Poliovirus RNA polymerase mutation 3D-M394T results in a temperature-sensitive defect in RNA synthesis

Virology

Volumn 217, Issue 2, 1996, Pages 459-469

  Barton, David J ^a   Morasco, B Joan ^a   Eisner Smerage, Lucia ^a   Collis, Phillip S ^a   Diamond, Scott E ^b   Hewlett, Martinez J ^c   Merchant, Mary A ^a   O'Donnell, Brian J ^a   Flanegan, James B ^a  

^a UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF COLORADO   (United States)

^c UNIVERSITY OF ARIZONA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE;

ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; NONHUMAN; POLIOMYELITIS VIRUS; PRIORITY JOURNAL; RNA SYNTHESIS; TEMPERATURE SENSITIVE MUTANT; TEMPERATURE SENSITIVITY; VIRUS MUTANT; VIRUS REPLICATION;

EID: 0029878863     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.1996.0140     Document Type: Article

References (42)

1. Agut, H., Kean, K. M., Fichot, O., Morasco, J., Flanegan, J. B., and Girard, M. (1989). A point mutation in the poliovirus polymerase gene determines a complementable temperature-sensitive defect of RNA replication. Virology 168, 302-311.
2. Barton, D. J., and Flanegan, J. B. (1993). Coupled translation and replication of poliovirus RNA in vitro: Synthesis of functional 3D polymerase and infectious virus. J. Virol. 67, 822-831.
3. Barton, D. J., Black, E. P., and Flanegan, J. B. (1995). Complete replication of poliovirus in vitro: Preinitiation RNA replication complexes require soluble cellular factors for the synthesis of VPg-linked RNA. J. Virol. 69, 5516-5527.
4. Bernstein, H. D., Sarnow, P., and Baltimore, D. (1986). Genetic complementation among poliovirus mutants derived from an infectious cDNA clone. J. Virol. 60, 1040-1049.
5. pol on RNA polymerase activity and on polyprotein cleavage. J. Virol. 63, 4866-4874.
6. Burns, C. C., Richards, O. C., and Ehrenfeld, E. (1992). Temperature-sensitive polioviruses containing mutations in RNA polymerase. Virology 189, 568-582.
7. Charini, W. A., Burns, C. C., Ehrenfeld, E., and Semler, B. L. (1991). Trans rescue of a mutant poliovirus RNA polymerase function. J. Virol. 65, 2655-2665.
8. Delarue, M., Poch, O., Tordo, N., Moras, D., and Argos, P. (1990). An attempt to unify the structure of polymerases. Protein Eng. 3, 461-467.
9. Diamond, S. E., and Kirkegaard, K. (1994). Clustered charged-to-alanine mutagenesis of poliovirus RNA-dependent RNA polymerase yields multiple temperature-sensitive mutants defective in RNA synthesis. J. Virol. 68, 863-876.
10. Flanegan, J. B., Petterson, R. F., Ambros, V., Hewlett, N. J., and Baltimore, D. (1977). Covalent linkage of a protein to a defined nucleotide sequence at the 5′-terminus of virion and replicative intermediate RNAs of poliovirus. Proc. Natl. Acad. Sci. USA 74, 961-965.
11. Flanegan, J. B., and Baltimore, D. (1979). Poliovirus polyuridylic acid polymerase and RNA replicase have the same viral polypeptide. J. Virol. 29, 352-360.
12. Flanegan, J. B., and Van Dyke, T. A. (1979). Isolation of a soluble and template-dependent poliovirus RNA polymerase that copies virion RNA in vitro. J. Virol. 32, 155-161.
13. Harris, K. S., Hellen, C. U. T., and Wimmer, E. (1990). Proteolytic processing in the replication of picornaviruses. Semin. Virol. 1, 323-333.
14. Harris, K. S., Reddigari, S. R., Nicklin, M. J. H., Hammerle, T., and Wimmer, E. (1992). Purification and characterization of poliovirus polypeptide 3CD, a proteinase and a precursor for RNA polymerase. J. Virol. 66, 7481-7489.
15. Hewlett, M. J., Axelrod, J. H., Antinoro, N., and Feld, R. (1982). Isolation and preliminary characterization of temperature-sensitive mutants of poliovirus type 1. J. Virol. 41, 1089-1094.
16. Jablonski, S. A., and Morrow, C. D. (1993). Enzymatic activity of poliovirus RNA polymerases with mutations at the tyrosine residue of the conserved YGDD motif: Isolation and characterization of polioviruses containing RNA polymerases with FGDD and MGDD sequences. J. Virol. 67, 373-381.
17. Kaplan, G., Levy, A., and Racaniello, V. R. (1989). Isolation and characterization of HeLa cell lines blocked at different steps in the poliovirus life cycle. J. Virol. 63, 43-51.
18. Kitamura, N., Semler, B. L., Rothberg, P. G., Larsen, G. R., Adler, C. J., Dorner, A. J., Emini, E. A., Hanecak, R., Lee, J. J., van der Werf, S., Anderson, C. W., and Wimmer, E. (1981). Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature 291, 547-553.
