The binding site on cochlear stereocilia for antisera raised against renal Na+ channels is blocked by amiloride and dihydrostreptomycin

Hearing Research

Volumn 93, Issue 1-2, 1996, Pages 136-146

  Furness, David N ^a   Hackney, Carole M ^a   Benos, Dale J ^b  

^a KEELE UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

aminoglycoside; auditory; cochlea; epithelial Na+ channel; mechanotransduction

Indexed keywords

AMILORIDE; DIHYDROSTREPTOMYCIN; SODIUM CHANNEL;

ANIMAL TISSUE; ARTICLE; COCHLEA; FEMALE; GUINEA PIG; IMMUNOFLUORESCENCE MICROSCOPY; MALE; NONHUMAN; PRIORITY JOURNAL; STEREOCILIUM;

EID: 0029882623     PISSN: 03785955     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-5955(95)00224-3     Document Type: Article

References (26)

1. Ashmore, J.F. and Ohmori, H. (1990) Control of intracellular calcium by ATP in isolated outer hair cells of the guinea-pig cochlea. J. Physiol. Lond. 428, 109-131.
2. + channels in rat kidney medulla. Am. J. Physiol. 256, F366-F369.
3. Denk, W., Keolian, R.M. and Watt, W.W. (1992) Mechanical response of frog saccular hair bundles to aminoglycoside block of mechanoelectrical transduction. J. Neurophysiol. 68, 927-932.
4. Furness, D.N. and Hackney, C.M. (1985) Cross links between stereocilia in the guinea pig cochlea. Hear. Res. 18, 177-188.
5. Garty, H. and Edelman, S. (1983) Amiloride-sensitive trypsinization of apical sodium channels. Analysis of hormonal regulation of sodium transport in toad bladder. J. Gen. Physiol. 81, 785-803.
6. Goodyear, R. and Richardson, G.P. (1992) Distribution of the 275 kDa hair cell antigen and cell surface specializations on auditory and vestibular hair bundles in the chicken inner ear. J. Comp. Neurol. 325, 243-256.
7. de Groot, J.C.M.J and Veldman, J.E. (1988) Early effects of gentamicin on cochlear glycocalyx cytochemistry. Hear. Res. 35, 39-46.
8. Hackney, C.M. and Furness, D.N. (1995) Mechanotransduction in vertebrate hair cells: structure and function of the stereociliary bundle. Am. J. Physiol. 268, C1-C13.
9. Hackney, C.M., Furness, D.N. and Benos, D.J. (1991) Localization of putative mechanoelectrical transducer channels in cochlear hair cells by immunoelectron microscopy. Scan. Microsc. 5, 741-746.
10. Hackney, C.M., Furness, D.N., Benos, D.J., Woodley, J.F. and Barratt, J. (1992) Putative immunolocalization of the mechanoelectrical transduction channels in mammalian cochlear hair cells. Proc. R. Soc. bond. B. 248, 215-221.
11. Hackney, C.M., Furness, D.N. and Mahendrasingam, S. (1993) The mechanotransduction channels in cochlear hair cells may be revealed by antibodies which recognise other amiloride-sensitive channels. In: H. Duifhuis, J.W. Horst, P. van Dijk and S.M. van Netten (Eds.), Biophysics of Hair Cell Sensory Systems, World Scientific, Singapore, pp. 107-115.
12. Housley, G.D., Greenwood, D, and Ashmore, J. (1992) Localization of cholinergic and purinergic receptors on outer hair cells isolated from the guinea-pig cochlea. Proc. R. Soc. Lond. B. 249, 265-273.
13. Housley, G.D., Greenwood, D., Mockett, B.G., Munoz, D.J.B. and Thorne, P.R. (1993). Differential actions of ATP-activated conductances in outer and inner hair cells isolated from the guinea-pig organ of Corti: a humoral purinergic influence on cochlear function. In: H. Duifhuis, J.W. Horst, P. van Dijk, and S.M. van Netten (Eds.), Biophysics of Hair Cell Sensory Systems, World Scientific, Singapore, pp. 116-123.
14. Huang, P.L. and Corey, D.P. (1990) Calcium influx into hair-cell stereocilia: further evidence for transduction channels at the tips. Biophys. J. 57, 530a.
15. Hudspeth, A.J. (1982) Extracellular current flow and the site of transduction by vertebrate hair cells. J. Neurosci. 2, 1-10.
16. Hudspeth, A.J. (1992) Hair bundle mechanics and a model for mechanotransduction by hair cells. In: D.P. Corey and S.D. Roper (Eds.), Sensory Transduction, Rockefeller University Press, New York, pp. 358-370.
17. Jaramillo, F. and Hudspeth, A.J. (1991) Localization of the hair cell's transduction channels at the hair bundle's top by iontophoretic application of a channel blocker. Neuron 7, 409-420.
18. Jorgensen, F. and Ohmori, H. (1988) Amiloride blocks the mechano-electrical transduction channel of hair cells of the chick. J. Physiol. Lond. 403, 577-588.
19. + channel antibody raised by an antiidiotypic route. J. Biol. Chem. 266, 3907-3915.
20. Kössl, M., Richardson, G.P. and Russell, I.J. (1990) Stereocilia bundle stiffness: effects of neomycin sulphate, A23187 and concanavalin A. Hear. Res. 44, 217-230.
21. Kroese, A.B.A., Das, A. and Hudspeth, A.J. (1989) Blockage of the transduction channels of hair cells in the bullfrog's sacculus by aminoglycoside antibiotics. Hear. Res. 37, 203-218.
22. Pickles, J.O., Comis, S.D. and Osborne, M.P. (1984) Cross-links between stereocilia in the guinea-pig organ of Corti, and their possible relation to sensory transduction. Hear. Res. 15, 103-112.
23. Pickles, J. O. and Corey, D.P. (1992) Mechanoelectrical transduction by hair cells. TINS 15, 254-259.
24. Richardson, G.P., Russell, I.J., Wasserkort, R. and Hans, M. (1988) Aminoglycoside antibiotics and lectins cause irreversible increases in the stiffness of cochlear hair cell stereocilia. In: J.P. Wilson and D.T. Kemp (Eds.). Cochlear Mechanisms, Plenum, London, pp. 57-65.
25. Rüsch, A., Kros, C.J., and Richardson, G.P. (1994) Block by amiloride and its derivatives of mechano-electrical transduction in outer hair cells of mouse cochlear cultures. J. Physiol. Lond. 474, 75-86.
26. Tousson, A., Alley, C.D., Sorscher E.J., Brinkley, B.R. and Benos, D.J. (1989) Immunochemical localization of amiloride-sensitive sodium channels in sodium-transporting epithelia. J. Cell Sci. 93, 349-362.