Quantification of polymerase chain reaction products: Enzyme immunoassay based systems for digoxigenin- and biotin-labelled products that quantify either total or specific amplicons

Molecular and Cellular Probes

Volumn 10, Issue 1, 1996, Pages 31-41

  Stevens, J ^a   Yu, F S ^a   Hassoun, P M ^a   Lanzillo, J J ^a  

^a TUFTS MEDICAL CENTER   (United States)

Author keywords

Biotin; Digoxigenin; Enzyme immunoassay; Polymerase chain reaction

Indexed keywords

ALKALINE PHOSPHATASE; ANTIBODY CONJUGATE; BIOTIN; DIGOXIGENIN; DNA; STREPTAVIDIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CHEMOLUMINESCENCE; DNA DETERMINATION; DNA PROBE; ENZYME IMMUNOASSAY; METHODOLOGY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ANIMALIA;

EID: 0029875477     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.1996.0005     Document Type: Article

References (52)

1. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B. & Erlich, H. A. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487-91.
2. Mullis, K. B. & Faloona, F. A. (1987). Specific synthesis of DNA in vitro via a polymerase catalyzed chain reaction. Methods Enzymology 155, 335-50.
3. Mullis, K. B., Faloona, F. A., Scharf, S. J., Horn, G. T. & Erlich, H. A. (1986). Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symposium on Quantitative Biology 51, 263-73.
4. Saiki, R. K., Walsh, P. S., Levenson, C. H. & & Erlich, H. A. (1989). Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes. Proceedings of the National Academy of Science of the USA 86, 6230-4.
5. Powell, E. E. & Kroon, P. A. (1992). Measurement of messenger RNA by quantitative PCR with a non-radioactive label. Journal of Lipid Research 33, 609-14.
6. Takeda, S., Shimazoe, T., Sato, K., Sugimoto, Y., Tsuruo, T. & Kono, A. (1992). Differential expression of DNA topoisomerase-I gene between CPT-11 acquired-resistant and native-resistant human pancreatic tumor cell lines detected by RNA/PCR based quantitation assay. Biochemistry Biophysics Research Communications 184, 618-25.
7. Delidow, B. C., Peluso, J. J. & White, B. A. (1989). Quantitative measurement of mRNAs by polymerase chain reaction. Genetics Analysis Techniques 6, 120-4.
8. Katz, J. P., Bodin, E. T. & Coen, D. M. (1990). Quantitative polymerase chain reaction analysis of herpes simplex virus DNA in ganglia of mice infected with replication-incompetent mutants. Journal of Virology 64, 4288-4295.
9. Wang, A. M., Doyle, M. V. & Mark, D. F. (1989). Quantification of mRNA by the polymerase chain reaction. Proceedings of the National Academy of Sciences USA 86, 9717-21.
10. Gilliland, G., Perrin, S., Blanchard, K. & Bunn, H. F. (1990). Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proceedings of the National Academy of Sciences USA 87, 2725-9.
11. Murphy, L. D., Herzog, C. E., Rudick, J. B., Fojo, A. T. & Bates, S. E. (1990). Use of the polymerase chain reaction in the quantitation of mdr-1 gene expression. Biochemistry 29, 10351-6.
12. Landgraf, A., Reckmann, B. & Rngoud, A. (1991). Quantitative analysis of polymerase chain reaction (PCR) products using primers label led with biotin and a fluorescent dye. Analytical Biochemistry 193, 231-5.
13. Terouanne, B., Balaguer, P., Boussioux, A. M. & Nicolas, J. C. (1992). Quantitative and qualitative analysis of amplified DNA sequences by a competitive hybridization assay. Analytical Biochemistry 205, 193-9.
14. Vlieger, A. M., Medenblik, A. M. J. C., Vangijlswijk, R. P. M., Tanke, H. J., Vanderploeg, M., Gratama, J. W. & Raap, A. K. (1992). Quantitation of polymerase chain reaction products by hybridization-based assays with fluorescent, colorimetric, or chemiluminescent detection. Analytical Biochemistry 205, 1-7.
15. Inouye, S. & Hondo, R. (1990). Microplate hybridization of amplified viral DNA segment. Journal of Clinical Microbiology 28, 1469-72.
16. Keller, G. H., Huang, D.-P., Shih, W.-K., & Manak, M. M. (1990). Detection of hepatitis B virus DNA in serum by polymerase chain reaction amplification and microtiter sandwich hybridization. Journal of Clinical Microbiology 28, 1411-16.
