Simplified RT/PCR quantitation of gene transcripts in cultured neuroblastoma (SN49) and microglial (BV-2) cells using capillary electrophoresis and laser-induced fluorescence

Journal of Neuroscience Methods

Volumn 65, Issue 1, 1996, Pages 77-91

  Personett, David A ^a   Chouinard, Michael ^a   Sugaya, Kiminobu ^a   McKinney, Michael ^a  

^a MAYO CLINIC   (United States)

Author keywords

Cell line; Electrophoresis; Message RNA quantitation; Polymerase chain reaction

Indexed keywords

COMPLEMENTARY DNA; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MESSENGER RNA; NEUROMODULIN; NITRIC OXIDE SYNTHASE;

ANIMAL CELL; ARTICLE; CAPILLARY ELECTROPHORESIS; CELL CULTURE; CONTROLLED STUDY; IN SITU HYBRIDIZATION; MICROGLIA; NEUROBLASTOMA; NONHUMAN; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

EID: 0029869286     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/0165-0270(95)00150-6     Document Type: Article

References (51)

1. Baba, Y., Tomisaki, R., Sumita, C., Tsuhako, M., Miki, t., and Ogihara, T. (1994) High-resolution separation of PCR products and gene diagnosis by capillary gel electrophoresis, Biomed. Chromatog., 8: 291-293.
2. Becker-Andre, M. and Hahlbrock, K. (1989) Absolute mRNA quantification using the polymerase chain reaction (PCR): a novel approach by a PCR aided transcript titration assay (PATTY), Nucl. Acids Res., 17: 9437-9446.
3. Bocchini, v., Mazzolla, R., Barluzzi, R., Blasi, E., Sick, P., and Kettenmann, H. (1992) An immortalized cell line expresses properties of activated microglial cells, J. Neurosci. Res., 31: 616-621.
4. Boje, K.M. and Arora, P.K. (1992) Microglial-produced nitric oxide and reactive nitrogen oxides mediate neuronal cell death, Brain Res., 587: 250-256.
5. Bouaboula, M., Legoux, P., Pessegue, B., Delpech, B., Dumont, X., Piechaczyk, Casellas, P., and Shire, D, (1992) Standardization of mRNA titration using a polymerase chain reaction method involving co-amplification with a multispecific internal control, J. Biol. Chem., 267: 21830-21838.
6. Chao, C.C., Hu, S., Molitor, T.W., Shaskan, E.G., and Peterson, P.K. (1992) Activated microglia mediate neuronal cell injury via a nitric oxide mechanism, J. Immunol. 149: 2736-2741.
7. Chelly, J., Kaplan, J.-C., Maire, P., Gautron, S., and Kahn, A. (1988) Transcription of the dystrophin gene in human muscle and non-muscle tissues, Nature, 333: 858-860.
8. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction, Anal. Biochem., 162: 156-159.
9. Cohen, A.S., Najarian, D., Smith, J.A. and Karger, B.L. (1988) Rapid separation of DNA restriction fragments using capillary electrophoresis, J. Chromatog., 458: 323-333.
10. Colton, C.A., Snell, J., Chemyshev, O., and Gilbert, D.L. (1994) Induction of superoxide and nitric oxide production in cultured microglia, Ann. NY Acad. Sci., 738: 54-63.
11. Corradin, S.B., Mauel, J., Donini, S.D., Quattrocchi, E., and Ricciardi-Castagnoli, P. (1993) Inducible nitric oxide synthase activity of cloned murine microglial cells, Glia, 7: 255-262.
12. Dawson, V.L., Brahmbhatt, H.P., Mong, J.A., and Dawson, T.M. (1994) Expression of inducible nitric oxide synthase causes delayed neurotoxicity in primary mixed neuronal-glial cortical cultures, Neuropharmacology, 33: 1425-1430.
13. Dostal, D.E., Rothblum, K.N., and Baker, K.M. (1994) An improved method for absolute quantification of mRNA using multiplex polymerase chain reaction: determination of renin and angiotensinogen mRNA levels in various tissues, Anal. Biochem., 223: 239-250.
