Detection and quantisation of specific mRNAs by ribonuclease protection assay using denaturing horizontal polyacrylamide gel electrophoresis: A radioactive and nonradioactive approach

Electrophoresis

Volumn 17, Issue 3, 1996, Pages 471-472

  Plath, Annette ^a   Peters, Friedolf ^b   Einspanier, Ralf ^a,c  

^a Forschungszentrum Milch Lebensm W   (Germany)

^b St Hildegardis Krankenhaus   (Germany)

^c Institute of Physiology   (Germany)

Author keywords

Digoxigenin; Horizontal polyacrylamide gel electrophoresis; Ribonuclease protection assay

Indexed keywords

ELECTROPHORESIS; RADIOACTIVITY;

DIGOXIGENIN; GROWTH FACTOR; HORIZONTAL POLYACRYLAMIDE GEL ELECTROPHORESIS; NONRADIOACTIVE; PHARMACIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTECTION ASSAY; PROTEIN FACTORS; QUANTISATION; RIBONUCLEASE PROTECTION ASSAY; POLYACRYLAMIDE GELS;

GELS;

MESSENGER RNA;

ANIMAL TISSUE; ARTICLE; CATTLE; CONTROLLED STUDY; INTERMETHOD COMPARISON; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; QUANTITATIVE ASSAY; RNA ANALYSIS; TECHNIQUE;

EID: 0029868197     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150170306     Document Type: Article

References (6)

1. Vesterberg, O., Electrophoresis 1993, 14, 1243-1249.
2. Boge, A., Gerstmeier, R., Einspanier, R., Insect Mol. Biol. 1994, 3, 267-271.
3. Turnbow, M. A., Garner, C. W., BioTechniques 1993, 15, 267-270.
4. Wundrack, I., Dooley, S., Electrophoresis 1992, 13, 637-638
5. Chomczynski, P., Sacchi, N., Anal. Biochem. 1987, 62, 156-159.
6. Bassam, B. J., Caetano-Anolles, G., Gresshoff, P. M., Anal. Biochem. 1991, 196, 80-83.