Connexin43 gap junctions exhibit asymmetrical gating properties

Pflugers Archiv European Journal of Physiology

Volumn 431, Issue 5, 1996, Pages 775-785

  Banach, Kathrin ^a,b   Weingart, Robert ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b SUNY UPSTATE MEDICAL UNIVERSITY   (United States)

Author keywords

Biophysical properties; Connexin43; Gap junctions; Pancreas; Rat; RIN cells; Voltage gating

Indexed keywords

GAP JUNCTION PROTEIN; ION CHANNEL;

ANIMAL CELL; ARTICLE; CELL COMMUNICATION; CELL JUNCTION; CELL MEMBRANE POTENTIAL; CONDUCTANCE; GENETIC TRANSFECTION; NONHUMAN; PANCREAS ISLET CELL TUMOR; PRIORITY JOURNAL; RAT; VOLTAGE CLAMP;

ADENOMA, ISLET CELL; ANIMALS; BIOPHYSICS; CONNEXIN 43; ION CHANNEL GATING; ION CHANNELS; KINETICS; MEMBRANE POTENTIALS; PATCH-CLAMP TECHNIQUES; PULSE; RATS; TUMOR CELLS, CULTURED;

EID: 0029881970     PISSN: 00316768     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004240050065     Document Type: Article

References (37)

