Stable functional expression of the adult subtype of human muscle acetylcholine receptor following transfection of the human rhabdomyosarcoma cell line TE671 with cDNA encoding the ε subunit

Neuroscience Letters

Volumn 207, Issue 1, 1996, Pages 57-60

  Beeson, D ^a   Amar, M ^b   Bermudez, I ^b   Vincent, A ^a   Newsom Davis, J ^a  

^a JOHN RADCLIFFE HOSPITAL   (United Kingdom)

^b OXFORD BROOKES UNIVERSITY   (United Kingdom)

Author keywords

Acetylcholine receptor; Myasthenia gravis; TE671 cells; Transfection; subunit

Indexed keywords

CHOLINERGIC RECEPTOR; COMPLEMENTARY DNA; RECEPTOR SUBUNIT;

ADULT; ARTICLE; CELL CULTURE; CELL LINE; CONTROLLED STUDY; ELECTROPHYSIOLOGY; FETUS; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN TISSUE; MYASTHENIA GRAVIS; PRIORITY JOURNAL; RECEPTOR BINDING; RHABDOMYOSARCOMA;

EID: 0029874077     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/0304-3940(96)12488-5     Document Type: Article

References (17)

1. Beeson, D., Brydson, M., Betty, M., Jeremiah, S., Povey, S., Vincent, A. and Newsom-Davis, J., Primary structure of the human muscle acetylcholine receptor: cDNA cloning of the γ and ε. subunits, Eur. J. Biochem., 215 (1993) 229-238.
2. Claudio, T., Molecular genetics of acetylcholine receptor channels. In D.M. Glover and B.D. Hames (Eds.), Molecular Neurobiology, IRL Press, Oxford, 1989, pp. 63-142.
3. Conroy, W., Saedi, M. and Lindstrom, J., TE671 cells express an abundance of partially mature acetylcholine receptor α subunit which has characteristics of an assembly intermediate, J. Biol. Chem., 265 (1990) 21642-21651.
4. Fambrough, D.M., Control of acetylcholine receptors in skeletal muscle, Physiol. Rev., 59 (1979) 165-227.
5. Gu, Y., Franco, A., Gardner, P., Lansman, J., Forsayeth, J. and Hall, Z., Properties of embryonic and adult muscle acetylcholine receptors transiently expressed in COS cells. Neuron, 5 (1990) 147-157.
6. Luther, M., Schoepfer, R., Whiting, P., Casey, B., Blatt, Y., Montai, M.S., Montal, M. and Lindstrom, J., A muscle acetylcholine receptor is expressed in the human cerebellar medulloblastoma cell line TE671, J. Neurosci., 9 (1989) 1082-1096.
7. McAllister, R., Isaacs, H., Rongey, R., Peer, M., Au, W., Sonkup, S. and Gardner, M., Establishment of a human medulloblastoma cell line, Int. J. Cancer, 20 (1977) 206-212.
8. Milone, M., Hutchinson, D. and Engel, A., Patch-clamp analysis of the properties of acetycholine receptor channels at the normal human endplate, Muscle Nerve, 17 (1994) 1364-1369.
9. Mishina, N., Takai, T., Imoto, K., Noda, M., Takahashi, T., Numa, S., Methfessel, C. and Sakmann, B., Molecular distinction between fetal and adult forms of muscle acetylcholine receptor, Nature, 321 (1986) 406-411.
10. Morris, A., Beeson, D., Jacobson, L., Baggi, F., Vincent, A. and Newsom-Davis, J., Two isoforms of muscle acetylcholine receptor a subunit are translated in the human cell line TE671, FEBS Lett., 295 (1991) 116-118.
11. Newland, C., Beeson, D., Vincent, A. and Newsom-Davis, J., Functional and non-functional isoforms of the human muscle acetylcholine receptor, J. Physiol. (London), 489 (1995) 767-778.
12. Sine, S., Functional properties of human skeletal muscle acetylcholine receptors expressed by the TE671 cell line, J. Biol. Chem., 263 (1988) 18052-18062.
13. Somnier, F., Anti-acetylcholine receptor (AChR) antibodies measurement in myasthenia gravis: the use of cell line TE671 as a source of AChR antigen, J. Neuroimmunol., 51 (1994) 63-68
14. Vincent, A., Newland, C., Brueton, L., Beeson, D., Riemersma, S., Huson, S. and Newsom-Davis, J., Arthrogryposis multiplex congenita with maternal autoantibodies specific for a fetal antigen. Lancet, 364 (1995) 24-25.
15. Walker, R., Vincent, A. and Newsom-Davis, J., Immunological and pharmacological heterogeneity of α-bungarotoxin binding sites extracted from TE671 cells, J. Neuroimmunol., 19 (1988) 149-157.
16. Weinberg, C. and Hall, Z., Antibodies from patients with myasthenia gravis recognise determinants unique to extrajunctional acetylcholine receptors, Proc.Natl.Acad.Sci.USA,76 (1979) 504-508.
17. Whiting, P., Vincent, A., Schluep, M. and Newsom-Davis, J., Monoclonal antibodies that distinguish between normal and denervated human muscle acetylcholine receptor, J. Neuroimmunol., 11 (1986) 223-235.