Culture Maintenance of Isolated Adult Porcine Pancreatic Islets in Three-Dimensional Gel Matrices: Morphologic and Functional Results

Pancreas

Volumn 12, Issue 3, 1996, Pages 221-229

  Falorni, Alberto ^a   Basta, Giuseppe ^a   Santeusanio, Fausto ^a   Brunetti, Paolo ^a   Calafiore, Riccardo ^a  

^a UNIVERSITY OF PERUGIA   (Italy)

Author keywords

Cell storage; Culture maintenance; In vitro; Insulin; Pancreatic islets

Indexed keywords

ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL ISOLATION; CELL STRUCTURE; CELL VIABILITY; DIABETES MELLITUS; EXTRACELLULAR MATRIX; INSULIN RELEASE; NONHUMAN; PANCREAS ISLET; PANCREAS ISLET TRANSPLANTATION; PANCREAS SECRETION; PRIORITY JOURNAL; SWINE;

ANALYSIS OF VARIANCE; ANIMALS; CELL COUNT; CELL SURVIVAL; CELLS, CULTURED; FEMALE; HYDROGEL; ISLETS OF LANGERHANS; PERFUSION; POLYETHYLENE GLYCOLS; RANDOM ALLOCATION; SWINE;

EID: 0029878301     PISSN: 08853177     EISSN: None     Source Type: Journal    
DOI: 10.1097/00006676-199604000-00003     Document Type: Article

References (20)

1. Calafiore R, Calcinaro F, Basta G, et al. A method for the massive separation of highly purified adult porcine islets of Langherhans. Metabolism 1990;39:175-81.
2. Ricordi C, Finke EH, Lacy PE. A method for the mass isolation of islets from the adult pig pancreas. Diabetes 1986; 35:649-53.
3. Ricordi C, Socci C, Davalli AM, et al. Isolation of the elusive pig islets. Surgery 1990;107:688-94.
4. Marchetti P, Finke EH, Gerasimidi-Vazeou A, Falqui L, Scharp DW, Lacy PE. Automated large-scale isolation, in vitro function and xenotransplantation of porcine islets of Langherhans. Transplantation 1991;52:209-13.
5. Van Dejinen JHM, Hulstaert CE, Wolters GHJ, Van Schilfgaarde R. Significance of the peninsular extracellular matrix for islet isolation from the pancreas of rat, dog, pig and man. Cell Tissue Res 1992;267:139-42.
6. Lim R, Sun AM. Microencapsulated islets as a bioartificial pancreas. Science 1990;210:908-10.
7. Calafiore R, Calcinaro F, Basta G, et al. Microencapsulated mammalian cells as therapeutic products. In: Prescott LF, Nimmo WS, eds. Novel drug delivery and its therapeutic application. New York: John Wiley & Sons, 1989: 305-12.
8. Calafiore R, Basta G, Falorni A, Calcinaro F, Pietropaolo M, Brunetti P. A method for the large-scale production of microencapsulated islets: in vitro and in vivo results. Diabetes Nutr Metab 1992;5:23-9.
9. Calafiore R, Basta G, Falorni A, et al. Vascular graft of microencapsulated human pancreatic islets in non immunosuppressed diabetic recipients: preliminary results. Diabetes Nutr Metab 1991;4:45-8.
10. Lacy PE, Hegre OD, Gerasimidi-Vazeou A, Gentile FT, Dionne KE. Maintenance of normoglycemia in diabetic mice by subcutaneous xenografts of encapsulated islets. Science 1991;254:1782-4.
11. Lanza RP, Butler DH, Borland KM, et al. Xenotransplantation of canine, bovine, and porcine islets in diabetic rats without immunosuppression. Proc Natl Acad Sci USA 1991; 88:11100-4.
12. Gray DWR, Morris PJ. The use of fluorescein diacetate and ethidium bromide as a viability stain for isolated islets of Langherhans. Stain Technol 1987;62(6):373-81.
13. United States Pharmacopeia. Steril Test Methods 1990; XXII:1483-8.
14. Latif ZA, Noel J, Alejandro R. A simple method of staining fresh and cultured islets. Transplantation 1987;45(4):827-30.
15. Crowther NJ, Gotfredsen CF, Moody AJ, Green IC. Porcine islet isolation, cellular composition and secretory response. Horm Metab Res 1989;21:590-4.
16. Pipeleers DG. Heterogeneity in pancreatic beta-cell population. Diabetes 1992;41:777-82.
17. Pipeleers DG. Glucose-induced insulin release depends on functional cooperation between islet cells. Proc Natl Acad Sci USA 1992;79:1322-5.
18. Pipeleers DG, Schuit FC. Interplay of nutrients and hormones in the regulation of insulin release. Endocrinology 1985;117:824-8.
19. Unger RH. Diabetic hyperglicaemia: link to impaired glucose transport in pancreatic beta-cells. Science 1991;251: 1200.
20. Liang Y, Najafi H, Smith RM, et al. Concordant glucose induction of glucokinase, glucose usage, and glucose-stimulated insulin release in pancreatic islets maintained in organ culture. Diabetes 1992;41:792-5.