Pseudomonas keratitis: The role of an uncharacterized exoprotein, protease IV, in corneal virulence

Investigative Ophthalmology and Visual Science

Volumn 37, Issue 4, 1996, Pages 534-543

  O'Callaghan, Richard J ^a   Engel, Lee S ^a   Hobden, Jeffery A ^a   Callegan, Michelle C ^a   Green, Linda C ^a   Hill, James M ^a  

^a LOUISIANA STATE UNIV MEDICAL CENTER   (United States)

Author keywords

animal model; keratitis; protease; Pseudomonas; virulence

Indexed keywords

BACTERIAL ENZYME; PROTEINASE;

ANIMAL MODEL; ARTICLE; BACTERIAL VIRULENCE; CONTROLLED STUDY; ENZYME ASSAY; KERATITIS; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; RABBIT;

ADP RIBOSE TRANSFERASES; ANIMALS; BACTERIAL TOXINS; COLONY COUNT, MICROBIAL; CORNEA; EXOTOXINS; EYE INFECTIONS, BACTERIAL; FEMALE; KERATITIS; MICE; PEPTIDE HYDROLASES; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; RABBITS; SERINE ENDOPEPTIDASES; VIRULENCE; VIRULENCE FACTORS;

EID: 0029871886     PISSN: 01460404     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (58)

