Peptic-tryptic digests of gliadin: Contaminating trypsin but not pepsin interferes with gastrointestinal protein binding characteristics

Clinica Chimica Acta

Volumn 247, Issue 1-2, 1996, Pages 59-70

  Bolte, Gabriele ^a   Osman, Awad ^b   Mothes, Thomas ^b   Stern, Martin ^a  

^a UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^b UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

brush border membranes; coeliac disease; Frazer's fraction III; gliadin peptic tryptic digests

Indexed keywords

GLIADIN; PEPSIN A; TRYPSIN;

ANIMAL TISSUE; ARTICLE; CHEMOLUMINESCENCE; CONTROLLED STUDY; DRUG CONTAMINATION; DRUG PURITY; FEMALE; IMMUNOBLOTTING; INTESTINE BRUSH BORDER MEMBRANE; MEMBRANE BINDING; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALIA;

EID: 0029884965     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/0009-8981(95)06220-3     Document Type: Article

References (29)

1. Frazer AC, Fletcher RF, Ross CA, Shaw B, Sammons HG, Schneider R. Gluten-induced enteropathy. The effect of partially digested gluten. Lancet 1959;2:252-255.
2. Auricchio S, De Ritis G, De Vincenzi M et al. Amines protect in vitro the celiac small intestine from damaging activity of gliadin peptides. Gastroenterology 1990;99:1668-1674.
3. Stallmach A, Belitz HD, Gellermann B, Schäfer H, Wieser H, Stern M. Effects of gliadin peptides B1-B4 in celiac disease. I. Organ culture studies. J Pediatr Gastroenterol Nutr 1987;6:335-340.
4. + T cells and macrophages but no crypt-cell hyperplasia. Scand J Immunol 1993;38:581-590.
5. Leigh RJ, Marsh MN, Crowe PJ, Garner V, Gordon D. Studies of intestinal lymphoid tissue. IX. Dose-dependent gluten-induced lymphoid infiltration of coeliac jejunal epithelium. Scand J Gastroenterol 1985;20:715-719.
6. Marsh MN, Loft DE, Garner VG, Gordon D. Time/dose responses of coeliac mucosae to graded oral challenges with Frazer's fraction III (FF3) of gliadin. Eur J Gastroenterol Hepatol 1992;4:667-674.
7. Unsworth DJ, Würzner R, Brown DL, Lachmann PJ. Extracts of wheat gluten activate complement via the alternative pathway. Clin Exp Immunol 1993;94:539-543.
8. Colyer J, Kumar PJ, Waldron NM, Clark ML, Farthing MJG. Gliadin binding to rat and human enterocytes. Clin Sci 1987;72:593-598.
9. Rühlmann J, Sinha P, Hansen G, Tauber R, Köttgen E. Studies on the aetiology of coeliac disease: no evidence for lectin-like components in wheat gluten. Biochim Biophys Acta 1993;1181:249-256.
10. Wieser H, Belitz HD, Ashkenazi A, Idar D. Isolation of coeliac active peptide fractions from gliadin. Z Lebensm Unters Forsch 1983;176:85-94.
11. Wieser H, Belitz HD. Coeliac active peptides from gliadin: large-scale preparation and characterization. Z Lebensm Unters Forsch 1992;194:229-234.
12. Osman AA, Braunersreuther I, Mothes T. Investigation of gliadin binding to different selected proteins using a biotin-streptavidin system. Z Lebensm Unters Forsch 1994;198:249-252.
13. Mothes T, Bendix U, Pfannschmidt C, Lehmann I. Effect of gliadin and other food peptides on expression of MHC-class II molecules by HT-29 cells. Gut 1995;36:548-552.
14. Kessler M, Acuto O, Storelli C, Müller M, Semenza G. A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of D-glucose and choline transport systems. Biochim Biophys Acta 1978;506:136-154.
15. Dahlqvist A. Assay of intestinal disaccharidases. Scand J Clin Lab Invest 1984;44:169-172.
16. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-275.
17. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-685.
18. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350-4354.
19. Batteiger B, Newhall WJ, Jones RB. The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. J Immunol Methods 1982;55:297-307.
20. Durrant I. Light-based detection of biomolecules. Nature 1990;346:297-298.
21. Hawkes R, Niday E, Gordon J. A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem 1982;119:142-147.
22. Bolte G, Knauss M, Metzdorf I, Stern M. Development of a new test system for studying protein binding to small intestinal brush border membranes. Gut 1994;35(Suppl. 4):A196.
23. Stern M, Gellermann B, Belitz HD, Wieser H. Food proteins and maturation of small intestinal microvillus membranes (MVM): II. Binding of gliadin hydrolysate fractions and of gliadin peptide B3142. J Pediatr Gastroenterol Nutr 1988;7:122-127.
24. Pittschieler K, Ladinser B, Petell J K. Reactivity of gliadin and lectins with celiac intestinal mucosa. Pediatr Res 1994;36:635-641.
25. Köttgen E, Beiswenger M, James LF, Bauer C. In vivo induction of gliadin-mediated enterocyte damage in rats by the mannosidase inhibitor, swainsonine: a possible animal model for coeliac disease. Gastroenterology 1988;95:100-106.
26. Friis S, Dabelsteen E, Sjöström H, Norén O, Jarnum S. Gliadin uptake in human enterocytes: differences between coeliac patients in remission and control individuals. Gut 1992;33:1487-1492.
27. Lundin KEA, Scott H, Hansen T et al. Gliadin-specific, HLA-DQ(a1*0501, b1*0201) restricted T cells isolated from the small intestinal mucosa of celiac disease patients. J Exp Med 1993;178:187-196.
28. Cornell HJ, Mothes T. The activity of wheat gliadin peptides in in vitro assays for coeliac disease. Biochim Biophys Acta 1993;1181:169-173.
29. 1-antitrypsin deficiency. Lancet 1982;I:1441-1443.