Enzyme-linked immunosorbent assay and immunoblot analysis for detection of antibodies to Borrelia burgdorferi in dogs: The impact of serum absorption with homologous and heterologous bacteriae

Veterinary Microbiology

Volumn 48, Issue 3-4, 1996, Pages 257-268

  Wittenbrink, Max M ^a   Failing, Klaus ^a   Krauss, Hartmut ^a  

^a NONE

Author keywords

Borrelia burgdorferi; Dog; ELISA; Immunoblot; Serum absorption

Indexed keywords

ABSORPTION; ANIMAL EXPERIMENT; ANTIBODY DETECTION; ARTICLE; BORRELIA BURGDORFERI; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DOG; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOBLOTTING; LYME DISEASE; NONHUMAN; SERODIAGNOSIS; SERUM;

ANIMALS; ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; BLOTTING, WESTERN; BORRELIA BURGDORFERI GROUP; DOG DISEASES; DOGS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; IXODES; LYME DISEASE;

EID: 0029874182     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1135(95)00165-4     Document Type: Article

References (35)

1. Appel, M.J.G., Allan, S., Jacobson, R.H., Lauderdale, T.L., Chang, Y.F., Shin, S.J., Thomford, J.W., Todhunter, R.J. and Summers, B.A. 1993. Experimental Lyme disease in dogs produces arthritis and persistent infection. J. Infect. Dis., 167: 651-664.
2. Barbour, A.G., 1984. Isolation and cultivation of Lyme disease spirochetes. Yale J. Biol. Med., 57: 521-525.
3. Breitschwerdt, E.B., Nicholson, W.L., Kiehl, A.R., Steers, C., Meuten, D.J. and Levine, J.F., 1994. Natural infections with Borrelia spirochetes in two dogs from Florida. J. Clin. Microbiol., 32: 352-357.
4. Cooke, W.D., Orr, A.S., Wiseman, B.L., Rouse, S.B., Murray, W.C. and Ranck, S.G., 1993. Human cord blood contains an IgM antibody to the 41 kD flagellar antigen of Borrelia burgdorferi. Scand. J. Immunol., 38: 407-409.
5. Cooke, W.D. and Bartenhagen, N.H., 1994. Seroreactivity to Borrelia burgdorferi antigens in the absence of Lyme disease. J. Rheumatol., 21: 126-131.
6. Cutler, S.J. and Wright, D.J.M., 1994. Predictive value of serology in diagnosing Lyme borreliosis. J. Clin. Pathol., 47: 344-349.
7. Dixon, W.J., 1993. BMDP Statistical Software Manual, Vol.1 and 2. University of California Press, Berkeley, Los Angeles, London.
8. Eisensmith, S.P., 1993. PlotIT - Graphics qne Statistics. Scientific Programming Enterprises.
9. Eng, T.R., Wilson, M.L., Spielman A. and Lastavica, C.C., 1988. Greater risk of Borrelia burgdorferi infection in dogs than in people. J. Infect. Dis., 158: 1410-1411.
10. Fawcett, P.T., O'Brien, A.E. and Doughty, R.A., 1989. An absorption procedure to increase the specificity of enzyme-linked immunosorbent assays for Lyme disease without decreasing sensitivity. Arthr. Rheumat., 32: 1041-1044.
11. Gitton, X., Buggin Daubie, M., Anfre, F., Ganiere, J.P. and Andre-Fontaine, G., 1994. Recognition of Leptospira interrogans antigens by vaccinated or infected dogs. Vet. Microbiol., 41: 87-97.
12. Greene, R.T., 1991. Canine Lyme Borreliosis. Small Anim. Pract., 21: 51-64.
13. Greene, R.T., Walker, R.L., Nicholson, W.L., Heidner, H.W., Levine, J.F., Burgess, E.C., Wyand, M., Breitschwerdt, E.B. and Berkhoff, H.A., 1988. Immunoblot analysis of immunoglobulin G response to the Lyme disease agent (Borrelia burgdorferi) in experimentally and naturally exposed dogs. J. Clin. Microbiol., 26: 648-653.
14. Greene, R.T., Hirsch, D.A., Rottmann, P.L. and Gerig, T.M., 1991. Interlaboratory comparison of liters of antibody to Borrelia burgdorferi and evaluation of commercial assay using canine sera. J. Clin. Microbiol., 29: 16-20.
15. Hellmann, E. and Aschenbrenner, E., 1987. Untersuchungen über die Eignung des ELISA für den Nachweis von Antikörpern gegen Leptospiren beim Hund. Tierärztl. Umschau, 42: 634-638.
