-
4
-
-
0026780670
-
-
B. A Morgan, J. C. Izpisua-Belmonte, D. Duboule, C. J. Tabin, Nature 358, 236 (1992)
-
(1992)
Nature
, vol.358
, pp. 236
-
-
Morgan, B.A.1
Izpisua-Belmonte, J.C.2
Duboule, D.3
Tabin, C.J.4
-
6
-
-
0028103855
-
-
W. J. Gehring et al , Cell 78, 211 (1994).
-
(1994)
Cell
, vol.78
, pp. 211
-
-
Gehring, W.J.1
-
12
-
-
15144360480
-
-
M. H. Polymeropoulos, R I. Ortez deLuna, S. E. Ide, C. A. Francomano, Am. J. Hum Gen. 57 (suppl.), A200 (1995).
-
(1995)
Am. J. Hum Gen.
, vol.57
, Issue.SUPPL.
-
-
Polymeropoulos, M.H.1
Ortez DeLuna, R.I.2
Ide, S.E.3
Francomano, C.A.4
-
14
-
-
0026427261
-
-
J C. Izpisúa-Belmonte, C Tickle, P. Dollé, L. Wolpert, D. Duboule, Nature 350, 585 (1991).
-
(1991)
Nature
, vol.350
, pp. 585
-
-
Izpisúa-Belmonte, J.C.1
Tickle, C.2
Dollé, P.3
Wolpert, L.4
Duboule, D.5
-
16
-
-
15844367767
-
-
note
-
For sequencing of exon 1 of the HOXD13 gene the cosmid clone G2 (15), containing the homeobox genes HOXD10, HOXD11, HOXD12, HOXD13, and EVX2, was digested with Eco RI, and a 10-kb fragment containing the entire HOXD13 and the 5′ half of the HOXD12 gene was subcloned in the Eco RI site of pBluescript With this fragment as a template, the sense primer 5′-TTTGTATCAGGGQATGTGGC-3′ and the antisense primer 5′-CCACATCAGGAGA-CAGTATC-3′ were used to amplify exon 2 of the HOXD13 gene by PCR. A 1.3-kb Pst I fragment derived from the 10-kb Eco RI fragment hybridized with the PCR product and was therefore subcloned in the Pst I site of pBluescript and sequenced by dideoxynucleotide cycle sequencing. Because the 5′ end of the sequence of the Pst I fragment did not reach the translational start codon ATG of the HOXD13 gene, the primer 5′-ACATACGGCAGCTG-TAGTAG-3′, corresponding to a sequence 70 bp downstream of the 5′ Pst I site, was used for sequencing with the 10-kb Eco RI fragment as the template
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15844368752
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note
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2, 200 μM deoxynucleotide triphosphate (dNTP) without dGTP, 200 μM 7-deaza-2′-deoxyguanosine 5′-triphosphate, a final concentration of 0 5 μM for each primer, 10% dimethyl sulfoxide, and 0.2 U of Taq polymerase. The PCR products were analyzed on a 5% polyacrylamide gel under nondenaturing conditions. PCR products from affected individuals were subcloned into pCRII (invitrogen) and subjected to cycle sequencing
-
-
-
-
20
-
-
0025192434
-
-
J D. Licht, M. J Grossel, J. Figge, U M Hansen, ibid. 346, 76 (1990).
-
(1990)
Nature
, vol.346
, pp. 76
-
-
Licht, J.D.1
Grossel, M.J.2
Figge, J.3
Hansen, U.M.4
-
22
-
-
0027169185
-
-
_, EMBO J 12, 2723 (1993).
-
(1993)
EMBO J
, vol.12
, pp. 2723
-
-
-
23
-
-
0027358721
-
-
P. Dolle et a l, Cell 75, 431 (1993).
-
(1993)
Cell
, vol.75
, pp. 431
-
-
Dolle, P.1
-
25
-
-
0026326323
-
-
P. Dolle, J. C Izpisua-Belmonte, E. Boncinelli, D Duboule, Mech. Dev 36, 3 (1991).
-
(1991)
Mech. Dev
, vol.36
, pp. 3
-
-
Dolle, P.1
Izpisua-Belmonte, J.C.2
Boncinelli, E.3
Duboule, D.4
-
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-
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15844391002
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note
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The cosmid clone G2, containing the HOXD13 gene, was provided by E Boncinelli We want to thank C Tabin for helpful discussions. Y. Pittel provided secretarial assistance Supported by NIH grants AR36819 and AR36820 (to B R.O.) and Deutsche Forschungsgemeinschaft (to S M.)
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