Design of a novel bicistronic expression vector with demonstration of a p16INK4-induced G1-S block

Cancer Research

Volumn 56, Issue 8, 1996, Pages 1719-1723

  Poulos, Nicholas E ^a,b   Farmer, Andrew A ^a,c   Chan, Kate W K ^a   Stanbridge, Eric J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LERNER RESEARCH INSTITUTE   (United States)

^c UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; CD20 ANTIGEN; INTESTINE ENZYME; ISOENZYME;

ARTICLE; CELL CYCLE G1 PHASE; CELL CYCLE S PHASE; CONTROLLED STUDY; CYTOTOXICITY; EUKARYOTE; EXPRESSION VECTOR; GENE EXPRESSION; GENETIC TRANSFECTION; GLIOBLASTOMA; HUMAN; HUMAN CELL; PRIORITY JOURNAL;

ALKALINE PHOSPHATASE; CARRIER PROTEINS; CELL CYCLE; CELL LINE; CYCLIN-DEPENDENT KINASE INHIBITOR P16; CYTOMEGALOVIRUS; ELECTROPORATION; ENHANCER ELEMENTS (GENETICS); ENZYME INHIBITORS; G1 PHASE; GENE EXPRESSION; GENES; GENETIC VECTORS; GLIOBLASTOMA; HUMANS; KINETICS; PROTEIN BIOSYNTHESIS; RECOMBINANT PROTEINS; RESTRICTION MAPPING; S PHASE; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0029870611     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Gossen, M., and Bujard, H Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA, 89. 5547-5551, 1992.
2. Van den Heuvel, S., and Harlow, E. Distinct roles for cyclin-dependent kinases in cell cycle control. Science (Washington DC), 262: 2050-2054, 1993.
3. Stamenkovic, L, and Seed, B. Analysis of two cDNA clones encoding the lymphocyte antigen cD20, a type III integral membrane protein. J. Exp. Med., 167: 1975-1980, 1988.
4. Koh, J , Enders, G. H., Dynlacht, B D., and Harlow, E Tumor-derived p16 alleles encoding proteins defective in cell-cycle inhibition. Nature (Lond), 375: 506-510, 1995
5. Henthorn, P S , Raducha, M., Kadesch, T , Mitchell, J W , and Harris, H Sequence and characterization of the human intestinal alkaline phosphatase gene J Biol. Chem., 263: 12011-12019, 1988
6. Millán, J. L. Oncodevelopmental expression and structure of alkaline phosphatase genes Anticancer Res., 8: 995-1004, 1988.
7. Low, M L Biochemistry of the glycosyl-phosphatidylinositol membrane protein anchors. Biochem. J., 244: 1-13, 1987
8. Alpers, D. H., Eliakim, R., and DeSchryver-Kecskemeti, K. Secretion of hepatic and intestinal alkaline phosphatase: similarities and differences Clin Chim. Acta, 186: 211-224, 1989
9. Latham, K. M , and Stanbridge, E. J. Identification of the HeLa tumor-associated antigen, p75/150, as intestinal alkaline phosphatase and evidence for its transcriptional regulation. Proc. Natl. Acad. Sci. USA, 87: 1263-1267, 1990.
10. Bicknell, D. C , Sutherland, D. R., Stanbridge, E J., and Greaves, M. F. Monoclonal antibodies specific for a tumor-associated membrane phosphoprotein in human cell hybrids. Hybridoma, 4, 143-152, 1985.
11. Parks, G. D., Duke, G M , and Palmenberg, A C. Encephalomyocarditis virus 3C protease: efficient cell-free expression from clones which link viral 5′ noncoding sequences to the P3 region. J. Virol., 60: 376-384, 1986.
12. Elroy-Stein, O., Fuerst, T R , and Moss, B. Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5′ sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system Proc. Natl Acad. Sci USA, 86. 6126-6130, 1989.
13. Kamb, A., Gruis, N A., Weaver-Feldhaus, J., Liu, Q., Harshman, K , Tavtigian, S V., Stockert, E., Day, R. S., III, Johnson, B. E , and Skolnik, M. H A cell cycle regulator potentially involved in genesis of many tumor types Science (Washington DC), 264 436-440, 1994.
14. Dolbeare, F , and Gray, J W. Use of restriction endonucleases and exonuclease III to expose halogenated pyrimidines for immunochemical staining. Cytometry, 9 631-635, 1988
15. Takagi, S., McFadden, M L , Humphreys, R. E., Woda, B A , and Sairenji, T. Detection of 5-bromo-2-deoxyuridine (BrdUrd) incorporation with monoclonal anti-BrdUrd antibody after deoxyribonuclease treatment. Cytometry, 14, 640-648, 1993.
16. Schmid, I., Uittenbogaart, C H , and Giorgi, J V. A gentle fixation and permeabilization method for combined cell surface and intracellular staining with improved precision in DNA quantification Cytometry, 12: 279-285, 1991