Surface-associated material from the bacterium Actinobacillus actinomycetemcomitans contains a peptide which, in contrast to lipopolysaccharide, directly stimulates fibroblast interleukin-6 gene transcription

European Journal of Biochemistry

Volumn 236, Issue 3, 1996, Pages 871-876

  Reddi, Krisanavane ^a   Nair, Sean P ^a   White, Peter A ^a   Hodges, Stephen ^a   Tabona, Peter ^a   Meghji, Sajeda ^a   Poole, Stephen ^b   Wilson, Michael ^a   Henderson, Brian ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL   (United Kingdom)

Author keywords

Bacteria; Bacterial surface proteins; Cytokine; Interleukin 6; Lipopolysaccharide

Indexed keywords

CELL SURFACE PROTEIN; DEXAMETHASONE; INTERLEUKIN 1; INTERLEUKIN 6; LIPOPOLYSACCHARIDE; TUMOR NECROSIS FACTOR;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; ARTICLE; CELL SURFACE; CONTROLLED STUDY; CYTOCHEMISTRY; FIBROBLAST; GENETIC TRANSCRIPTION; GINGIVA; GRAM NEGATIVE BACTERIUM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; MONOCYTE; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; ANTIBODIES; BONE RESORPTION; CELL MEMBRANE; CELLS, CULTURED; CYTOKINES; DEXAMETHASONE; FIBROBLASTS; GINGIVA; INTERLEUKIN 1 RECEPTOR ANTAGONIST PROTEIN; INTERLEUKIN-1; INTERLEUKIN-6; KINETICS; LIPOPOLYSACCHARIDES; PEPTIDES; POLYMERASE CHAIN REACTION; RNA, MESSENGER; SIALOGLYCOPROTEINS; TRANSCRIPTION, GENETIC; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0029879640     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.00871.x     Document Type: Article

References (29)

