Application of high-performance liquid chromatography-electrospray ionization mass spectrometry and matrix-assisted laser-desorption ionization time-of-flight mass spectrometry in combination with selective enzymatic modifications in the characterization of glycosylation patterns in single-chain plasminogen activator

Journal of Chromatography A

Volumn 732, Issue 1, 1996, Pages 27-42

  Apffel, Alex ^a   Chakel, John A ^a   Hancock, William S ^a   Souders, Carrie ^b   M'Timkulu, Thabiso ^b   Pungor Jr , Erno ^b  

^a HEWLETT PACKARD LABORATORIES   (United States)

^b BAYER AG   (Germany)

Author keywords

Enzymes; Glycoproteins; Glycosylation; Pharmaceutical analysis

Indexed keywords

GLYCOPROTEIN; RECOMBINANT PLASMINOGEN ACTIVATOR; RECOMBINANT PROTEIN;

ARTICLE; CARBOHYDRATE ANALYSIS; CONTROLLED STUDY; DRUG MANUFACTURE; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; REACTION ANALYSIS;

AMIDOHYDROLASES; AMINO ACID SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLYCOSYLATION; MASS SPECTROMETRY; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; PLASMINOGEN ACTIVATORS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 0029878124     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/0021-9673(95)01231-1     Document Type: Article

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