1. Anumonowo JMB, Wang HZ, Trabka-Janik E, Dunhum B, Veenstra RD, Delmar M, Jalife J (1992) Gap junctionul channels in adult mammalian sinus nodal cells. Circ Res 71:229-239
2. 1-gating depends on the pulse protocol. Experientia 50:A39
3. Banach K, Weingart R, Meda P (1993) Characterisation of Cx43-gap junctions in transfected cells. Pflügers Arch 422:R90
4. j-dependent inactivation of cardiac gap junction channels. Biophys J 66:A259
5. Bennett MV, Barrio LC, Bargiello TA, Spray DC, Hertzberg E, Saez JC (1991 ) Gap junctions: new tools, new answers, new questions. Neuron 6:305-320
6. Beyer EC, Paul D, Goodenough DA (1987) Connexin43: a protein from rat heart homologous to a gap junction protein from liver. J Cell Biol 105:2621-2629
7. Bukauskas FF, Weingart R (1993) Temperature dependence of gap junction properties in neonatal rat heart cells. Pflügers Arch 423:133-139
8. Bukauskas FF, Weingart R (1994) Voltage-dependent gating of single gap junction channels in an insect cell line. Biophys J 67:613-625
9. Bukauskas F, Kempf C, Weingart R (1992) Electrical coupling between cells of the insect Aedes albopictus. J Physiol (Lond) 448:321-337
10. Bukauskas FF, Elfgang C, Willecke K, Weingart R (1995) Biophysical properties of gap junction channels formed by mouse connexin40 in induced pairs of transfected human HeLa cells. Biophys J 68:2289-2298
11. Chen YH, DeHaan RL (1992) Multiple channel conductance states and voltage regulation of embryonic chick cardiac gap junctions. J Membr Biol 127:95-111
12. Eckert R, Dunina-Barkovskaya A, Hülser DF (1993) Biophysical characterization of gap-junction channels in HeLa cells. Pflügers Arch 424:335-342
13. Fishman GI, Spray DC, Leinwand LA (1990) Molecular characterization and functional expression of the human cardiac gap junction channel. J Cell Biol 111:589-598
14. Gazdar AF, Chick WL, Oie HK, Sims HL, King DL, Weir GC, Lauris V (1980) Continuous, clonal, insulin-and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor. Proc Natl Aead Sci USA 77:3519-3523
15. Harris AL, Spray DC, Bennett MV (1981) Kinetic properties of a voltage-dependent junctional conductance. J Gen Physiol 77:95-117
16. Hille B (1992) Ionic channels of excitable membranes. Sinauer, Sunderland, Mass., USA, pp 23-83
17. Lal R, Arnsdorf MF (1992) Voltage-dependent gating and single-channel conductance of adult mammalian atrial gap junctions. Circ Res 71:737-743
18. Meda P, Chanson M, Pepper M, Giordano E, Bosco D, Traub O, Willecke K, el Aoumari A, Gros D, Beyer EC (1991) In vivo modulation of connexin 43 gene expression and junctional coupling of pancreatic B-cells. Exp Cell Res 192:469-480
19. Moreno AP, Eghbali B, Spray DC (1991) Connexin32 gap junction channels in stably transfected cells. Equilibrium and kinetic properties. Biophys J 60:1267-1277
20. Niggli E, Rüdisüli A, Maurer P, Weingart R (1989) Effects of general anesthetics on current flow across membranes in guinea pig myocytes. Am J Physiol 256:C273-C281
21. Perez-Armendariz EM, Romano MC, Luna J, Miranda C, Bennett MV, Moreno AP (1994) Characterization of gap junctions between pairs of Leydig cells from mouse testis. Am J Physiol 267:C570-C580
22. Rook MB, Jongsma HJ, van Ginneken AC (1988) Properties of single gap junctional channels between isolated neonatal rat heart cells. Am J Physiol 255:H770-H782
23. Rorsman P, Trube G (1986) Calcium and delayed potassium currents in mouse pancreatic b-cells under voltage-clamp conditions. J Physiol (Lond) 374:531-550
24. Rubin JB, Verselis VK, Bennett MVL, Bargiello TA (1992) A novel domain substitution procedure and its use to analyze voltage dependence of homotypic gap junctions formed by connexin 26 and 32. Proc Natl Acad Sci USA 89:3820-3824
25. Sigworth FJ (1993) Voltage gating of ion channels. Q Rev Biophysics 27:1-40
26. Suchyna TM, Xian Xu L, Gao F, Fourtner CR, Nicholson BJ (1993) Identification of a proline residue as a transduction element involved in voltage gating of gap junctions. Nature (Lond) 365:847-849
27. Swenson KI, Jordan JR, Beyer EC, Paul DL (1989) Formation of gap junctions by expression of connexins in Xenopus oocyte pairs. Cell 57:145-155
28. Veenstra RD (1990) Voltage-dependent gating of gap junctional conductance in embryonic chick heart. Ann N Y Acad Sci 588:93-105
29. Verselis VK, Bennett MV, Bargiello TA (1991) A voltage-dependent gap junction in Drosophila melanogaster. Biophys J 59:114-126
30. Verselis VK, Ginter CS, Bargiello TA (1994) Opposite voltage gating polarities of two closely related connexins. Nature 368:348-351
31. Vozzi C, Ullrich S, Bosco D, Dupont E, Meda P (1994) Glucose-unresponsive insulin-producing cells show defects in gap junction and improved secretion after Cx43 transfection. Acta Anat 149:162
32. Wang HZ, Li J, Lemanski LF, Veenstra RD (1992) Gating of mammalian cardiac gap junction channels by transjunctional voltage. Biophys J 63:139-151
33. Waltzman MN, Spray DC (1995) Exogenous expression of connexins for physiological characterization of channel properties: comparison of methods and results. In: Kanno Y, Kataoka K, Shiba Y, Shibata Y, Shimazu T (eds) Progress in cell research, vol 4. Intercellular communication through gap junctions. Elsevier, Amsterdam, pp 9-17
34. Weingart R (1986) Electrical properties of the nexal membrane studied in rat ventricular cell pairs. J Physiol (Lond) 370: 267-284
35. Weingart R (1993) Gating properties of cardiac gap junctions. Abstracts of the XXXII Congress of the International Union of Physiological Sciences, 337.210; Glasgow, Scotland, August 16, 1993
36. White TW, Bruzzone R, Wolfram S, Paul DL, Goodenough DA (1994) Selective interactions among the multiple connexin proteins expressed in the vertebrate lens: the second extracellular domain is a determinant of compatibility between connexins. J Cell Biol 125:879-892
37. Wilders R, Jongsma HJ (1992) Limitations of the dual voltage clamp method in assaying conductance and kinetics of gap junction channels. Biophys J 63:942-953