatory properties of nitrogenase. J. Gen. Microbiol. 128:3001-3010.
34. Yakunin, A. F., G. Gennaro, and P. C. Hallenbeck. 1993. Purification and properties of a nif-specific flavodoxin from the photosynthetic bacterium Rhodobacter capsulatus. J. Bacteriol. 175:6775-6780.
35. Yakunin, A. F., and I. N. Gogotov. 1983. Properties and regulation of the synthesis of two ferredoxins from Rhodopseudomonas capsulata. Biochim. Biophys. Acta 725:298-308.