1. Laibson PR. Pseudomonas aeruginosa. In: Fraunfelder FT, Roy FH, eds. Current Ocular Therapy. Philadelphia: WB Saunders; 1990:35-37.
2. Steuhl K, Doring G, Henni A, Thiel H, Botzenhart K. Relevance of host-derived and bacterial factors in Pseudomonas aeruginosa corneal infections. Invest Ophthalmol Vis Sci. 1987;28:1559-1568.
3. Chusid MJ, Davis SD. Experimental bacterial keratitis in neutropenic guinea pigs: Polymorphonuclear leukocytes in corneal host defense. Infect Immun. 1979;24:948-952.
4. Hobden JA, Engel LS, Callegan MC, Hill JM, Gebhardt BM, O'Callaghan RJ. Pseudomonas aeruginosa keratitis in leukopenic rabbits. Curr Eye Res. 1993;12:461-467.
5. Hobden JA, O'Callaghan RJ, Hill JM, Hagenah M, Insler MS, Reidy JJ. Ciprofloxacin and prednisolone therapy for experimental Pseudomonas keratitis. Curr Eye Res. 1992;11:259-266.
6. Engel LS, Callegan MC, Hobden JA, Hill JM, O'Callaghan RJ. Effectiveness of specific antibiotic/steroid combinations for the therapy of experimental Pseudomonas aeruginosa keratitis. Curr Eye Res. 1995;14:229-234.
7. Hazlett LD, Berk RS, Iglewski BH. Microscopic characterization of ocular damage produced by Pseudomonas aeruginosa exotoxin A. Infect Immun. 1981;34:1025-1035.
8. Hazlett LD, Iglewski BH, Berk RS. Experimental Pseudomonas exotoxin A mediated ocular damage in mouse pups: Microscopic observations. Ophthalmic Res. 1982;14:401-408.
9. Iglewski BH, Burns RP, Gipson IK. Pathogenesis of corneal damage from Pseudomonas exotoxin A. Invest Ophthalmol Vis Sci. 1977;16:73-76.
10. Ohman DE, Burns RP, Iglewski BH. Corneal infections in mice with toxin A and elastase mutant of Pseudomonas aeruginosa. J Infect Dis. 1980;142:547-555.
11. Vasil ML, Pritchard AE, Ostroff RM. Molecular biology of exotoxin A and phospholipase C of Pseudomonas aeruginosa. In: Silver S, Chakrabarty AM, Iglewski B, Kaplan S, eds. Pseudomonas: Biotransformations, Pathogenesis, and Evolving Biotechnology. Washington, DC: American Society for Microbiology; 1990:3-14.
12. Bejarano PA, Langeveld JPM, Hudson BG, Noelken ME. Degradation of basement membranes by Pseudomonas aeruginosa elastase. Infect Immun. 1989;57:3783-3787.
13. Heck LW, Morihara K, Abrahamson D. Degradation of soluble laminin and depletion of tissue-associated basement membrane laminin by Pseudomonas aeruginosa elastase and alkaline protease. Infect Immun. 1986;54:149-153.
14. Kawaharajo K, Homma JY. Pathogenesis of the mouse keratitis produced with Pseudomonas aeruginosa. Jpn J Exp Med. 1975;45:515-524.
15. Kessler E, Kennan HE, Brown SI. Pseudomonas protease, purification, partial characterization, and its effect on collagen, proteoglycan, and rabbit corneas. Invest Ophthalmol Vis Sci. 1977;16:488-497.
16. Kreger AS, Gray LD. Purification of Pseudomonas aeruginosa proteases and microscopic characterization of pseudomonal protease-induced rabbit corneal damage. Infect Immun. 1978;19:630-648.
17. Liu PV. The roles of various fractions of Pseudomonas aeruginosa in its pathogenesis: II: Effects of lecithinase and protease. J Infect Dis. 1966;116:112-116.
18. Nicas TI, Iglewski BH. The contribution of exoproducts to virulence of Pseudomonas aeruginosa. Can J Microbiol. 1985;31:387-392.
19. Wretlind B, Sjoberg L, Wadstrom T. Protease-deficient mutants of Pseudomonas aeruginosa: Pleiotropic changes in activity of other extracellular enzymes. J Gen Microbiol. 1977;103:329-336.
20. Hazlett LD, Moon MM, Singh A, Berk RS, Rudner H. Analysis of adhesion, piliation, protease production and ocular infectivity of several P. aeruginosa strains. Curr Eye Res. 1991;10:351-362.
21. Kernacki KA, Hobden JA, Hazlett LD, Fridman R, Berk RS. In vivo protease production during Pseudomonas aeruginosa corneal infection. Invest Ophthalmol Vis Sci. 1995;36:1371-1378.
22. Ohman DE, Sadoff JC, Iglewski BH. Toxin A-deficient mutants of Pseudomonas aeruginosa PA103: Isolation and characterization. Infect Immun. 1980;28:899-908.
23. Liu PV. Extracellular toxins of Pseudomonas aeruginosa. J Infect Dis. 1973;130:894-899.
24. Holder IA, Haidaris CG. Experimental studies of the pathogenesis of infections due to Pseudomonas aeruginosa: Extracellular protease and elastase as in vivo virulence factors. Can J Microbiol. 1979;25:593-599.
25. Tamura Y, Suzuk S, Sawada T. Role of elastase as a virulence factor in experimental Pseudomonas aeruginosa infection in mice. Microbiol Pathol. 1992;12:237-244.
26. Howe TR, Iglewski BH. Isolation and characterization of alkaline protease-deficient mutants of Pseudomonas aeruginosa in vitro and in a mouse eye model. Infect Immun. 1984;43:1058-1063.
27. Twining SS, Kirschner SA, Mahnke LA, Frank DW. Effect of Pseudomonas aeruginosa elastase, alkaline protease and exotoxin A on corneal proteases and proteins. Invest Ophthalmol Vis Sci. 1993;34:2699-2712.
28. Twining SS, Davis SD, Hyndiuk RA. Relationship between proteases and descemetocele formation in experimental Pseudomonas keratitis. Curr Eye Res. 1986;5:503-510.
29. Cryz SJ, Iglewski BH. Production of alkaline protease by Pseudomonas aeruginosa. J Clin Microbiol. 1980;12: 131-133.
30. Morihara K, Tsuzuki H. Production of protease and elastase by Pseudomonas aeruginosa strains isolated from patients. Infect Immun. 1977;15:679-685.
