Purification and characterization of three larval excretory-secretory proteins of Dirofilaria immitis

Molecular and Biochemical Parasitology

Volumn 75, Issue 2, 1996, Pages 221-229

  Frank, G R ^a   Grieve, R B ^a  

^a Paravax Inc ^*  (United States)

Author keywords

Antigens; Dirofilaria; Excretory secretory; Filariasis; Molt

Indexed keywords

PARASITE ANTIGEN; VACCINE;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DIROFILARIA IMMITIS; DOG; EXCRETION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LARVA; MOLTING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; SECRETION; VACCINATION;

ANIMALIA; CANIS FAMILIARIS; DIROFILARIA; DIROFILARIA IMMITIS;

EID: 0029870523     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-6851(95)02533-2     Document Type: Article

References (12)

1. R.B. Grieve D. Abraham M. Mika-Grieve B.P. Seibert Induction of protective immunity in dogs to infection with Dirofilaria immitis using chemically-abbreviated infections Am. J. Trop. Med. Hyg. 39 1988 373 379
2. J.S. Mejia C.K. Carlow An analysis of the humoral immune response of dogs following vaccination with irradiated infective larvae of Dirofilaria immitis Parasite Immunol. 16 1994 157 164
3. M.M. Wong M.F. Guest M.M.J. Lavoipierre Dirofilaria immitis : fate and immunogenicity of irradiated infective stage larvae in beagles Exp. Parasitol. 35 1974 465 474
4. G.R. Frank R.B. Grieve Metabolic labeling of Dirofilaria immitis third- and fourth-stage larvae and their excretory-secretory products J. Parasitol. 77 1991 950 956
5. K.L. Stone M.B. Lopresti J.M. Crawford R. Deangelis K.R. Williams Enzymatic digestion of proteins and HPLC peptide isolation P.T. Matsudaira A Practical Guide to Protein and Peptide Purification for Microsequencing 1989 Academic Press, Inc San Diego, CA 31 47
6. H. Schagger G. Von Jagow Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa Anal. Biochem. 166 1987 368 379
7. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
8. N. Legendre P. Matsudaira Purification of proteins and peptides by SDS-PAGE P.T. Matsudaira A Practical Guide to Protein and Peptide Purification for Microsequencing 1989 Academic Press, Inc San Diego, CA 49 69
9. R.B. Grieve G.R. Frank M. Mika-Grieve J.A. Culpepper M. Mok Identification of Dirofilaria immitis larval antigens with immunoprophylactic potential using sera from immune dogs J. Immunol. 148 1992 2511 2515
10. R.M. Hewick M.W. Hunkapiller L.E. Hood W.J. Dreyer A gas-liquid solid phase peptide and protein sequenator J. Biol. Chem. 256 1981 7990 7997
11. M.J. Geisow A. Aitken Gas- or pulsed liquid-phase sequence analysis J.B.C. Findlay M.J. Geisow Protein Sequencing: A Practical Approach 1989 IRL Press Oxford, England 85 98
12. W.F. Patton N. Chung-Welch M.F. Lopez R.P. Cambria B.L. Utterback W.M. Skea Tris-tricine and tris-borate buffer systems provide better estimates of human mesothelial cell intermediate filament protein molecular weights than the standard tris-glycine system Anal. Biochem. 197 1991 25 33