Coordination of glycolysis and TCA cycle reaction networks: Citrate-glucose cometabolism eliminates acids and reveals potential metabolic engineering strategies

Annals of the New York Academy of Sciences

Volumn 782, Issue , 1996, Pages 1-16

  Goel, A ^a   Domach, M M ^b   Hanley, William ^a   Lee, J W ^b   Ataai, M M ^a  

^a University of Pittsburgh   (United States)

^b CARNEGIE MELLON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; CITRIC ACID; GLUCOSE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GLUCOSE 6 PHOSPHATE ISOMERASE; GLUTAMINE; ISOCITRATE DEHYDROGENASE; MALATE DEHYDROGENASE (DECARBOXYLATING); PYRUVATE CARBOXYLASE; PYRUVATE DEHYDROGENASE; RECOMBINANT PROTEIN;

BACTERIUM CULTURE; CHEMOSTAT; CITRIC ACID CYCLE; CONFERENCE PAPER; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SYNTHESIS; GLYCOLYSIS; NONHUMAN; OPTICAL DENSITY;

BACTERIA (MICROORGANISMS);

EID: 0029882979     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.1996.tb40542.x     Document Type: Article

References (38)

1. HAN, K., H. C. LIM & J. HONG. 1992. Acetic acid formation in Escherichia coli fermentation. Biotechnol. Bioeng. 39: 663-671.
2. MAJEWSKI, R. A. & M. M. DOMACH. 1990. Simple constrained-optimization view of acetate overflow in E. coli. Biotechnol. Bioeng. 35: 732-738.
3. INGLEDEW, W. J. & R. K. POOLE. 1984. The respiratory chains of Escherichia coli. Microbiol. Rev. 48: 222-271.
4. JENSEN, K. F. & S. PEDERSON. 1990. Metabolic growth rate control in Escherichia coli may be a consequence of subsaturation of the macromolecular appararus svith substrates and catalytic components. Microbial. Rev. 54: 89-100.
5. MARR, A. G. 1991. Growth rate of Escherchia coli. Microbiol. Rev. 55: 316-333.
6. GOEL, A., J-W. LEE, M. M. DOMACH & M. M. ATAAI. 1995. Suppressed acid formation by co-feeding glucose and citrate in Bacillus cultures: Emergence of pyruvate kinase as a potential metabolic engineering site. Biotechnol. Prog. 11: 380-386.
7. VIERHELLER, C., A. GOEL, M. M. DOMACH & M. M. ATAAI. 1995. Sustained and constitutive high levels of protein production in continuous cultures of B. subtilis. Biotechnol. Bioeng. 47: 520-524.
8. GOEL, A., M. M. DOMACH & M. M. ATAAI. 1995. Several-fold reduction in oxygen requirements, and substantial increase in cellular and product yields by tuning of glycolytic flux. Manuscript in preparation.
9. WILLECKE, K. & A. B. PARDEE. 1971. Inducible transport of citrate in gram-positive bacteria, B. subtilis. J. Biol. Chem. 246: 1032-1040.
10. LEHNINGER, A. L. 1975. Biochemistry. 2nd edit. Worth Publishers Inc. New York. p. 431.
11. BOITEUX, A., M. MARKUS, T. PLESSER, B. HESS & M. MALCOVATI. 1983. Interaction of pyruvate kinase from Escherichia coli with fructose 1,6-biphosphate and calcium ions. Biochem. J. 211: 631-640.
12. GOEL, A., M. M. DOMACH & M. M. ATAAI. 1995. Suppressed acid formation in bacterial chemostats: Reduced PYK and PFK fluxes and enhanced phosphorylated metabolite pools. Submitted.
13. STEPHANOPOULOS, G. & J. L. VALLINO. 1991. Network rigidity and metabolic engineering in metabolite overproduction. Science 252: 1675-1681.
14. VARMA, A., B. W. BOESCH & B. O. PALSSON. 1993. Biochemical production capabilities of Escherichia coli. Biotechnol. Bioeng. 42: 59-73.
15. WALSH, K. & D. E. KOSHLAND, JR. 1984. Determination of flux through the branch point of two metabolic cycles. The tricarboxylic acid cycle and the glyoxylate shunt. J. Biol. Chem. 259: 9646-9654.