31. Guzzo J, Murgier M, Filloux A, Lazdunski A. Cloning of the Pseudomonas aeruginosa alkaline protease gene and secretion of the protease into the medium by Escherichia coli. J Bacteriol. 1989;172:942-948.
32. Jaffer-Bandjee MC, Carrere J, Bally M, Guy-Crotte O, Galbert C. Immunoenzymetric assays for alkaline protease and exotoxin A from Pseudomonas aeruginosa: Development and use in detecting exoproteins in clinical isolates from patients with cystic fibrosis. Eur J Clin Chem Clin Biochem. 1994;32:893-899.
33. Gambello MJ, Iglewski BH. Cloning and characterization of the Pseudomonas aeruginosa las R gene, a transcriptional activator of elastase expression. J Bacteriol. 1991;173:3000-3009.
34. Toder SD, Gambello MJ. Pseudomonas aeruginosa protease IV: Cloning of a caseinase which is not transcriptionally activated by the lasR. Presented at the 92nd General Meeting of the American Society for Microbiology; May 28, 1992.
35. Reller LB, Schoenknecht FD, Kenney MA, Sherris JC. Antibiotic susceptibility testing of Pseudomonas aeruginosa: Selection of a control strain and criteria for magnesium and calcium content in media. J Infect Dis. 1974;130:454-463.
36. Gritz DC, Lee TY, Kwitko S, McDonnell PJ. Topical anti-inflammatory agents in an animal model of keratitis. Arch Ophthalmol. 1990;108:1001-1005.
37. Hobden JA, O'Callaghan RJ, Hill JM, Reidy JJ, Rootman DS, Thompson, HW. Tobramycin iontophoresis into corneas infected with drug-resistant Pseudomonas aeruginosa. Curr Eye Res. 1989;8:1163-1169.
38. Hobden JA, O'Callaghan RJ, Insler MS, Hill JM. Ciprofloxacin ointment versus ciprofloxacin drops for the therapy of experimental Pseudomonas keratitis. Cornea. 1993;12:138-141.
39. Hobden JA, Reidy JJ, O'Callaghan RJ, Insler MS, Hill JM. Quinolones in collagen shields to treat aminoglycoside-resistant pseudomonal keratitis. Invest Ophthalmol Vis Sci. 1990;31:2241-2243.
40. Hobden JA, Rootman DS, O'Callaghan RJ, Hill JM. Iontophoretic application of tobramycin to uninfected and Pseudorrumas aeruginosa-infected rabbit corneas. Antimicrob Agents Chemother. 1988;32:978-981.
41. Reidy JJ, Hobden JA, Hill JM, Forman K, O'Callaghan RJ. The efficacy of topical ciprofloxacin and norfloxacin in the treatment of experimental Pseudomonas keratitis. Cornea. 1991;10:25-28.
42. Rootman DS, Hobden JA, Jantzen JA, Gonzalez JR, O'Callaghan RJ, Hill M. Iontophoresis of tobramycin for the treatment of experimental Pseudomonas keratitis in the rabbit. Arch Ophthalmol. 1988;106:262-265.
43. Chung DW, Collier RJ. Enzymatically active peptide from the adenosine diphosphate-ribosylating toxin of Pseudomonas aeruginosa. Infect Immun. 1977;16:832-841.
44. Bever R, Iglewski BH. Molecular characterization and molecule sequence of the Pseudomonas aeruginosa elastase structural gene. J Bacteriol. 1988;170:4309-4314.
45. Galloway DR, Hedstrom RC, Poulouskis OR. Production and characterization of monoclonal antibodies to exotoxin A from Pseudomonas aeruginosa. Infect Immun. 1984;44:262-267.
46. Herron GS, Banda MJ, Clark EJ, Gavrilovic J, Werb Z. Secretion of metalloproteinase by stimulated capillary endothelial cells: II: Expression of collagenase and stromolysin activities is regulated by endogenous inhibitors. J Biol Chem. 1986;261:2814-2818.
47. Heussen C, Dowdle EB. Electrophoretic analysis of plasminogen activators in polyacrylamide gel containing sodium dodecyl sulfate and copolymerized substrates. Anal Biochem. 1980;102:196-202.
48. Brockman EB, Tarantino PA, Hobden JA, et al. Keratotomy model of Pseudomonas aeruginosa keratitis: Gentamicin chemotherapy. Refract Corneal Surg. 1992;8: 39-43.
49. Johnson MK, Hobden JA, Hagenah M, O'Callaghan RJ, Hill JM, Chen S. The role of pneumolysin in ocular infections with Streptococcus pneumoniae. Curr Eye Res. 1990;9:1107-1114.
50. Gerke JR, Magliocco MV. Experimental Pseudomonas aeruginosa infections of the mouse cornea. Infect Immun. 1971;3:209-216.
51. SAS Institute, Inc. SAS Users Guide: Statistics, version 5. Gary NC, SAS Institute. 1985.
52. Hazlett LD, Masinick S, Barrett R, Rosol K. Evidence for asialo GM1 as a corneal glycolipid receptor for Pseudomonas aeruginosa adhesion. Infect Immun. 1993;61:5164-5173.
53. Miyagawa S, Kamata R, Matsumoto K, Okamura R, Maeda H. Therapeutic intervention with chicken egg white ovomacroglobulin and a new quinolone on experimental Pseudomonas keratitis. Graefes Arch Clin Exp Ophthalmol. 1994;232:488-493.
54. Kassler E, Spierer A. Inhibition by phosphoramidon of Pseudomonas aeruginosa elastase injected intracorneally in rabbit eyes. Curr Eye Res. 1984;3:1075-1078.
55. Burns FR, Paterson CA, Gray RD, Wells JT. Inhibition of Pseudomonas aeruginosa elastase and Pseudomonas keratitis using a thiol-based peptide. Antimicrob Agent Chemother. 1990;34:2065-2069.
56. Stuart JC, Turgeon PT, Kowalski RP. Aprotinin treatment of pseudomonal corneal infection. Cornea. 1991;10:63-66.
57. Levy JH, Katz HR. Effect of systemic tetracycline on progression of Pseudomonas aeruginosa keratitis in the rabbit. Ann Ophthalmol. 1990;22:179-183.
58. Barletta JP, Balch KC, Dimova HG, et al. Inhibition of Pseudomonas corneal ulcers by a synthetic matrix metalloproteinase inhibitor Galardin; Lt MPI (GM6001). ARVO Abstracts. Invest Ophthalmol Vis Sci. 1993;34:1058.