Cytochrome P450s of isolated rat hepatocytes in spheroid and monolayer cultures

Research Communications in Molecular Pathology and Pharmacology

Volumn 91, Issue 3, 1996, Pages 372-378

  Niwa, Toshiro ^a,d   Koide, Norio ^b   Tsuji, Takao ^b   Imaoka, Susumu ^c   Ishibashi, Fumihide ^a   Funae, Yoshihiko ^c   Katagiri, Masanao ^a  

^a OSAKA KYOIKU UNIVERSITY   (Japan)

^b OKAYAMA UNIVERSITY MEDICAL SCHOOL   (Japan)

^c OSAKA CITY UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^d ASTELLAS PHARMA INC   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450; CYTOCHROME P450 ISOENZYME; CYTOCHROME P450 REDUCTASE; DRUG METABOLIZING ENZYME;

ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STABILITY; LIVER CELL CULTURE; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; SPHEROID CELL;

ANIMALS; CELLS, CULTURED; CYTOCHROME P-450 ENZYME SYSTEM; LIVER; MALE; MICROSOMES, LIVER; NADPH-FERRIHEMOPROTEIN REDUCTASE; RATS; RATS, SPRAGUE-DAWLEY;

EID: 0029876959     PISSN: 10780297     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Diaz, D., Fabre, L, Daujat, M., Aubert, B.S., Bones, R, Michel, H., and Maurel, P. (1990). Omeprazol is an aryl hydrocarbon-like inducer of human hepatic cytochrome P450. Gastroenterology, 99, 739-747.
2. Funae, Y., and Imaoka, S. (1993). Cytochrome P450 in rodents. In cytochrome P450. Edited by J.B. Scenkman and H. Greim (Berlin, Springer-Verlag). 221-238.
3. Holme, J.A., Sonderlind, E., and Dybing, E.(1983). Drug metabolism activities of isolated rat hepatocytes in monolayer culture. Ada Pharmacol. Toxicol., 52, 348-356.
4. Imaoka, S., Terano, Y., and Funae, Y. (1990). Changes in the amount of cytochrome P450s in rat hepatic microsomes with starvation. Arch. Biochem. Biophys., 278, 168-178.
5. Katagiri, M., Sugiyama, T., Tsutsukawa, N., Ishiguro, H., Miyoshi, N., Ishibashi, F., and Taniguchi, N. (1989). Comparative studies on microsomal NADPH-cytochrome P-450 reductase using a monoclonal antibody: Tissue distribution, specific activity and peptide mapping. Int. J. Biochem. 21, 1395-1405.
6. Koide, N., Shinji, T., Tanabe, T., Asano, K., Kawaguchi, M., Sakaguchi, K., Koide, Y., Mon, M., and Tsuji, T. (1989). Continued high albumin production by multicellular spheroids of adult rat hepatocytes formed in the presence of liver-derived proteoglycans. Biochem. Biophys. Res. Commun., 30, 385-391.
7. Koide, N., Sakaguchi, K., Koide, Y., Asano, K., Kawaguchi, M., Matsushima, H., Takenami, T., Shinji, T., Mori, M., and Tsuji, T. (1990). Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments. Exp. Cell Res., 186, 227-235.
8. Namiki, M., Degawa, M., Masuko, T., and Hashimoto, Y. (1989). Changes in the quantity and activity of cytochrome P-450 isozymes in primary cultured rat hepatocytes. Jpn. J. Cancer Res., 80, 126-131.
9. Newman, S.L., Barwick, J.L. Elshoubagy, N.A., and Guzelian, P.S. (1982). Measurement of the metabolism of cytochrome P-450 in cultured hepatocytes by a quantitative and specific immunochemical methods. Biochem. J., 204, 281-290.
10. Nishibe, Y., and Hirata, M. (1993). Effect of phenobarbital and other model induces on cytochrome P450 isoenzymes in primary culture of dog hepatocytes. Xenobiotica, 23, 681-692.
11. Nishibe, Y., and Hirata, M. (1995). Induction of cytochrome P450 isoenzymes in cultured monkey hepatocytes. Int. J. Biochem. Cell Biol., 27, 279-285.
12. Omura, T., and Sato, R. (1964). The carbon monoxide-binding pigment of liver microsomes. J. Biol. Chem., 239, 2370-2378.
13. Paine, A.J. (1990). The maintenance of cytochrome P-450 in rat hepatocyte culture: Some applications of liver cell cultures to the study of drug metabolism, toxicity and the induction of the P-450 system. Chem.-Biol. Interact., 74, 1-31.
14. Shinji, T., Koide, N., and Tsuji, T. (1988). Glycosaminoglycans partially substitute for proteoglycans in spheroid formation of adult rat hepatocytes in primary culture. Cell Struct. Fund., 13, 179-188.
15. Skett, P. (1994). Problems in using isolated and cultured hepatocytes for xenobiotic metabolism/metabolism-based toxicology testing-solutions? Toxic. in Vitro, 8, 491-504.
16. Warren, M., and Fry, J.R.(1988). Influence of medium composition on 7-alkoxycoumarin O-dealkylase activities of rat hepatocytes in primary maintenance culture. Xenobiotica, 18, 973-981.