An NMR method to identify nondestructively chemical compounds bound to a single solid-phase-synthesis bead for combinatorial chemistry applications

Journal of the American Chemical Society

Volumn 118, Issue 9, 1996, Pages 2305-2306

  Sarkar, Susanta K ^a   Garigipati, Ravi S ^a   Adams, Jerry L ^a   Keifer, Paul A ^b  

^a GLAXOSMITHKLINE   (United Kingdom)

^b Varian Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BENZOIC ACID DERIVATIVE;

ARTICLE; DRUG BINDING; DRUG STRUCTURE; NUCLEAR MAGNETIC RESONANCE; PROTEIN SYNTHESIS; TECHNIQUE;

EID: 0029871215     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja952434d     Document Type: Article

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35. 13C-labeled single-bead samples used in this study. The Nanoprobe sample cell restricts 100% of the sample (40 μL maximum) within the active region of the receiver coil to ensure that even a single bead would always be detected.
36. 100-200 mesh Merrifield resin obtained from Calbiochem was functionalized according to: Wang, S. S. J. Am. Chem. Soc. 1973. 95, 1328-1333. The beads selected for NMR were measured by a light microscope to have a 100 μm diameter.
37. 13C correlation and is presumably absorbed water.
38. The stated loading of 1.4 mmol/g and an estimated 1 700 000 beads/ g. gives ∼800 pmol of sample per bead.
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