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Volumn 271, Issue 5257, 1996, Pages 1873-1876

An RNA polymerase II elongation factor encoded by the human ELL gene

Author keywords

[No Author keywords available]

Indexed keywords

ELONGATION FACTOR; RNA POLYMERASE II;

EID: 0029879664     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5257.1873     Document Type: Article
Times cited : (291)

References (27)
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    • Approximately 50 pmol of PLRP-S-punfied rat p80 (6) was reduced, S-carboxyamidomethylated. digested with trypsin, and fractionated by microbore HPLC. Optimal peptides were determined by differential absorbance of ultraviolet light and matrix assisted laser de sorption mass spectrometry (Lasermat; Finnigan-MAT, San Jose, CA). The peptides were then sequenced by automated Edman degradation [W S Lane, A. Galat, M. W. Harding, S. L Schreiber, J. Protein Chem 10, 151 (1991)].
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    • note
    • 4. 1 mM DTT, and 10% (v/v) glycerol After 90 min on ice, the protein was dialyzed for 3 hours at 4°C against the same buffer without DTT
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    • D R Duan et al , Science 269, 1402 (1995); T. Aso, W. S. Lane, J W. Conaway, R. C Conaway, ibid., p 1439, A Kibel, O. lliopoulos, J. A. DeCaprio, W. G Kaelin Jr, ibid., p. 1444.
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    • note
    • We thank R. Robinson of the Harvard Microchemistry Facility for peptide isolation. Supported by NIH grant GM41628 and by funds provided to the Oklahoma Medical Research Foundation by the H. A and Mary K. Chapman Charitable Trust (A.S , R.C.C , and J.W.C) and by the William K. Warren Foundation (K W J) AS is supported by a postdoctoral fellowship from the Jane Coffin Childs Memorial Fund for Medical Research.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.