Novel detection method of nitric oxide using horseradish peroxidase

Biological and Pharmaceutical Bulletin

Volumn 19, Issue 4, 1996, Pages 649-651

  Kikuchi, Kazuya ^a   Nagano, Tetsuo ^a   Hirobe, Masaaki ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

difference spectra; horseradish peroxidase; nitric oxide; Soret band

Indexed keywords

FERRIC ION; FERROUS ION; HORSERADISH PEROXIDASE; NITRIC OXIDE;

AQUEOUS SOLUTION; ARTICLE; COMPLEX FORMATION; PHAGOCYTE; PROTEIN STABILITY; SIGNAL TRANSDUCTION; STEREOSPECIFICITY; VASCULAR ENDOTHELIUM;

EID: 0029867796     PISSN: 09186158     EISSN: None     Source Type: Journal    
DOI: 10.1248/bpb.19.649     Document Type: Article

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17. 5.
18. Each one aliquot of NO (0.2 nmol) was 5 μl of sodium phosphate buffer solution (pH 7.4) and HRP solution was 0.1 μM the same buffer. A cuvette with 10 cm path length was used, and the volume of the container was 38 ml.