Consecutive cycles of precise, unidirectional 14-bp deletions using a BseRI/BsgI trimming plasmid

BioTechniques

Volumn 20, Issue 3, 1996, Pages 446-451

  Ariazi, Eric A ^a   Gould, Michael N ^a,b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b UNIVERSITY OF WISCONSIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPICILLIN; ANTIBIOTIC AGENT; COMPLEMENTARY DNA; DIHYDROFOLATE REDUCTASE; DNA FRAGMENT; PLASMID DNA; TRIMETHOPRIM;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM CULTURE; BASE PAIRING; DNA SEQUENCE; GENE DELETION; MOLECULAR CLONING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION;

EID: 0029880696     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/19962003446     Document Type: Article

References (4)

1. Hasan, N., S.C. Kim, A.J. Podhajska and W. Szybalski. 1986. A novel multistep method for generating precise unidirectional deletions using BspMI, a class-IIS restriction enzyme. Gene 50:55-62.
2. Hasan, N., J. Kur and W. Szybalski. 1989. An MboII/FokI trimming plasmid allowing consecutive cycles of precise 1- to 12-basepair deletions in cloned DNA. Gene 52:305-311.
3. Szybalski, W., S.C. Kim, N. Hasan and A.J. Podhajska. 1991. Class-IIS restriction enzymes - a review Gene 100:13-26.
4. Yan, Y.C., Y. Bai, L. Wang, S. Miao and S.S. Koide. 1990. Characterization of cDNA encoding a human sperm membrane protein related to A4 amyloid protein. Proc. Natl. Acad. Sci. USA 57:2405-2408.