16. WALSH, K. & D. E. KOSHLAND, JR. 1985. Branch point control by the phosphorylation state of isocitrate dehydrogenase. A quantitative examination of fluxes during a regulatory transition. J. Biol. Chem. 260: 8430-8437.
17. LEE, J., A. GOEL, M. M. ATAAI & M. M. DOMACH. 1995. Supply side optimization analysis of citrate-glucose cometabolism by B. subtilis: Feasible network alternatives, regulatory shifts and yield optimality. Biotechnol. Bioeng. Submitted.
18. FELL, D. A. & J. R. SMALL. 1986. Fat synthesis in adipose tissue: An examination of stoichiometric constraints. Biochem. J. 238: 781-786.
19. LEGRICE, S. F. J. 1990. A regulated promoter for high level expression of heterologous genes in Bacillus subtilis. Methods Enzymol. 185: 201.
20. LEGRICE, S. F. J., R. GENTZ, U. PESCHKE, D. STUBER, V. BEUCK & H. BUJARD. 1986. Intergenic utilization of Escherichia coli: Transcriptional signals. In A. T. Ganesan & J. A. Hoch, Eds.: 433-445. Bacilllus Molecular Genetics and Biotechnology Applications. Academic Press. New York.
21. BEITLE, R. R. & M. M. ATAAI. 1991. A unique cultivation monitoring and control system. Biotechnol. Tech. 5: 77-82.
22. GOEL, A., J. FERRANCE, J. W. JEONG & M. M. ATAAI. 1993. Analysis of metabolic fluxes in batch and continuous cultures of Bacillus subtilis. Biotechnol. Bioeng. 42: 686-696.
23. FISHER, S. H. & B. MAGASANIK. 1984. Synthesis of oxaloacetate in Bacillus subtilis mutants lacking the α-ketoglutarate dehydrogenase enzyme complex. J. Bacteriol. 158: 55-62.
24. YOUNG, M. R., B. TOLBERT & M. F. UTTER. 1969. Pyruvate carboxylase from Saccharomyces cerevisiae. Methods Enzymol. 13: 250-258.
25. YOSHIDA, A. 1969. L-Malate dehydrogenase from B. subtilis. Methods Enzymol. 13: 141-147.
26. NOLTMANN, E. A. 1966. Phosphoglucose isomerase. Methods Enzymol. 19: 557-565.
27. REED, L. J. & J. A. WILLMS. 1966. Purification and resolution of the pyruvate dehydrogenase complex (Escherichia coli). Methods Enzymol. 9: 247-265.
28. HSU, R. Y. & H. A. LARDY. 1969. Malic enzyme. Methods Enzymol. 13: 230-235.
29. RODRIGUEZ, R. L. & R. C. TAIT. 1983. Recombinant DNA Techniques: An Introduction. The Benjamin/Cummings Publishing Company, Inc. Menlo Park, CA. p. 187-192.
30. INGRAHAM, J. L., O. MAALØE & F. C. NEIDHARDT. 1983. Growth of the Bacterial Cell. Sinauer Associates Inc. Sunderland, MA.
31. WILLECKE, K., E. GRIES & P. OEHR. 1973. Coupled transport of citrate and magnesium in Bacillus subtilis. J. Biol. Chem. 248: 807-814.
32. DOELLE, H. W., K. N. EWINGS & N. W. HOLLYWOOD. 1982. Regulation of glucose metabolism in bacterial systems. Adv. Biochem. Eng. 23: 1-35.
33. GANESAN, A. T. & J. A. HOCH, Eds. 1988. Genetics and Biotechnology of Bacilli. Academic Press. New York.
34. HOCH, J. A. & P. SETLOW, Eds. 1985. Molecular Biology of Microbial Differentiation. American Society for Microbiology. Washington, DC.
35. ATAAI, M. M., W. STROHM & J. W. JEONG. 1989. Protease problem in relation to B. subtilis as a host of recombinant vectors. In Frontiers in Bioprocessing. M. Bier, P. Todd & S. K. Sikdar, Eds.: 167-172. CRC Press. Boca Raton, FL.
36. PRIEST, F. G. 1977. Extracellular enzyme synthesis in the genus Bacillus. Bacteriol. Rev. 41: 711-720.
37. HEINEKEN, F. G. & R. J. O'CONNOR. 1972. Continuous culture studies on the biosynthesis of alkaline protease, neutral protease and α-amylase by Bacillus subtilis NRRL-B3411. J. Gen. Microbiol. 73: 35-44.
38. ZABRISKIE, D. W. & E. J. ARCURI. 1986. Factors influencing productivity of fermentations employing recombinant microorganisms. Enzyme Microb. Technol. 16: 933-941.