Immunofluorescent artifacts due to the pH of antifading mounting media

BioTechniques

Volumn 20, Issue 3, 1996, Pages 398-400

  Swartz, Daniel J ^a   Santi, Peter A ^a,b  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^b Lions Research Laboratories   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN DETECTION; ARTICLE; ARTIFACT; COCHLEA; DIAGNOSTIC ERROR; IMMUNOFLUORESCENCE TEST; IMMUNOHISTOCHEMISTRY; NONHUMAN; PHOTOSENSITIVITY; RABBIT;

ANIMALS; ANTIBODIES, ANTINUCLEAR; ANTIBODY SPECIFICITY; ARTIFACTS; BUFFERS; CHINCHILLA; DIFFUSION; FALSE POSITIVE REACTIONS; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FLUORESCENT DYES; GLYCEROL; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; PHENYLENEDIAMINES; SPECIMEN HANDLING; SPIRAL GANGLION;

EID: 0029881570     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/19962003398     Document Type: Article

References (5)

1. Barthel, L.K. 1990. Improved method for obtaining 3-micron cryosections for immunocy-tochemistry. J. Histochem. Cytochem. 35:1383-1388.
2. Coons, A.H. and M.H. Kaplan. 1950. Localization of antigen in tissue cells. J. Exp. Med. 97:1-13.
3. Johnson, G.D. and G.M. de C.N. Araujo. 1981. A simple method of reducing the fading of immunofluorescence during microscopy. J. Immunol. Methods 45:349-350.
4. Longin, A., C. Souchier, M. Ffrench and P.A. Bryon. 1993. Comparison of anti-fading agents used in fluorescence microscopy: image analysis and laser confocal microscopy study. J. Histochem. Cytochem. 47:1833-1840.
5. Santi, P.A., S.A. Levine, P. Mancini, P. Menning and D. Swartz. 1994. Inner ear autoantibodies in patients with Memere's disease, p. 471-474. In R. Filipo and M. Barbara (Eds.), Proceedings of the Third International Symposium on Memere's Disease. Kugler Publications, Amsterdam/New York.