19. Kuchta, R. D., Benkovic, P., and Benkovic, S. J. (1988). Kinetic mechanism whereby DNA polymerase I (Klenow) replicates DNA with high fidelity. Biochemistry 27, 6716-6725.
20. Kunkel, T. A. (1985). Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82, 488-492.
21. Lee, Y. F., Nomoto, A., Detjen, B. M., and Wimmer, E. (1977). A protein covalently linked to poliovirus genome RNA. Proc. Natl. Acad. Sci. USA 74, 59-63.
22. Molla, A., Paul, A. V., and Wimmer, E. (1991). Cell-free, de novo synthesis of poliovirus. Science 254, 1647-1651.
23. Molla, A., Paul, A. V., and Wimmer, E. (1993). Effects of temperature and lipophilic agents on poliovirus formation and RNA synthesis in a cell-free system. J. Virol. 67, 5932-5938.
24. Neufeld, K. L., Richards, O. C., and Ehrenfeld, E. (1991a). Purification, characterization, and comparison of poliovirus RNA polymerase from native and recombinant sources. J. Biol. Chem. 266, 24212-24219.
25. pol in recombinant baculovirus-infected Spodoptera frugiperda cells. Virus Res. 19, 173-188.
26. Nomoto, A., Kitamura, N., Golini, F., and Wimmer, E. (1977). The 5′-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg. Proc. Natl. Acad. Sci. USA 74, 5345-5349.
27. Novak, J. E., and Kirkegaard, K. (1994). Coupling between genome translation and replication in an RNA virus. Genes Dev. 8, 1726-1737.
28. Plotch, S. J., Palant, Q., and Gluzman, Y. (1989). Purification and properties of poliovirus RNA polymerase expressed in Escherichia coli. J. Virol. 63, 216-225.
29. Poch, Q., Sauveget, I., Delarue, M., and Tordo, N. (1989). Identification of four conserved motifs among the RNA-dependent polymerase encoding elements. EMBO J. 8, 3867-3874.
30. Racaniello, V. R., and Baltimore, D. (1981). Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome. Proc. Natl. Acad. Sci. USA 78, 4887-4891.
31. Richards, O. C., Ivanoff, L. A., Bienkowska-Szewczyk, K., Butt, B., Petteway, S. R., Jr., Rothstein, M. A., and Ehrenfeld, E. (1987). Formation of poliovirus RNA polymerase 3D in Escherichia coli by cleavage of fusion proteins expressed from cloned viral cDNA. Virology 161, 348-356.
32. Rothstein, M. A., Richards, O. C., Amin, C., and Ehrenfeld, E. (1988). Enzymatic activity of poliovirus RNA polymerase synthesized in Escherichia coli from viral cDNA. Virology 164, 301-308.
33. Sanger, F., Nicklin, S., and Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
34. Shiroki, K., Kato, H., Koike, S., Odaka, T., and Nomoto, A. (1993). Temperature-sensitive mouse cell factors for strand-specific initiation of poliovirus RNA synthesis. J. Virol. 67, 3989-3996.
35. Toyoda, H., Yang, C. F., Takeda, N., Nomoto, A., and Wimmer, E. (1987). Analysis of RNA synthesis of type 1 poliovirus by using an in vitro molecular genetic approach. J. Virol. 61, 2816-2822.
36. Tuschall, D. M., Hiebert, E., and Flanegan, J. B. (1982). Poliovirus RNA-dependent RNA polymerase synthesizes full-length copies of poliovirion RNA, cellular mRNA, and several plant virus RNAs in vitro. J. Virol. 44, 209-216.
37. Van Dyke, T. A., and Flanegan, J. B. (1980). Identification of poliovirus polypeptide P63 as a soluble RNA-dependent RNA polymerase. J. Virol. 35, 732-740.
38. Van Dyke, T. A., Rickles, R. J., and Flanegan, J. B. (1982). Genome-length copies of poliovirion RNA are synthesized in vitro by the poliovirus RNA-dependent RNA polymerase. J. Biol. Chem. 257, 4610-4617.
39. Villa-Komaroff, L., McDowell, M., Baltimore, D., and Lodish, H. F. (1974). Translation of reovirus mRNA, poliovirus RNA and bacteriophage Qb RNA in cell-free extracts of mammalian cells. Methods Enzymol. 30, 709-723.
40. Young, D. C., Tuschall, D. M., and Flanegan, J. B. (1985). Poliovirus RNA-dependent RNA polymerase and host cell protein synthesize product RNA twice the size of poliovirion RNA in vitro. J. Virol. 54, 256-264.
41. Young, D. C., Dunn, B. M., Tobin, G. J., and Flanegan, J. B. (1986). Anti-VPg antibody precipitation of product RNA synthesized in vitro by the poliovirus polymerase and host factor is mediated by VPg on the poliovirion RNA template. J. Virol. 58, 715-723.
42. Young, D. C., Tobin, G. J., and Flanegan, J. B. (1987). Characterization of product RNAs synthesized in vitro by poliovirus RNA polymerase purified by chromatography on hydroxylapatite or poly(U) Sepharose. J. Virol. 61, 611-614.