Connexin-26 and connexin-43 are differentially expressed and regulated in the rat myometrium throughout late pregnancy and with the onset of labor

Endocrinology

Volumn 137, Issue 5, 1996, Pages 1545-1553

  Orsino, Angela ^a,b,d   Taylor, Carolyn V ^a,b,e   Lye, Stephen J ^a,b,c  

^a MOUNT SINAI HOSPITAL   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c ONTARIO MINISTRY OF HEALTH AND LONG TERM CARE   (Canada)

^d Natl Hlth R and D Program   (Canada)

^e Genesis Research Foundations

Author keywords

[No Author keywords available]

Indexed keywords

CONNEXIN 26; CONNEXIN 43; ESTRADIOL; GAP JUNCTION PROTEIN; PROGESTERONE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL COMMUNICATION; CONTROLLED STUDY; ESTRADIOL BLOOD LEVEL; FEMALE; GENE EXPRESSION REGULATION; LABOR; LABOR INDUCTION; MYOMETRIUM; NONHUMAN; NORTHERN BLOTTING; OVARIECTOMY; PREMATURE LABOR; PRIORITY JOURNAL; PROGESTERONE BLOOD LEVEL; PROTEIN LOCALIZATION; RAT; THIRD TRIMESTER PREGNANCY;

EID: 0029877670     PISSN: 00137227     EISSN: None     Source Type: Journal    
DOI: 10.1210/endo.137.5.8612484     Document Type: Article

References (24)

1. Challis JRG, Lye SJ 1994 Parturition. In: Knobil E, Neill JD (eds) The Physiology of Reproduction, ed 2. Raven Press, New York, pp 985-1032
2. Garfield RE, Sims SM, Daniel EE 1977 Gap junctions: their presence and necessity in myometrium during parturition. Science 198:958-960
3. Garfield RE, Sims SM, Kannan MS, Daniel EE 1978 The possible role of gap junctions in activation of the myometrium during parturition. Am J Physiol 235:C168-C179
4. Beyer EC 1993 Gap junctions. In: Friedlander M, Muecklen M (eds) International Review of Cytology: A Survey of Cell Biology. Academic Press, vol 137C:1-27
5. Paul DL 1986 Molecular cloning of cDNA for rat liver gap junction protein. J Cell Biol 103:123-134
6. Beyer EC, Paul DL, Goodenough DA 1987 Connexin43: a protein from rat heart homologous to a gap junction protein from liver. J Cell Biol 105:2621-2629
7. Risek B, Guthrie S, Kumar N, Gilula NB 1990 Modulation of gap junction transcript and protein during pregnancy in the rat. J Cell Biol 110:269-282
8. Winterhager E, Stutenkemper R, Traub O, Beyer E, Willecke K 1991 Expression of different connexin genes in rat uterus during decidualization and at term. Eur J Cell Biol 55:133-142
9. Lye SJ, Nicholson BJ, Mascarenhas M, MacKenzie L, Petrocelli T 1993 Increased expression of connexin-43 in the rat myometrium during labour is associated with an increase in plasma estrogen: progesterone ratio. Endocrinology 132:2380-2386
10. Petrocelli T, Lye SJ 1993 Regulation of transcripts encoding the myometrial gap junction protein, connexin-43, by estrogen and progesterone. Endocrinology 133:284-282
11. Chow L, Lye SJ 1994 Expression of the gap junction protein connexin-43 is increased in the human myometrium toward term and with the onset of labour. Am J Obstet Gynecol 170:788-795
12. McNutt CM, Nicholson BJ, Lye SJ 1994 ACTH-induced preterm labour in the ewe is associated with increased mRNA and protein levels of myometrial gap junction protein, connexin-43. J Endocrinol 141:195-202
13. Nicholson B, Dermietzel R, Teplow D, Traub O, Willecke K, Revel JP 1987 Two homologous protein components of hepatic gap junctions. Nature 329:732-734
14. Dermietzel R, Spray DC 1993 Gap junctions in the brain: where, what type, how many and why? Trends Neurosci 16:186-192
15. Winterhager E, Brummer F, Dermietzel R, Husler DF, Denker HW 1988 Gap junction formation in rabbit uterine epithelium in response to embryo recognition. Dev Biol 126:203-211
16. Chomczynski P, Sacchi N 1987 Single-step method of RNA isolation by guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159
17. Bradford MM 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
18. Laemmli UK 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
19. Grummer R, Chwalisz K, Mulholland J, Traub O, Winterhager E 1994 Regulation of connexin26 and connexin43 expression in rat endometrium by ovarian steroid hormones. Biol Reprod 51:1109-1116
20. Risek B, Klier G, Phillips A, Hahn DW, Gilula NB 1995 Gap junction regulation in the uterus and ovaries of immature rats by estrogen and progesterone. J Cell Sci 108:1017-1032
21. Dahl G, Berger W 1978 Nexus formation in the myometrium during parturition and induced by estrogen. Cell Biol Int Rep 2:381-387
22. Hotz-Wagenblatt A, Shalloway D 1993 Gap junctional communication and neoplastic transformation. Crit Rev Oncog 4:541-558
23. Winterhager E, Grummer R, Jahn E, Willecke K, Traub O 1993 Spatial and temporal expression of connexin26 and connexin43 in rat endometrium during trophoblast invasion. Dev Biol 157:399-409
24. Goodenough DA, Musil LS 1993 Gap junctions and tissue business: problems and strategies for developing specific functional reagents. J Cell Sci [Suppl] 17:133-138