Optimization of competitor poly (dI-dC) · poly (dI-dC) levels is advised in DNA-protein interaction studies involving enriched nuclear proteins

BioTechniques

Volumn 20, Issue 3, 1996, Pages 439-444

  Larouche, Kathy ^a   Bergeron, Marie Josée ^a   Leclerc, Steeve ^a   Guérin, Sylvain L ^a  

^a LAVAL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DNA BINDING PROTEIN; NUCLEAR PROTEIN; OLIGONUCLEOTIDE; POLYMER;

ANIMAL CELL; ARTICLE; COMPETITIVE INHIBITION; CORNEA EPITHELIUM; DNA BINDING; DNA SEQUENCE; ELECTROPHORETIC MOBILITY; EPITHELIUM CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DNA INTERACTION; RABBIT;

ANIMALS; BASE SEQUENCE; BINDING, COMPETITIVE; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; POLYDEOXYRIBONUCLEOTIDES; PROTEIN BINDING; RABBITS; SP1 TRANSCRIPTION FACTOR;

EID: 0029879410     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/19962003439     Document Type: Article

References (19)

1. Audet, J.-F., J.-Y. Masson, G.D. Rosen, C. Salesse and S.L. Guérin. 1994. Multiple regulatory elements control the basal promoter activity of the human α4 integrin gene. DNA Cell Biol. 13:1071-1085.
2. Ausubel, F.M., B. Roger, R.E. Kingston, D.D. Moore, J.G. Seideman, J.A. Smith and K. Struhl. 1987. Current Protocols in Molecular Biology. Greene Publishing Associates and Wiley-Interscience, New York.
3. Benson, G.V., T.-H.E. Nguyen and R.L. Maas. 1995. The expression pattern of the murine Hoxa-10 gene and the sequence recognition of its homeodomain reveal specific properties of abdominal B-like genes. Mol. Cell. Biol. 15:1591-1601.
4. Boisjoly, H.M., C. Laplante, S.F. Bernatchez, C. Salesse, M. Giasson and M.-C. Joly. 1993. Effects of EGF, IL-1 and their combination on in vitro corneal epithelial wound closure and cell chemotaxis. Exp. Eye Res. 57:293-300.
5. De Vries, E., W. Van Driel, S.J.L. Van den Heuvel and P.C. Van der Vliet. 1987. Contact analysis of HeLa nuclear factor 1 recognition site reveal symmetrical binding at one site of the DNA helix. EMBO J. 6:161-168.
6. Dynan, W.S. and R. Tjian. 1983. The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter. Cell 35:79-87.
7. Harvey, M., I. Brisson and S.L. Guérin. 1993 A simple apparatus for fast and inexpensive recovery of DNA from polyacrylamide gels. BioTechniques 14:942-947.
8. Ossipow, V., U.K. Laemmli and U. Schibler. 1993. A simple method to renature DNA-binding proteins separated by SDS/polyacrylarmide gel electrophoresis. Nucleic Acids Res. 27:6040-6041.
9. Ren, L., H. Chen and E.A. Sternberg. 1994. Tethered bandshift assay and affinity purification of a new DNA-binding protein. BioTechniques 15:852-855.
10. Robidoux, S., W. Eskild, C.F. Kroepelin, V. Hansson and S.L. Guérin. 1992. Salt-dependent formation of DNA-protein complexes in vitro, as viewed by the gel mobility shift assay. BioTechniques 13:354-358.
11. Roy, R., P. Gosselin and S.L. Guérin. 1991. A short protocol for micro-purification of nuclear proteins from whole animal tissue. BioTechniques 11:770-777.
12. Roy, R.J., P. Gosselin, M.J. Anzivino, D.D. Moore and S.L. Guérin. 1992. Binding of a nuclear protein to rat growth hormone silencer element. Nucleic Acids Res. 20:401-408.
13. Saisanit, S. and X.-H. Sun. 1995. A novel enhancer, the pro-B enhancer, regulates Id1 gene expression in progenitor B cells. Mol. Cell. Biol. 15:1513-1521.
14. Schneider, R., T. Dorper, I. Gander, R. Mertz and E.L. Winnacker. 1986. A sensitive and rapid gel retention assay for nuclear factor I and other DNA-binding proteins in crude nuclear extracts. Nucleic Acids Res. 14:1303-1317.
15. Singh, H., R. Sen, D. Baltimore and P.A. Sharp. 1986. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. Nature 319:145-158.
16. Stevens, S.Y., P.C. Swanson and G.D. Glick. 1995. Application of the gel shift assay to study the affinity and specificity of anti-DNA autoantibodies. J. Immunol. Methods 177.1-2.
17. Timchenko, N., D.R. Wilson, L.R. Taylor, S. Abdelsayed, M. Wilde, M. Sawadogo and G.J. Darlington. 1995. Autoregulation of the human C/EBPα gene by stimulation of upstream stimulatory factor binding. Mol. Cell. Biol. 15:1192-1202.
18. 33P-labeling in the electrophoretic mobility shift assay BioTechniques 16:590-592.
19. Wu, R.-L., T.-T. Chen and T.-T. Sun. 1994. Functional importance of an Sp1- and NFkB-related nuclear protein in a keratinocyte-specific promoter of rabbit K3 keratin gene. J. Biol. Chem. 11:28450-28459