Generating antibodies against secreted proteins using vascular smooth muscle cells transduced with replication-defective retrovirus

BioTechniques

Volumn 20, Issue 4, 1996, Pages 694-701

  Forough, R ^a,b   Hasenstab, D ^a   Koyama, N ^a   Lea, H ^a   Clowes, M ^a   Clowes, A W ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGENASE; COMPLEMENTARY DNA; PLATELET DERIVED GROWTH FACTOR BB; POLYCLONAL ANTIBODY; TISSUE INHIBITOR OF METALLOPROTEINASE; VIRUS VECTOR;

ANIMAL CELL; ANTIBODY PRODUCTION; ANTIBODY TITER; ARTICLE; BABOON; CAROTID ARTERY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE TRANSFER; MALE; MOLECULAR CLONING; NONHUMAN; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION; PROTEIN SECRETION; RAT; RETROVIRUS; VASCULAR SMOOTH MUSCLE;

ANIMALS; ANTIBODIES; ANTIBODY FORMATION; ARTERIES; BASE SEQUENCE; BLOTTING, NORTHERN; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENETIC VECTORS; GLYCOPROTEINS; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; MALE; MOLECULAR SEQUENCE DATA; MUSCLE, SMOOTH, VASCULAR; POLYMERASE CHAIN REACTION; PROTEASE INHIBITORS; RATS; RATS, INBRED F344; REPLICATION ORIGIN; RETROVIRIDAE; TISSUE INHIBITOR OF METALLOPROTEINASES; TRANSDUCTION, GENETIC;

ANIMALIA; PAPIO HAMADRYAS; UNIDENTIFIED RETROVIRUS;

EID: 0029876690     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Albini, A., Y. Iwamoto, H.K. Kleinman, G.R. Martin, S.A. Aaronson, J.M. Kozlowski and R.N. McEwan. 1987. A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res. 47:3239-3245.
2. Carmichael, D.F., A. Sommer, R.C. Thompson, D.C. Anderson, C.G. Smith, H.G. Welgus and G.P. Stricklin. 1986. Primary structure and cDNA cloning of human fibroblast collagenase inhibitor. Proc. Natl. Acad. Sci. USA 83:2407-2411.
3. Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
4. Church, G.M. and W. Gilbert. 1984. Genomic sequencing. Proc. Natl. Acad. Sci. USA 87:1991-1995.
5. Clowes, A.W., M.A. Reidy and M.M. Clowes. 1983. Kinetics of cellular proliferation after arterial injury. I. Smooth muscle growth in the absence of endothelium. Lab. Invest. 49:327-333
6. Clowes, M.M., C.M. Lynch, A.D. Miller, D.G. Miller, W.R.A. Osborne and A.W. Clowes. 1994. Long-term biological response of injured rat carotid artery seeded with smooth muscle cells expressing refrovirally introduced human genes. J. Clin. Invest. 93:644-651.
7. De Clerck, Y.A. 1988. Purification and characterization of a collagenase inhibitor produced by bovine vascular smooth muscle cells. Arch. Biochem. Biophys. 265:28-37.
8. Fynan, E.F., R.G. Webster, D.H. Fuller, J.R. Haynes, J.C. Santoro and H.L. Robinson. 1993. DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations. Proc. Natl. Acad. Sci. USA 90:11478-11482.
9. Gavrilovic, J., R.M. Hembry, J.J. Reynolds and G. Murphy. 1987. Tissue inhibitor of metalloproteinases (TIMP) regulates extracellular type I collagen degradation by chondrocytes and endothelial cells. J. Cell Sci. 87:357-362.
10. Harlow, E. and D. Lane. 1994. Immunizations, p. 110. In Anonymous (Ed.), Antibodies A Laboratory Manual, 1st ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
11. Herron, G.S., M.J. Banda, E.J. Clark, J. Gavrilovic and Z. Werb. 1986. Secretion of metalloproteinases by stimulated capillary endothelial cells. J. Biol. Chem. 267:2814-2818.
12. Hodges, D.J., D.C. Lee, C.J. Salter, D.G. Reid, G.P. Harper and T.E. Cawston. 1994. Purification and secondary structural analysis of tissue inhibitor of metalloproteinases-1. Biochim. Biophys. Acta Protein Struct. Mol. Enzymol. 1208:94-100.
13. Kimball, J.W. 1986. Introduction to Immunology. Mcmillan, London.
14. Kleinman, H.K., M.L. McGarvey, J.R. Hassell, V.L. Star, F.B. Cannon, G.W. Laurie and G.R. Martin 1986. Basement membrane complexes with biological activity. Biochemistry 25:312-318.
15. Lynch, C.M., M.M. Clowes, W.R.A. Osborne, A.W. Clowes and A.D. Miller 1992. Long-term expression of human adenosine deaminase in vascular smooth muscle cells of rats: a model for gene therapy. Proc. Natl. Acad. Sci. USA 89:1138-1142.
16. Maniatis, T., E.F. Fritsch and J. Sambrook. 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. p. 109-112.
17. Matrisian, L.M. 1990. Metalloproteinases and their inhibitors in matrix remodeling. Trends Genet. 6:121-125.
18. Stricklin, G.P. 1986. Human fibroblast tissue inhibitor of metalloproteinases: glycosylation and function. Coll. Relat. Res. 6:219-228.
19. Stricklin, G.P. and H.G. Welgus. 1983 Human skin fibroblast collagenase inhibitor. J. Biol. Chem. 255:12252-12258.
20. Tang, D., M. DeVit and S.A. Johnston. 1992. Genetic immunization is a simple method for eliciting an immune response. Nature 356:152-154.
21. Warner, J.F., C. Anderson, L. Laube, D. J. Jolly, D. J. Jolly, K. Townsend, S. Chada and D. St. Louis. 1991. Induction of HIV-specific CTL and antibody responses in mice using retroviral vector-transduced cells. AIDS Res. Hum. Retroviruses 7:645-655.
22. Welgus, H.G. and G.P. Stricklin. 1983. Human skin fibroblast collagenase inhibitor: comparative studies in human connective tissues, serum, and amniotic fluid. J. Biol. Chem. 255:12259-12264.
23. Wurm, F.M., K.A. Gwinn and R. Kingston. 1986. Inducible overproduction of the mouse c-myc protein in mammalian cells. Proc. Natl. Acad. Sci. USA 83:5414-5418.