Partner switching mechanisms in inactivation and rejuvenation of Escherichia coli DNA gyrase by F plasmid proteins LetD (CcdB) and LetA (CcdA)

Journal of Molecular Biology

Volumn 256, Issue 3, 1996, Pages 473-482

  Maki, Satoko ^a,b   Takiguchi, Souichi ^a,c   Horiuchi, Tadao ^a,d   Sekimizu, Kazuhisa ^a   Miki, Takeyoshi ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c NATIONAL KYUSHU CANCER CENTER   (Japan)

^d SOKA UNIVERSITY   (Japan)

Author keywords

DNA gyrase; DNA supercoiling; Escherichia coli; F plasmid; Protein protein interaction

Indexed keywords

BACTERIAL ENZYME; BACTERIAL PROTEIN; DNA TOPOISOMERASE (ATP HYDROLYSING);

ARTICLE; COMPLEX FORMATION; ENZYME INACTIVATION; ESCHERICHIA COLI; MOLECULAR WEIGHT; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SUCROSE GRADIENT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0029871378     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0102     Document Type: Article

References (32)

1. Adams, D. E., Shekhtman, E. M., Zechiedrich, E. L., Schmid, M. B. & Cozzarelli, N. R. (1992). The role of topoisomerase IV in partitioning bacterial replicons and the structure of catenated intermediates in DNA replication. Cell, 71, 277-288.
2. Bernard, P. & Couturier, M. (1992). Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes. J. Mol. Biol. 226, 735-745.
3. Bernard, P., Kézdy, K. E., Melderen, L. V., Steyaert, J., Wyns, L., Pato, M. L., Higgins, P. N. & Couturier, M. (1993). The F plasmid CcdB protein induces efficient ATP-dependent DNA cleavage by gyrase. J. Mol. Biol. 234, 534-541.
4. Bex, F., Karoui, H., Rokeach, L., Drèze, P., Garcia, L. & Couturier, M. (1983). Mini-F encoded proteins: identification of a new 10.5 kilodalton species. EMBO J. 2, 1853-1861.
5. Bliska, J. B. & Cozzarelli, N. R. (1987). Use of site-specific recombination as a probe of DNA structure and metabolism in vivo. J. Mol. Biol. 194, 205-218.
6. Bolivar, F., Rodriguez, R. L., Betlach, M. V. & Boyer, H. W. (1977). Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9. Gene, 2, 75-93.
7. Bradford, M. M. (1976). A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
8. Hussain, K., Begg, K. J., Salmond, G. P. C. and Donachie, W. D. (1987a). ParD: a new gene coding for a protein required for chromosome partitioning and septum localization in Escherichia coli. Mol. Microbiol. 1, 73-81.
9. Hussain, K., Elliott, E. J. and Salmond, G. P. C. (1987b). The ParD-mutant of Escherichia coli also carries a gyrAam mutation. The complete sequence of gyrA. Mol. Microbiol. 1, 259-273.
10. Jaffé, A., Ogura, T. & Hiraga, S. (1985). Effects of the ccd function of the F plasmid on bacterial growth. J. Bacteriol. 163, 841-849.
11. Karoui, H., Bex, F., Drèze, P. & Couturier, M. (1983). Ham22, mini-F mutation which is lethal to host cell and promotes recA-dependent induction of lambdoid prophage. EMBO J. 2, 1863-1868.
12. Kato, J., Nishimura, Y. and Shapiro, B. (1989). Escherichia coli parA is an allele of the gyrB gene. Mol. Gen. Genet. 217, 178-181.
13. Kato, J., Nishimura, Y., Imamura, R., Niki, H., Hiraga, S. & Suzuki, H. (1990). New topoisomerase essential for chromosome segregation in E. coli. Cell, 63, 393-404.
14. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
15. Liu, L. F. & Wang, J. M. (1987). Supercoiling of the DNA template during transcription. Proc. Natl Acad. Sci. USA, 84, 7024-7027.
