Gender determination of forensic samples using PCR amplification of ZFX/ZFY gene sequences

Journal of Forensic Sciences

Volumn 41, Issue 2, 1996, Pages 279-286

  Reynolds, Rebecca ^a   Varlaro, Joseph ^a  

^a ROCHE MOLECULAR SYSTEMS   (United States)

Author keywords

forensic science; gender determination; polymerase chain reaction (PCR); reverse dot blot

Indexed keywords

DNA; HLA DQ ANTIGEN; ZINC FINGER PROTEIN;

ARTICLE; DNA DETERMINATION; FEMALE; FORENSIC IDENTIFICATION; GENE LOCUS; GENE SEQUENCE; HUMAN; HUMAN CELL; ISOLATION PROCEDURE; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SEX DIFFERENCE;

EID: 0029869403     PISSN: 00221198     EISSN: None     Source Type: Journal    
DOI: 10.1520/jfs15427j     Document Type: Article

References (22)

1. Jeffreys AJ, Wilson V, Thein SL. Hypervariable 'minisatellite' regions in human DNA. Nature 1985;314:67-73.
2. Budowle B, Waye JS, Shutler GG, Baechtel SF. Haelll-a suitable restriction endonuclease for restriction fragment length polymorphism analysis of biological evidence samples. J Forensic Sci 1990;35:530-36.
3. Saiki R, Scharf S, Faloona F, Mullis K, Horn G, Erlich HA, Arnheim N. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 1985;230:1350-54.
4. von Beroldingen CH, Blake ET, Higuchi R, Sensabaugh GH, Erlich HA. Applications of PCR to the analysis of biological evidence. PCR technology, principles and amplifications for DNA amplification. Erlich H.A., editor, Stockton Press, Inc., New York, NY, 1989;203-23.
5. Reynolds R, Sensabaugh G, Blake E. Analysis of genetic markers in forensic DNA samples using the polymerase chain reaction. Analyt Chem 1991;63:1-14.
6. Saiki RK, Walsh PS, Levenson CH, Erlich HA. Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes. Proc National Acad Sci, USA 1989;86:6230-34.
7. Sajantila A, Strom M, Budowle B, Karhunen PJ, Peltonen L. The polymerase chain reaction and post-mortem forensic identity testing: application of amplified D1S80 and HLA-DQ alpha loci to the identification of fire victims. Forensic Sci Int 1991;51:23-34.
8. Blake E, Mihalovich J, Higuchi R, Walsh PS, Erlich H. Polymerase chain reaction (PCR) amplification and human leukocyte antigen (HLA)-DQα oligonucleotide typing on biological evidence samples: casework experience. J Forensic Sci 1992;37:700-26.
9. Comey CT, Budowle B, Adams DE, Baumstark AL, Lindsey JA, Presley LA. PCR amplification and typing of the HLA DQα gene in forensic samples. J Forensic Sci 1993;38:239-49.
10. Budowle B, Lindsey JA, DeCou JA, Koons BW, Giusti AM, Comey CT. Validation and population studies of the loci LDLR, GYPA, HBGG, D7S8, and GC (PM Loci), and HLA-DQα using a multiplex amplification and typing procedure. J Forensic Sci 1995;40:45-54.
11. Herrin G Jr, Fildes N, Reynolds R. Evaluation of the AmpliType® PM DNA test system on forensic case samples. J Forensic Sci 1994;39(5):1247-53.
12. Hochmeister MN, Budowle B, Borer UV, Eggmann U, Comey CT, Dimhofer R. Typing of deoxynucleic acid (DNA) extracted from compact bone from human remains. J Forensic Sci 1991;36:1649-61.
13. Wiegand P, Budowle B, Rand S, Brinkmann B. Forensic validation of the STR systems SE33 and TC11. Int J Legal Med 1993;105:315-20.
14. Whitaker J, Clayton TM, Urquhart AJ, Millican ES, Downes TJ, Kimpton CP, Gill P. Short tandem repeat typing of bodies from a mass disaster: high success rate and characteristic amplification patterns in highly degraded samples. BioTechniques 1995;18: 670-77.
15. Stacks B, Witte MM. Sex determination of dried blood stains using the polymerase chain reaction (PCR) with homologous X-Y primers of the zinc finger protein gene. J Forensic Sci 1996;41(2):287-90.
16. Schneider-Gädicke A, Beer-Romero P, Brown LG, Nussbaum R, Page DC. ZFX has a gene structure similar to ZFY, the putative human sex determinant, and escapes X inactivation. Cell 1989;57:1247-58.
17. Page D, Mosher R, Simpson E, Fisher E, Mardon G, Pollack J, McGillivary B, de la Chapelle A, Brown L. The sex-determining region of the human Y chromosome encodes a finger protein. Cell 1987;51:1091-1104.
18. Sullivan KM. A rapid and quantitative DNA sex test: fluorescence-based PCR analysis of X-Y homologous gene amelogenin. BioTechniques 1993;15(4):637-41.
19. Miller SA, Dykes DD, Polesky HE A simple salting out procedure for extracting DNA from human nucleated cells. Nucl Acids Res 1988;6:1215.
20. Walsh PS, Varlaro J, Reynolds R. A rapid chemiluminescent method for quantitation of human DNA. Nucl Acids Res 1992;20:5061-65.
21. Perkin-Elmer, AmpliType® User Guide, Version 2.
22. Aasen E, Medrano JF. Amplification of the ZFY and ZFX genes for sex identification in humans, cattle, sheep and goats. Bio/Technology 1990;8:1279-80.