Analysis of Rous sarcoma virus Gag proteins by mass spectrometry indicates trimming by host exopeptidase

Journal of Virology

Volumn 70, Issue 5, 1996, Pages 3313-3318

  Pepinsky, R Blake ^a   Papayannopoulos, Ioannis A ^a   Campbell, Stephen ^b   Vogt, Volker M ^b  

^a BIOGEN   (United States)

^b Department of Obstetrics Gynecology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GAG PROTEIN; PEPTIDASE;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN PROCESSING; ROUS SARCOMA ONCOVIRUS;

EID: 0029880870     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.5.3313-3318.1996     Document Type: Article

References (34)

1. Bennett, R. P., S. Rhee, R. C. Craven, E. Hunter, and J. W. Wills. 1991 Amino acids encoded downstream of gag are not required by Rous sarcoma virus protease during Gag-mediated assembly. J. Virol 65:272-280.
2. Bhown, A. S., J. C. Bennett, and E. Hunter. 1980 Alignment of the peptides derived from acid-catalyzed cleavage of an aspartylprolyl bond in the major internal structural polypeptide of avian retroviruses. J Biol. Chem. 255:6962-6965.
3. Cameron, C. E., B. Grinde, J. Jentoft, J. Leis, I. T. Weber, T. Copeland, and A. Wlodawer. 1992. Mechanism of inhibition of the retroviral protease by a Rous sarcoma virus peptide substrate representing the cleavage site between the gag p2 and p10 proteins. J. Biol Chem. 267:23735-23741.
4. Campbell, S., and V. Vogt. 1995. Self-assembly in vitro of purified CA-NC proteins from Rous sarcoma virus and human immunodeficiency virus type 1. J. Virol. 69:5487-6497.
5. 4a.Campbell, S., and V. Vogt. Unpublished data
6. Craven, R. C., A. E. Lenre-duPree, C. R. Erdie, C. B. Wilson, and J. W. Wills. 1993 Necessity of the spacer peptide between CA and NC in the Rous sarcoma virus Gag protein. J. Virol. 67:6246-6252
7. Erikson, E., J. Brugge, and R. L. Erikson. 1977 Phosphorylated and non-phosphorylated forms of avian sarcoma virus polypeptide p19. Virology 80:177-185.
8. Henderson, L. E., M. A. Bowers, R. C. Sowder, S. A. Serabyn, D. G. Johnson, J. W. Bess, L. O. Arthur, D. K. Bryant, and C. Fenselau. 1992. Gag proteins of the highly replicative MN strain of human immunodeficiency virus type 1: posttranslational modifications, proteolytic processing, and complete amino acid sequences. J. Virol. 66:1856-1865.
9. Henderson, L. E., T. D. Copeland, R. C. Sowder, A. M. Schultz, and S. Oroszlan. 1988. Analysis of proteins and peptides from sucrose banded HTLV-III, p. 135-147. In D. Bolognesi (ed.). Human retroviruses, cancer and AIDS: approaches to prevention and therapy. Alan R Liss Inc., New York.
10. Hunter, E., J. C. Bennett, A. Bhown, R. B. Pepinsky, and V. M. Vogt. 1983 Amino-terminal amino acid sequence of p10, the fifth major gag polypeptide of avian sarcoma and leukemia viruses. J. Virol. 45:885-888.
11. Johnson, S. P., M. Veigl, T. Vanaman, and J. Leis. 1983 Cyanogen bromide digestion of the avian myeloblastosis virus pp19 protein: isolation of an amino terminal peptide that binds to viral RNA. J. Virol. 45:876-881.
12. Lai, M. M. C. 1976. Phosphoproteins of Rous sarcoma viruses. Virology 74:287-301.
13. Leis, J., and J. Jentoft. 1983. Characteristics and regulation of interaction of avian retrovirus pp12 protein with viral RNA. J. Virol 48:361-369.
14. Leis, J., S. Johnson, L. S. Collins, and J. A. Traugh. 1984. Effects of phosphorylation of avian retrovirus nucleocapsid protein pp12 on binding of viral RNA. J. Biol. Chem. 259:7726-7732.