16. Maki, S. & Kornberg, A. (1988). DNA polymerase III holoenzyme of Escherichia coli. I. Purification and distinctive functions of subunits, τ and γ, the dnaZX gene products. J. Biol. Chem. 263, 6547-6554.
17. Maki, S., Takiguchi, S., Miki, T. & Horiuchi, T. (1992). Modulation of DNA supercoiling activity of Escherichia coli DNA gyrase by F plasmid proteins. Antagonistic actions of LetA (CcdA) and LetD (CcdB) proteins. J. Biol. Chem. 267, 12244-12251.
18. Marians, K. J. (1987). DNA gyrase-catalyzed decatenation of multiply linked DNA dimers. J. Biol. Chem. 262, 10362-10368.
19. Menzel, R. & Gellert, M. (1987). Fusions of the Escherichia coli gyrA and gyrB control regions to the galactokinase gene are inducible by coumermycin treatment. J. Bacteriol. 169, 1272-1278.
20. Miki, T., Yoshioka, K. & Horiuchi, T. (1984a). Control of cell division by sex factor F in Escherichia coli. I. The 42.84-43.6 F segment couples cell division of the host bacteria with replication of plasmid DNA. J. Mol. Biol. 174, 605-625.
21. Miki, T., Chang, Z.-T. & Horiuchi, T. (1984b). Control of cell division by sex factor F in Escherichia coli. II. Identification of genes for inhibitor protein and trigger protein on the 42.84-43.6 F segment. J. Mol. Biol. 174, 626-646.
22. Miki, T., Orita, T., Furuno, M. & Horiuchi, T. (1988). Control of cell division by sex factor F in Escherichia coli. III. Participation of the groES (mopB) gene of the host bacteria. J. Mol. Biol. 201, 327-338.
23. Miki, T., Park, J. A., Nagao, K., Murayama, T. & Horiuchi, T. (1992). Control of segregation of chromosomal DNA by sex factor F in Escherichia coli. Mutants of DNA gyrase subunit A suppress letD (ccdB) product growth inhibition. J. Mol. Biol. 225, 39-52.
24. Mizuuchi, K., Mizuuchi, M., O'Dea, M. H. & Gellert, M. (1984). Cloning and simplified purification of Escherichia coli DNA gyrase A and B proteins. J. Biol. Chem. 259, 9199-9201.
25. Murayama, N., Shimizu, H., Takiguchi, S., Baba, Y., Amino, H., Horiuchi, T., Sekimizu, K. & Miki, T. (1996). Evidence for involvement of Escherichia coli genes pmbA, csrA and a previously unrecognized gene, tldD, in the control of DNA gyrase by letD(ccdB) of sex factor F. J. Mol. Biol. 256, 483-502.
26. Ogura, T. & Hiraga, S. (1983). Mini-F plasmid genes that couples host cell division to plasmid proliferation. Proc. Natl Acad. Sci. USA, 80, 4784-4788.
27. Sigel, L. M. & Monty, K. J. (1966). Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases. Biochim. Biophys. Acta, 112, 346-362.
28. Sommer, S., Bailone, A. & Devoret, R. (1985). SOS induction by thermosensitive replication mutants of mini-F plasmid. Mol. Gen. Genet. 198, 456-464.
29. Steck, T. R. & Drlica, K. (1984). Bacterial chromosome segregation: evidence for DNA gyrase involvement in decatenation. Cell, 36, 1081-1088.
30. Tam, J. E. & Kline, B. C. (1989). The F plasmid ccd autorepressor is a complex of CcdA and CcdB proteins. Mol. Gen. Genet. 219, 26-32.
31. Van Melderen, L., Bernard, P. & Couturier, M. (1994). Lon-dependent proteolysis of CcdA is the key control for activation of CcdB in plasmid-free segregant bacteria. Mol. Microbiol. 11, 1151-1157.
32. Vosberg, H.-P. (1985). DNA topoisomerases: enzymes that control DNA conformation. Curr. Top. Microbiol. Immunol. 114, 19-102.