16. Kaslow, R.A., 1992. Current perspective on Lyme Borreliosis. J. Amer. Med. Assoc., 267: 1381-1383.
17. Käsbohrer, A., Liebisch, G., Schönberg, A. and Liebisch, A., 1994. Serodiagnostik der Lyme-Borreliose: Entwicklung und Bewertung eines Testsystems für epidemiologische Untersuchungen beim Hund. Dtsch. Tierärztl. Wschr., 101: 476-481.
18. Kowal, K. and Weinstein, A., 1994. Western blot band intensity analysis - Application to the diagnosis of Lyme arthritis. Arthr.Rheumat., 37: 1206-1211.
19. Komblatt, A.N., Urband, P.H. and Steere, A.C., 1985. Arthritis caused by Borrelia burgdorferi in dogs. J. Am. Vet. Med. Assoc., 186: 960-964.
20. Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
21. Magnarelli, L.A., Anderson, J.F., Schreier, A.B. and Ficke, C.M., 1987. Clinical and serologic studies of canine borreliosis. J. Am. Vet. Med. Assoc., 191: 1089-1094.
22. Meier, C., Srisopar, B. and Amtsberg, G., 1982. Untersuchungen zum Vorkommen von Treponemen bei Hunden mit Darmerkrankungen. Berl. Münch. Tierärztl. Wschr., 95: 188-193.
23. Pindak, F.F., Clapper, W.E. and Sherrod, J.H., 1965. Incidence and distribution of spirochetes in the digestive tract of dogs. Amer. J. Vet. Res., 26: 1391-1402.
24. Raoult, D., Hechemy, K.E. and Baranton, G., 1989. Cross-reaction with Borrelia burgdorferi antigen from patients with Human Immunodeficiency Virus infection, Syphilis, and Leptospirosis. J. Clin. Microbiol., 27: 2152-2155.
25. Reed, S.M. and Northcote, D.H., 1981. Minimization of variation in the response to different proteins of the Coomassie Blue G dye-binding assay for protein. Analyt. Biochem., 116: 53-63.
26. Sadziene, A., Thompson, P.A. and Barbour, A.G., 1993. In vitro inhibition of Borrelia burgdorferi growth by antibodies. J. Infect. Dis., 167: 165-172.
27. Sayahtaheri-Altaie, S., Meier, F.A. and Dalton, H.P., 1993. Identification of species-specific, non-cross-reactive proteins of Borrelia burgdorferi. Diagn. Microbiol. Infect. Dis., 16: 43-51.
28. Schillhorn van Veen, T.W., Murphy, A.J. and Colmery B., 1993. False positive Borrelia burgdorferi antibody titres associated with periodontal disease in dogs. Vet. Rec., 132: 512.
29. Schönberg, A., Staak, C. and Kämpe, U., 1987. Leptospirose in der Bundesrepublik Deutschland. J. Vet. Med. B, 34: 98-108.
30. Shin, S.J., Chang, Y.F., Jacobson, R.H., Shaw, E., Lauderdale, T.L., Appel, M.J. and Lein, D.H., 1993. Cross-reactivity between B. burgdorferi and other spirochetes affects specificity of serotests for detection of antibodies to the Lyme disease agent in dogs. Vet. Microbiol., 36: 161-174.
31. Shoberg, R.S., Jonsson, M., Sadziene, A., Bergström, S. and Thomas, D.D., 1994. Identification of a highly cross-reactive outer surface protein B epitope among diverse geographic isolates of Borrelia spp. causing Lyme disease. J. Clin. Microbiol., 32: 489-500.
32. Sigal, L.H., 1993. Cross-reactivity between Borrelia burgdorferi flagellin and a human axonal 64.000 molecular weight protein. J. Infect. Dis., 167: 1372-1378.
33. Towbin, H., Staehelin, T. and Gordon, J., 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA, 76: 4350-4354.
34. Wallich, R., Moter, S.E., Simon, M.M., Ebnet, K., Heiberger, A. and Kramer, M.D., 1990. The Borrelia burgdorferi flagellum-associated 41-kilodalton antigen (flagellin): molecular cloning, expression, and amplification of the gene. Infect. Immun., 58: 1711-1719.
35. Wilske, B., Preac-Mursic, V., Göbel, U., Graf, B., Jauris, S., Soutschek, E., Schwab, E. and Zumstein, G., 1993. An OspA serotyping system for Borrelia burgdorferi based on reactivity with monoclonal antibodies and OspA sequence analysis. J. Clin. Microbiol., 31: 340-350.