15. 2-/phospholipid-dependent protein kinase. Eur. J. Biochem. 179:415-422.
16. Mervis, R. J., N. Ahmad, E. P. Lillehoj, M. G. Raum, F. H. R. Salazar, H. W. Chan, and S. Venkatesan. 1988. The gag gene products of human immuno-deficiency virus type 1: alignment within the gag open reading frame, identification of posttranslational modifications, and evidence for alternative gag precursors. J. Virol. 62:3993-4002.
17. Misono, K. S., F. S. Sharief, and J. P. Leis. 1980. Primary structure of Rous sarcoma virus RNA-associated polypeptide pp12. Fed. Proc. Exp. Biol. Abstr. 60:1611.
18. 16a.Nelle, T., and J. Wills. Submitted for publication.
19. Palmiter, R. D., J. Gagnon, V. M. Vogt, S. Ripley, and R. Eisenman. 1978. The NH2-terminal sequence of an avian oncovirus gag precursor polyprotein (Pr76gag). Virology 91:423-433.
20. Pepinsky, R. B. 1983. Ph.D. thesis. Cornell University, Ithaca, N.Y
21. Pepinsky, R. B., R. J. Mattaliano, and V. M. Vogt. 1986. Structure and processing of the p2 region of avian sarcoma and leukemia virus gag precursor polyproteins J Virol. 58:50-58.
22. Pepinsky, R. B., I. A. Papayannopoulos. E. P. Chow, N. K. Krishna, R. C. Craven, and V. M. Vogt. 1995. Differential proteolytic processing leads to multiple forms of the CA protein in avian sarcoma and leukemia viruses. J. Virol. 69:6430-6438.
23. Pepinsky, R. B., and V. M. Vogt. 1983. Purification and properties of a fifth major viral Gag protein from avian sarcoma and leukemia viruses J Virol. 45:648-658.
24. Pepinsky, R. B., and V. M. Vogt. 1984. Fine-structure analyses of lipid-protein and protein-protein interactions of gag protein p19 of the avian sarcoma and leukemia viruses by cyanogen bromide mapping. J Virol. 52:145-153
25. Pettit, S. C. M. D. Moody, R. S. Wehbie, A. H. Kaplan, P. V. Nantermet, C. A. Klein, and R. Swanstrom. 1994. The p2 domain of human immuno-deficiency virus type 1 gag regulates sequential proteolytic processing and is required to produce fully infectious virions. J Virol. 68:8017-8027
26. Sauer, R. T., D. W. Allen, and H. D. Niall. 1981. Amino acid sequence of p15 from avian myeloblastosis virus complex. Biochemistry 20:3784-3791.
27. 24a.Schatz, G., I. Pichova, and V. Vogt. Unpublished data.
28. Schwartz, D., R. Tizard, and W. Gilbert. 1983. Nucleotide sequence of Rous sarcoma virus. Cell 32:853-869.
29. Shealy, D. J., A. G. Mosser, and R. R. Reukert. 1980. Novel p19-related protein in Rous-associated virus type 61: implications for avian gag gene order. J. Virol 34:431-437.
30. Tritch, R. J., Y. E. Cheng, F. H. Yin, and S. Erickson-Viitanen. 1991. Mutagenesis of protease cleavage sites in the human immunodeficiency virus type 1 Gag polyprotein. J. Virol. 65:922-930.
31. Vogt V. M., R. B. Pepinsky, and L. E. Southard. 1985. The primary structure of p19 species of avian sarcoma and leukemia viruses. J. Virol. 56:31-39.
32. Wills, J. W., C. E. Cameron, C. B. Wilson, Y. Xiang, R. P. Bennett, and J. Leis. 1994. An assembly domain of the Rous sarcoma virus Gag protein required late in budding. J Viral. 68:6605-6618.
33. Wills, J. W., R. C. Craven, and J. A. Achacoso. 1989. Creation and expression of myristylated forms of Rous sarcoma virus Gag proteins in mammalian cells J. Virol. 63:4431-4343.
34. Yoshinaka, Y., C. Kirk, and R. B. Luftig. 1980. Aminopeptidase activity associated with purified murine leukemia viruses. J Gen. Virol 46:363-372.