Purification and refolding of recombinant human proMMP-7 (pro-matrilysin) expressed in Escherichia coli and its characterization

Journal of Biochemistry

Volumn 119, Issue 4, 1996, Pages 667-673

  Itoh, Michiyasu ^a   Masuda, Kazuto ^a   Ito, Yuko ^a   Akizawa, Toshifumi ^a   Yoshioka, Masanori ^a   Imai, Kazushi ^b   Okada, Yasunori ^b   Sato, Hiroshi ^b   Seiki, Motoharu ^b  

^a SETSUNAN UNIVERSITY   (Japan)

^b KANAZAWA UNIVERSITY   (Japan)

Author keywords

MMP inhibitors; Ni NTA resin; Recombinant matrylisin; Recombinant MMP 7; Zymogen activation

Indexed keywords

CALCIUM ION; ENZYME INHIBITOR; ENZYME PRECURSOR; MATRILYSIN; PHENYLMERCURIC ACETATE; RECOMBINANT PROTEIN; SYNTHETIC PEPTIDE; TISSUE INHIBITOR OF METALLOPROTEINASE; TRANSFERRIN; UREA; ZINC;

ARTICLE; AUTOREGULATION; CARBOXY TERMINAL SEQUENCE; CELL LYSATE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; HUMAN; NONHUMAN; PEPTIDE MAPPING; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FOLDING;

EID: 0029875424     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021294     Document Type: Article

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