14. Endoh, M., Maiese, K., and Wagner, J. (1994) Expression of the inducible form of nitric oxide synthase by reactive astrocytes after transient global ischemia, Brain Res., 651: 92-100.
15. Fasco, M.J., Treanor, C.P., Spivack, S., Figge, H.L., and Kaminsky, L.S. (1995) Quantitative RNA-polymerase chain reaction-DNA analysis by capillary electrophoresis, Anal. Biochem., 224: 149-147.
16. Galea, E. and Feinstein, D.L. (1992) Rapid synthesis of DNA deletion constructs for mRNA quantitation: analysis of astrocyte mRNAs, PCR Meth. Appl., 2: 66-69.
17. Galea, E., Reis, D.J., and Feinstein, D.L. (1994) Cloning and expression of inducible nitric oxide synthase from rat astrocytes, J. Neurosci. Res., 37: 406-414.
18. Gilliland, G., Perrin, S., Blanchard, K., and Bunn, H.F., (1990) Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction, Proc. Natl. Acad. Sci. USA, 87: 2725-2729.
19. Grassi, G., Zentilin, L., Tafuro, S., Diviacco, S., Ventura, A., Falaschi, A., and Giacca, M. (1994) A rapid procedure for the quantitation of low abundance RNAs by competitive reverse transcription-polymerase chain reaction, Nucl. Acids Res., 22: 4547-4549.
20. 15N]nitrate in biological fluids. Anal. Biochem., 126: 131-138.
21. Hastie, N.D. and Bishop, J.O. (1976) The expression of three abundance classes of messenger RNA in mouse tissues, Cell, 9: 761-774.
22. Hewett, S.J., Csernansky, C.A., and Choi, D.W. (1994) Selective potentiation of NMDA-induced neuronal injury following induction of astrocytic i-NOS, Neuron, 13: 487-494.
23. Iadecola, C., Xu, X., Zhang, F., El-Fakahany, E.E., and Ross, M.E. (1995) Marked induction of calcium-independent nitric oxide synthase activity after focal cerebral ischemia, J. Cereb. Blood Flow Metab., 15: 52-59.
24. Karger, A.E., Harris, J.E. and Gesteland, R.F. (1991) Capillary electrophoresis with laser-induced fluorescence detection of PCR fragments using thiazole orange, Nucl. Acids Res., 19: 4955-4962.
25. Kuypers, A.W.H.M., Meijerink, J.P.P. Smetsers, T.F.C.M., Linssen, P.C.M., and Mensink, E.J.B.M. (1994) Quantitative analysis of DNA aberrations amplified by competitive polymerase chain reaction using capillary electrophoresis, J. Chromatog. B, 660: 271-277.
26. Krug, M.S. and Berger, S.L. (1987) First-strand cDNA synthesis primed with oligo(dT), Meth. Enzymol., 152: 316-325.
27. Lu, W., Han, D.-S., Yuan, J., and Andrieu, J.-M. (1994) Multi-target PCR analysis by capillary electrophoresis and laser-induced fluorescence, Nature, 238: 269-271.
28. Marino, M.A., Turni, L.A., Del Rio, S.A., and Williams, P.E. (1994) Molecular size determinations of DNA restriction fragments and polymerase chain reaction products using capillary gel electrophoresis, J. Chromatog. A, 676: 185-189.
29. McKinney, M. and Robbins, M. (1992) Chronic atropine administration up-regulates rat cortical muscarinic m1 receptor mRNA molecules: assessment with the RT/PCR, Mol. Brain Res., 12: 39-45.
30. Merrill, J.E., Ignarro, L.J., Sherman, M.P., Melinek, J., and Lane, T.E. (1993) Microglial cell cytotoxicity of oligodendrocytes is mediated through nitric oxide, J. Immunol., 151: 2132-2141.
31. Morrison, C. and Gannon, F. (1994) The impact of the PCR plateau phase on quantitative PCR, Biochim. Biophys. Acta, 1219: 493-498.
32. Murphy, S., Simmons, M.L., Agullo, L., Garcia, A., Feinstein, D.L., Galea, E., Reis, D.J., Minc-Golomb, D., and Schwartz, J.P. (1993) Synthesis of nitric oxide in CNS glial cells, Trends Neurosci., 16: 323-328.
