Microdissection as a means to verify allelic imbalance in tumour biology samples

Anticancer Research

Volumn 16, Issue 1, 1996, Pages 461-464

  Speiser, Paul ^a,b   Gharehbaghi Schnell, Elisabeth ^a   Schneeberger, Christian ^a   Eder, Sabine ^a   Zeillinger, Robert ^a  

^a UNIVERSITY OF VIENNA   (Austria)

^b MEDICAL UNIVERSITY OF VIENNA   (Austria)

Author keywords

AFM051xd10; Boiling method; DNA extraction; Germline mutation; Loss of heterozygosity; Microdissection; TP53 Au i1

Indexed keywords

DNA; MICROSATELLITE DNA; PROTEIN P53;

ADULT; ALLELE; ARTICLE; CONTROLLED STUDY; DISSECTION; DNA DETERMINATION; HEATING; HUMAN; HUMAN CELL; MALIGNANT FIBROUS HISTIOCYTOMA; MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TUMOR CELL;

ALLELES; BASE SEQUENCE; DISSECTION; DNA; DNA, NEOPLASM; EXONS; GENE DELETION; GENES, P53; HETEROZYGOTE; HISTIOCYTOMA, BENIGN FIBROUS; HUMANS; LYMPHOCYTES; MALE; MIDDLE AGED; MOLECULAR SEQUENCE DATA; MUTATION; PARAFFIN EMBEDDING; POLYMORPHISM, GENETIC; REFERENCE VALUES;

EID: 0029871883     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Lane DP: p53, guardian of the genome. Nature 358: 15-16, 1992.
2. Hollstein M, Rice K, Greenblatt MS, Soussi T, Fuchs R, Sorlie T, Hovig E, Smith Sorensen B, Montesano R and Harris CC: Database of p53 gene somatic mutations in human tumours and cell lines. Nucleic Acids Res 22: 3551-3555, 1994.
3. Birch JM, Hartley AL, Tricker KJ, Prosser J, Condie A, Kelsey AM, Harris M, Jones PH, Binchy A, Crowther D, Craft AW, Eden OB, Evans GR, Thompson E, Mann JR, Martin J, Mitchell ELD and Santibanez-Koref MF: Prevalence and diversity of constitutional mutations in the p53 gene among 21 Li-Fraumeni families. Cancer Res 54: 1298-1304, 1994.
4. Knudson AG Jr: Hereditary cancer, oncogenes, and antioncogenes. Cancer Res 45: 437-1443, 1985.
5. Vogelstein B, Fearon ER, Hamilton SR, Kern SE, Preisinger AC, Leppert M, Nakamura Y, White R, Smits AMM and Bos JL: Genetic alterations during colorectal tumour development. N Engl J Med 319: 525-532, 1988.
6. Zhuang Z, Merino MJ, Chuaqui R, Liotta LA and Emmert-Buck MR: Identical allelic loss on chromosome 11q13 in microdissected in situ and invasive human breast cancer. Cancer Res 55: 67-471, 1995.
7. Dockhom-Dworniczak B, Poremba C, Dantcheva R, Stucker A, Brommelkamp E, Blasius S, Mellin W, Roessner A, Yandell DW and Bocker WR: Rapid detection of loss of heterozygosity of chromosome 17p by polymerase chain reaction-based variable number of tandem repeat analysis and detection of single-strand conformation polymorphism of intragenic p53 polymorphisms. Virchows Arch 424: 337-342, 1994.
8. Miller SA, Dykes DD and Polesky HG: Salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16: 1215, 1988.
9. Jones M and Nakamura Y: Detection of loss of heterozygosity at the human TP53 locus using a dinucleotide repeat polymorphism. Gene Chrom Cancer 5: 89-90, 1992.
10. Gyapay G, Morisette J, Vignal A, Dib C, Fizames C, Millasseau P, Marc S, Bernardi G, Lathrop M and Weissenbach J: The 1993 - 94 Genethon human genetic linkage map. Nat Genet 7: 246-331, 1994.
11. Futreal PA, Barrett JC and Wiseman RW: An alu polymorphism intragenic to the TP53 gene. Nucleic Acids Res 19: 6977, 1991.