1. Genco, R. J., Goldman, H. M. & Cohen, D. W. (1989) Contemporary periodontics, Mosby, Philadelphia.
2. Liakoni, H. P., Barber, P. & Newman, H. P. (1987) Bacterial penetration of pocket soft tissues in chronic adult and juvenile periodontitis cases. An ultrastructural study. J. Clin. Periodont. 14, 22-28.
3. Socransky, S. S. & Haffajee, A. D. (1991) Microbial mechanisms in the pathogenesis of destructive periodontal diseases: a critical assessment. J. Periodont. Res. 26, 195-212.
4. Zambon, J. J. (1985) Actinobacillus actinomycetemcomitans in human periodontal diseases, J. Clin. Periodont. 12, 1-20.
5. Wilson, M., Kamin, S. & Harvey, W. (1985) Bone-resorbing activity of purified capsular material from Actinobacillus actinomycetemcomitans, J. Periodont. Res. 20, 484-491.
6. Meghji, S., Wilson, M., Barber, P. & Henderson, B. (1994) Bone-resorbing activity of surface-associated material from Actinobacillus actinomycetemcomitans and Eikenella corrodens, J. Med. Microbiol. 41, 197-203.
7. Kirby A. C., Meghji, S., Nair, S. P., White, P., Reddi, K., Nishihara, T., Nakashima, K., Willis, A. C. Sim, R., Wilson, M. & Henderson, B. (1995) The potent bone resorbing mediator of Actinobacillus actinomycetemcomitans is homologous to the molecular chaperone GroEL. J. Clin. Invest. 96, 1185-1194.
8. White, P., Wilson, M. Nair, S. P., Kirby, A. C., Reddi, K. & Henderson, B. (1995) Characterization of an anti-proliferative surface-associated protein from Actinobacillus actinomycetemcomitans which can be neutralized by sera from a proportion of patients with localized juvenile periodontitis, Infect. Immun. 63, 2612-2618.
9. Reddi, K., Wilson, M., Poole, S. & Henderson, B. (1995) Relative cytokine-stimulating activities of surface components of the oral periodontopathic bacterium Actinobacillus actinomycetemcomitans, Cytokine 7, 534-541.
10. Morrison, D. C. & Leive, L. (1975) Isolation and characterization of two fractions of lipopolysaccharide from E. coli 0111:B4, J. Biol. Chem. 250, 2911-2920.
11. Westphal, O. & Jann, K. (1965) Bacterial lipopolysaccharide. Extraction with phenol-water and further applications of the procedure. Methods Carbohyd. Res. 5, 83-91.
12. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the Polin phenol reagent. J. Biol. Chem. 193, 265-275.
13. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. (1956) Colorimetric method for the determination of sugars and related substances, Anal. Chem. 28, 350-356.
14. Bristow, A. F., Mosley, K. & Poole, S. (1991) Interleukin-1β production in vivo and in vitro in rats and mice measured using specific immunoradiometric assays, J. Molec. Endocrinol. 7, 1-7.
15. Nair, S. P., Song, Y., Meghji, S., Reddi, K., Harris, M., Ross, A., Poole, S., Wilson, M. & Henderson, B. (1995) Surface-associated proteins from Staphylococcus aureus demonstrate potent bone-resorbing activity, J. Bone Miner. Res. 10, 726-734.
16. Chomczynski, P. & Sacchi, N. (1987) Single step method of RNA isolation by acid guanidium thiocyanate phenol chloroform extraction. Anal. Biochem. 162, 156-159.
17. Jung, H. C., Eckman, L., Yang, S.-K., Panja, A., Fierer, J., Morzycka-Wroblewska, E. & Kagnoff, M. F. (1995) A distinct array of proinflammatory cytokines is expressed in human colon epithelial cells in response to bacterial invasion. J. Clin. Invest. 95, 55-65.
18. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
19. Henderson, B. & Wilson, M. (1995) Modulins: A new class of cytokine-inducing, pro-inflammatory bacterial virulence factor, Inflammation Res. 44, 187-197.
20. Tosato, G. & Jones, K. D. (1990) Interleukin-1 induces interleukin-6 production in peripheral blood monocytes, Blood 73, 1305-1309.
21. Jirik, F. R., Podor, T. J., Hirano, T., Kishimoto, T., Loskutoff, D. J., Carson, D. A. & Lotz, M. (1989) Bacterial lipopolysaccharide and inflammatory mediators augment IL-6 secretion by human endothelial cells, J. Immunol. 142, 144-147.
22. Littlewood, A. J., Russel, J., Harvey, G. R., Hughes, D. E., Russell, R. G. G. & Gowen, M. (1991) The modulation of the expression of IL-6 and its receptor in human osteoblasts in vitro, Emlocrinol 129, 1513-1520.
23. Van Damme, J., Cayphas, S., Opdenakker, G., Billiau, A. & Van Snick, J. (1987) Interleukin-1 and poly(rI).poly(rC) induce production of a hybridoma growth factor by human fibroblasts, Eur. J. Immunol. 17, 1-7.
24. Sehgal, P. B. (1992) Regulation of IL-6 gene expression, Res. Immunol. 143, 724-734.
25. 4 enhances interleukin-6 (IL-6) production and IL-6 messenger RNA accumulation in human monocytes in vitro: Transcriptional and posttranscriptional mechanisms, Blood 80, 1004-1011.
26. Zhang, Y. Broser, M. & Rom, W. N. (1994) Activation of the interleukin-6 gene by Mycobacterium tuberculosis and lipopolysaccharide is mediated by nuclear factors NF-IL-6 and NF-κB, Proc. Natl Acad. Sci. USA 91, 2225-2229.
27. Scheinman, R. I., Cogswell, P. C., Lofquist, A. K. & Baldwin, A. S. (1995) Role of transcriptional activation of IkBa in mediation of immunosuppression by glucocorticoids, Science 270, 283-286.
28. Auphan, N., Didonuto, J. A., Rosette, C., Helmberg, A. & Karin, M. (1995) Immunosuppression by glucocorticoids: Inhibition of NF-κB activity through induction of Iκ-B synthesis, Science 270, 286-290.
29. Gonzalez-Pastor, J. E., San Millan, J. L. & Moreno, F. (1994) The smallest known gene, Nature 369, 281.