33. Nedelman, J., Heagerty, P., and Lawrence, C. (1992) Quantitative PCR with internal controls, CABIOS, 8: 65-70.
34. Pannetier, C., Delassus, S., Darche, S., Saucier, C., and Kourilsky, P. (1993) Quantitative titration of nucleic acids by enzymatic amplification reactions run to saturation, Nucl. Acids Res., 21: 577-583.
35. Peterson, P.K., Hu, S., Anderson, W.R., and Chao, C.C. (1994) Nitric oxide production and neurotoxicity mediated by activated microglia from human versus mouse brain, J. Infect. Dis., 170: 457-460.
36. Robbins, M. and McKinney, M. (1992) Transcriptional regulation of neuromodulin (GAP-43) in mouse neuroblastoma clone N1E-115 as evaluated by the RT/PCR method, Mol. Brain Res., 13: 83-92.
37. Rossomando, E.F., White, L., and Ulfelder, K.J. (1994) Capillary electrophoresis: separation and quantitation of reverse transcriptase polymerase chain reaction products from polio virus, J. Chromatog. B, 656: 159-168.
38. Santagati, S., Bettini, E., Asdente, M., Muramatsu, M., and Maggi, A. (1993) Theoretical considerations for the application of competitive polymerase chain reaction to the quantitation of a low abundance mRNA: estragen receptor. Biochem. Pharmacol., 46: 1797-803.
39. Schwartz, H.E. and Ulfelder, K.J. (1992) Capillary electrophoresis with laser-induced fluorescence detection of PCR fragments using thiazole orange, Anal. Chem., 64: 1737-1740.
40. Siebert, P.D. and Larrick, J.W. (1992) Competitive PCR, Nature, 359: 557-558.
41. Skaper, S.D., Facci, L., and Leon, A. (1995) Inflammatory mediator stimulation of astrocytes and meningeal fibroblasts induces neuronal degeneration via the nitridergic pathway, J. Neurochem., 64: 266-276.
42. +-ATPase, Anal. Biochem., 217: 91-97.
43. Stuehr, D.J. and Nathan, C.F. (1989) Nitric oxide: a macrophage product responsible for cytostasis and respiratory inhibition in tumor target cells. J. Exp. Med., 169: 1543-1555.
44. Sugaya, K. and McKinney, M. (1994) Nitric oxide synthase gene expression in cholinergic neurons in the rat brain examined by combined immunocytochemistry and in situ hybridization histochemistry, Mol. Brain Res., 23: 111-125.
45. Ulfelder, K.J., Schwartz, H.E., Hall, J.M. and Sunzeri, F.J. (1992) Restriction fragment length polymorphism analysis of ERBB2 oncogene by capillary electrophoresis, Anal. Biochem., 200: 260-267.
46. Valeninis, B., Zaffaroni, N., Sturani, E., Martegani, E., and Silvestrini, R. (1993) Detection of DNA polymerase-β gene expression by competitive polymerase chain reaction in human ovarian carcinoma cells, Anticancer Res., 13: 125-128.
47. Volkenandt, M., Dicker, A.P., Banerjee, D., Fanin, R., Schweitzer, B., Horikoshi, T., Danenberg, K., Danenberg, P., and Bertino, J.R. (1992) Quantitation of gene copy number and mRNA using the polymerase chain reaction, Proc. Soc. Exp. Biol. Med., 200: 1-6.
48. Wang, A.M., Doyle, M.V. and Mark, F. (1989) Quantitation of mRNA by the polymerase chain reaction, Proc. Natl. Acad Sci. USA, 86: 9717-9721.
49. Williams, P.E., Marino, M.A., Del Rio, S.A., Turni, L.A., and Devaney, J.M. (1994) Analysis of DNA restriction fragments and polymerase chain reaction products by capillary electrophoresis, J. Chromatog., 680: 525-540.
50. Wood, P.L., Choksi, S., and Bocchini, V. (1994) Inducible microglial nitric oxide synthase: a large membrane pool, NeuroReport, 5: 977-980.
51. Xie, Q.-W., Cho, H.J., Calaycay, J., Mumford, R.A., Swiderek, K.M., Lee, T.D., Ding, A., Troso, T., and Nathan, C. (1992) Cloning and characterization of inducible nitric oxide from mouse macrophages, Science, 256: 225-228.