17. Nagata, Y., Yokota, H., Kosuda, O., Yokoo, K., Takemura, K. & Kikuchi, T. (1985). Quantification of picogram levels of specific DNA immobilized in microtiter wells. FEBS Letters 183, 379-82.
18. Balaguer, P., Terouanne, B., Boussioux, A. M. & Nicolas, J. C. (1991). Quantification of DNA sequences obtained by polymerase chain reaction using a bioluminescent adsorbent. Analytical Biochemistry 195, 105-10.
19. Landgraf, A., Reckmann, B. & Pingoud, A. (1991). Direct analysis of polymerase chain reaction products using enzyme-linked immunosorbent assay techniques. Analytical Biochemistry 198, 86-91.
20. Mantero, G., Zonaro, A., Albertini, A., Bertolo, P. & Primi, D. (1991). DNA enzyme immunoassay: general method for detecting products of polymerase chain reaction. Clinical Chemistry 37, 422-9.
21. Lundeberg, J., Wahlberg, J., Holmberg, M., Pettersson, U. & Uhlen, M. (1990). Rapid colorimetric detection of in vitro amplified DNA sequences. DNA Cell Biology 9, 287-92.
22. Syvanen, A., Bengtstrom, M., Tenhunen, J. & Soderlund, H. (1988). Quantification of polymerase chain reaction products by affinity-based hybrid collection. Nucleic Acids Research 16, 11327-38.
23. Sauvaigo, S., Fouque, B., Roget, A., Livache, T., Bazin, H., Chypre, C. & Teoule, R. (1990). Fast solid support detection of PCR amplified viral DNA sequences using radioiodinated or hapten labelled primers. Nucleic Acids Research 18, 3175-83.
24. Lanzillo, J. J. (1991). Chemiluminescent nucleic acid detection with digoxigenin-labelled probes: a model system with probes for angiotensin converting enzyme which detect less than one attomole of target DNA. Analytical Biochemistry 194, 45-53.
25. Rasmussen, S. R., Larsen, M. R. & Rasmussen, S. E. (1991). Covalent immobilization of DNA onto polystyrene microwells: the molecules are only bound at the 5′-end. Analytical Biochemistry 198, 138-42.
26. Johannsson, A., Stanley, C. J. & Self, C. H. (1985). A fast highly sensitive colorimetric enzyme immunoassay system demonstrating benefits of enzyme amplification in clinical chemistry. Clinical Chimica Acta 148, 119-24.
27. Lacy, M. J. & Voss, E. W. (1989). Direct adsorption of ssDNA to polystyrene for characterization of the DNA/ anti-DNA interaction, and immunoassay for anti-DNA autoantibody in New Zealand white mice. Journal of Immunological Methods 116, 87-98.
28. Lanzillo, J. J., Kong, X. J. & Fanburg, B. L. (1994). A competitive deletion mutant quantitative PCR assay for angiotensin-coverting enzyme mRNA in smooth muscle cells. PCR Methods and Applications 4, 167-71.
29. Suzuki, K., Okamoto, N., Watanabe, S. & Kano, T. (1992). Chemi luminescent microtiter method for detecting PCR amplified HIV-1 DNA. Journal of Virological Methods 38, 113-22.
30. Holmstrom, K., Rossen, L. & asmussen, O. F. (1993). A highly sensitive and fast nonradioactive method for detection of polymerase chain reaction products. Analytical Biochemistry 209, 278-83.
31. Kemp, D. J. (1992). Colorimetric detection of polymerase chain reaction-amplified DNA segments using DNA-binding proteins. Methods in Enzymology 216, 116-26.
32. Laskey, R. A. (1980). The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis. Methods in Enzymology 65, 363-71.
33. Chevrier, D., Rasmussen, S. R. & Guesdon, J. L. (1993). PCR product quantification by non-radioactive hybridization procedures using an oligonucleotide covalently bound to microwells. Molecular and Cellular Probes 7, 187-97.
34. Keller, G. H., Huang, D. P. & Manak, M. M. (1989). A sensitive nonisotopic hybridization assay for HIV-1 DNA. Analytical Biochemistry 177, 27-32.
35. Kohsaka, H., Taniguchi, A., Richman, D. D. & Carson, D. A. (1993). Microtiter format gene quantification by covalent capture of competitive PCR products: application to HIV-1 detection. Nucleic Acids Research 21, 3469-72.
