Identification and characterization of various differentiate growth plate chondrocytes from porcine by countercurrent centrifugal elutriation

Journal of Cellular Biochemistry

Volumn 60, Issue 4, 1996, Pages 508-520

  Lee, K M ^a,b   Fung, K P ^a   Leung, P C ^a   Leung, K S ^a  

^a CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

^b CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

Alkaline phosphatase; Cartilage; Chondrocyte; Countercurrent centrifugal elutriation; Porcine

Indexed keywords

SUIDAE; ANIMALIA; SUS SCROFA;

ALKALINE PHOSPHATASE;

ANIMAL CELL; ARTICLE; CARTILAGE CELL; CELL DENSITY; CELL DIFFERENTIATION; CELL SIZE; DNA SYNTHESIS; GROWTH PLATE; GROWTH RATE; NONHUMAN; PRIORITY JOURNAL; SWINE;

ALKALINE PHOSPHATASE; ANIMALS; CELL COUNT; CELL DIFFERENTIATION; CELL SEPARATION; CELL SIZE; CELLS, CULTURED; CENTRIFUGATION; COUNTERCURRENT DISTRIBUTION; DNA; GROWTH PLATE; PROTEOGLYCANS; SWINE;

EID: 0029880905     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4644(19960315)60:4<508::AID-JCB7>3.0.CO;2-W     Document Type: Article

References (26)

1. Buckwalter JA, Mower D, Ungar R, Schaaeffer J, Ginsberg B (1986): Morphometric analysis of chondrocyte hypertrophy. J Bone Joint Surg [Am] 68:243-255.
2. de Bernard B, Bianco P, Bonnuci E, Constantini MI, Lunazzi GC, Martinuzzi PI, Modicky C, Moro L, Poolesello P, Stagni N, Vittur F (1986): Biochemical and immunohistochemical evidence that in cartilage an alkaline phosphatase is a Ca2+ binding glycoprotein. J Cell Biol 103:1615-1623
3. Delbruck A, Gurr E (1986): Pathobiochemistry of the chondroycte, J Clin Chem Clin Biochem 24:913-959.
4. Fallon MD, Whyte MP, Teitelbaum SL (1986): Stereospecific inhibition of alkaline phosphatase by L-tetramisole prevents in vitro calcification. Lab Invest 43:489-494.
5. Farley JR, Baylink DJ (1986): Skeletal alkaline phosphatase activity as a bone formation index. Metabolism 35:563-571.
6. Fauran-Clavel MJ, Oustrin J (1986): Alkaline phosphatase and bone calcium parameters. Bone 7:95-99.
7. Grading DJ, Meistrich ML (1980): Separation of solid tumor cell populations. Antibiot Chemother 28:137-141.
8. Grant WT, Sussman MD, Balian G (1985): A disulfide-bonded short chain collagen synthesized by degenerative and calcifying zones of bovine growth plate cartilage. J Biol Chem 260:3798-3803.
9. Gunter TE, Enscik MJ, Puzas JE, Gunter KK, Rosier RN (1990): Cytosolic free calcium concentration in avian growth plate chondrocytes. Cell Calcium 11:445-457.
10. Hunziker EB, Schenk RK, Cruz-Orive LM (1985): Quantitation of chondrocyte performance in growth plate cartilage during longitudinal bone growth. J Bone Joint Surg [Am] 69:162-173.
11. Kuhlman RE (1965): Phosphatases in epiphyseal cartilage: Their possible role in tissue synthesis. J Bone Joint Surg [Am] 47A:545-550
12. Lewinson D, Toister Z, Silbermann M (1982): Quantitative and distributional changes in the activity of alkaline phos-phatase during the maturation of cartilage. J Histochem Cytochem 30:261-269.
13. Lord EM, Keng PC (1984): Methods for using centrifugal elutriation to separate malignant and lymphoid cell populations. J Immunol Methods 68:147-155.
14. O'Keefe RJ, Puzas JE, Brand JS, Rosier EN (1988): Effects of transforming growth factor-B on matrix synthesis by chick growth-plate chondrocytes. Endocrinology 122:2953-2961.
15. O'Keefe RJ, Crab ID, Puzas JE, Rosier RN (1989): Countercurrent centrifugal elutriation: High resolution method for the separation of growth plate chondrocytes. J Bone Joint Surg [Am] 71:607-620.
16. O'Keefe RJ, Rosier RN, Puzas JE (1990): Differential expression of biological effects in maturationally district subpopulations of growth plate chondrocytes. Connect Tissue Res 24:53-66.
17. Prins AP, Lipman JM, Sokoloffk L (1982): Effect of purified growth factors on rabbit articular chondrocytes in monolayer culture: I. DNA synthesis. Arthritis Rheum 25:1217-1227.
18. Puzas JE, Brand JS (1986): The effect of bone cell stimulatory factors can be measured with thymidine incorporation only under specific conditions. Calcif Tissue Int 39: 104-108.
19. Puzas JE, Felter ME (1988): Actions of transforming growth factor β and fibroblast growth factor on bone and cartilage forming cells. In Davidovitch Z (ed): "Biological Mechanisms of Tooth eruptio and root resorption." Birmingham: EBSCO Media, pp 81-86.
20. Ray RL, McLean FM, Low MG, Wuthier RE (1982): Isolation of proliferating chondrocytes from bovine growth plate cartilage by rate zonal centrifugation in ficoll density gradient. J Bone Joint Surg [Am] 64:1221-1224.
21. Register TC, Mclean FM, Low MG, Wuthier RE (1986): Roles of Alkaline phosphatase and labile internal mineral in matrix vesicle-mediated calcification. J Biol Chem 261: 9354-9360.
22. Rosier RN, O'Keefe RJ, Carbb ID, Puzas JE (1989): Transforming growth factor beta: An autocrine regulator of chondrocytes. Connect Tissue Res 20:295-301.
23. Skantze KA, Brinkerhoff CE, Collier JP (1985): Use of agarose culture to measure the effect of transforming growth factor β and epidermal growth factor on rabbit articular chondrocytes. Cancer Res 45:4416-4421.
24. Wier ML, Scott RE (1986): Regulation of the terminal event in cellular differentiation. Biological mechanisms of the loss of proliferative potential. J Cell Biol 102:1955-1964.
25. Wlodarski KH, Reddi AH (1986): Alkaline phosphatase as a marker of osteoinductive cells. Calcif Tissue Int 39:382-385.
26. Wuthier RE (1969): A zonal analysis of inorganic and organic constituents of the epiphysis during endochondral ossification. Calcif Tissue Res 4:20-38.