Solid phase synthesis and purification of 5′-mercaptoalkylated oligonucleotides

Bioorganic and Medicinal Chemistry Letters

Volumn 6, Issue 6, 1996, Pages 683-688

  Kumar, P ^a   Bhatia, D ^a   Rastogi, R C ^b   Gupta, K C ^a  

^a CSIR INSTITUTE OF GENOMICS AND INTEGRATIVE BIOLOGY   (India)

^b UNIVERSITY OF DELHI   (India)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGONUCLEOTIDE; THIOL DERIVATIVE;

AFFINITY CHROMATOGRAPHY; ARTICLE; DRUG PURIFICATION; DRUG SYNTHESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TECHNIQUE;

EID: 0029868869     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0960-894X(96)00084-4     Document Type: Article

References (11)

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5. Bischoff, R.; Coull, J.M.; Regnier, F.E. Anal. Biochem., 1987, 164, 336.
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8. Kuijpers, W.H.A.; van Boeckel, C.A.A. Tetrahedron, 1993, 49, 10931.
9. Gene Assembler Plus Manual, Uppsala, Sweden.
10. Kumar, P.; Bose, N.K.; Gupta, K.C. Tet. Lett., 1991, 32, 967.
11. HPLC conditions : column, Lichrosphere (Merck) RP-18; gradient, 0-30% solvent B in 30 minutes at a flow rate of 1.0 ml/min. Solvent A - 0.1 M ammonium acclate, pH 7.2, solvent B = Acetonitrile.