36. Tada, J., Ohashi, T., Nishimura, N., Ozaki, H., Fukushima, S., Takano, J., Nishibuchi, M. & Takeda, Y. (1992). Non-isotopic microtitre plate-based assay for detecting products of polymerase chain reaction amplification: application to detection of the tdh gene of vibrio-parahaemolyticus. Molecular and Cellular Probes 6, 489-94.
37. Lopez, E., Chypre, C., Alpha, B. & Mathis, G. (1993). Europium(III) trisbipyridine cryptate label for time-resolved fluorescence detection of polymerase chain reaction products fixed on a solid support. Clinical Chemistry 39, 196-201.
38. Conway, B., Bechtel, L. J., Adler, K. A., Daquila, R. T., Kaplan, J. C. & Hirsch, M. S. (1992). Comparison of spot-blot and microtitre plate methods for the detection of HIV-1 PCR products. Molecular and Cellular Probes 6, 245-9.
39. Jalava, T., Lehtovaara, R., Kallio, A., Ranki, M. & Soderlund, H. (1993). Quantification of hepatitis-b virus DNA by competitive amplification and hybridization on microplates. BioTechniques 15, 134.
40. Holloway, B., Erdman, D. D., Durigon, E. L. & Murtagh, J. J. (1993). An exonuclease-amplification coupled capture technique improves detection of PCR product. Nucleic Acids Research 21, 3905-6.
41. King, J. A. & Ball, J. K. (1993). Detection of HIV-1 by digoxigenin-labelled PCR and microtitre plate solution hybridisation assay and prevention of PCR carry-over by uracil-n-glycosylase. Journal of Virological Methods 44, 67-76.
42. Sabbatini, A. R. M., Werner, P. A., Guha, C., Paddock, G. V. & Galbraith, R. M. (1993). The vitamin D-binding protein gene-quantitation of amplified nucleic acids by ELISA. BioTechniques-15, 706-13.
43. Alard, P., Lantz, O., Sebagh, M., Calvo, C. F., Weill, D., Chavanel, G., Senik, A. & Charpentier, B. (1993). A versatile ELISA-PCR assay for messenger RNA quantitation from a few cells. BioTechniques 15, 730-7.
44. Yang, B. Z., Yolken, R. & Viscidi, R. (1993). Quantitative polymerase chain reaction by monitoring enzymatic activity of DNA polymerase. Analytical Biochemistry 208, 110-16.
45. Galindo, I., Rangelaldao, R. & Ramirez, J. L. (1993). A combined polymerase chain reaction-colour development hybridization assay in a microtitre format for the detection of clostridium spp. Applied Microbiology and Biotechnology 39, 553-7.
46. Kawai, S., Maekawajiri, S. & Yamane, A. (1993). A simple method of detecting amplified DNA with immobilized probes on microtiter wells. Analytical Biochemistry 209, 63-9.
47. Magalini, A. R., Primi, D., Albertini, A., Capucci, A., Rossi, G. & Mantero, G. (1993). Detection of BCR/ ABL transcripts in chronic myeloid leukaemia by polymerase chain reaction and DNA enzyme immunoassay: a DNA probe assay without DNA labelling. British Journal of Haematology 83, 334-9.
48. Rapier, J. M., Villamarzo, Y., Schochetman, G., Ou, C. Y., Brakel, C. L., Donegan, J., Maltzman, W., Lee, S., Kirtikar, D. & Gatica, D. (1993). Nonradioactive, colorimetric microplate hybridization assay for detecting amplified human immunodeficiency virus DNA. Clinical Chemistry 39, 244-7.
49. Soubrier, F., Alhenc-Gelas, F., Hubert, C., Allegrini, J., John, M., Tregear, G. & Corvol, P. (1988). Two putative active centers in human angiotensin 1-converting enzyme revealed by molecular cloning. Proceedings of the National Academy of Sciences USA 85, 9386-90.
50. Shai, S. Y., Fishel, R. S., Martin, B. M., Berk, B. C. & Bernstein, K. E. (1992). Bovine angiotensin converting enzyme cDNA cloning and regulation: increased expression during endothelial cell growth arrest. Circulation Research 70, 1274-81.
51. Arcari, P., Martinelli, R. & Salvatore, F. (1984). The complete sequence of a full length cDNA for human liver glyceraldehyde-3-phosphate dehydrogenase: evidence for multiple mRNA species. Nucleic Acids Research 12, 9179-89.
52. Amaya, Y., Yamazaki, K., Sato, M., Noda, K., Nishino, T. & Nishino, T. (1990). Proteolytic conversion of xanthine dehydrogenase from the NAD-dependent type to the oxygen-dependent type. Journal of Biological Chemistry 265, 14170-5.