Comparison of protocols for depleting anti-E. coli antibody in immunoblotting of recombinant antigens

Journal of Immunological Methods

Volumn 190, Issue 1, 1996, Pages 143-145

  Covini, Giovanni ^a,b   Chan, Edward K L ^a   Colombo, Massimo ^b   Tan, E M ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b Istituto di Medicina Interna   (Italy)

Author keywords

Anti E. call antibody depletion; Autoantibody; Immunoblotting

Indexed keywords

AUTOANTIBODY; RECOMBINANT PROTEIN;

ANTIBODY DETECTION; ARTICLE; ESCHERICHIA COLI; HUMAN; IMMUNOBLOTTING; PRIORITY JOURNAL;

ESCHERICHIA COLI;

EID: 0029873684     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(95)00288-X     Document Type: Article

References (4)

1. Chan, E.K.L. and Pollard, K.M. (1992) Autoantibodies to ribonucleoprotein particles by immunoblotting. In: N.R. Rose, E.C. de Macario, J.L. Fahey, H. Friedman and G.M. Penn (Eds.), Manual of Clinical Laboratory Immunology, Vol. 4. American Society for Microbiology, Washington, DC, p. 755.
2. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680.
3. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Plasmid vectors. In: N. Ford, C. Nolan and M. Ferguson (Eds.), Molecular Cloning, Vol. II, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, p. 1.1.
4. Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350.