Chloramphenicol Resistance Interferes with Purification of Histidine-Tagged Fusion Proteins from Recombinant Escherichia coli

Analytical Biochemistry

Volumn 236, Issue 2, 1996, Pages 357-358

  Oswald, Tanja ^a   Rinas, Ursula ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ESCHERICHIA COLI;

FUSION PROTEINS; RECOMBINANT ESCHERICHIA COLI;

RECOMBINANT PROTEINS;

CHLORAMPHENICOL; HISTIDINE; HYBRID PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ANTIBIOTIC RESISTANCE; CONTAMINATION; ESCHERICHIA COLI; GENETIC RECOMBINATION; NOTE; PRIORITY JOURNAL; PROTEIN PURIFICATION; ULTRAFILTRATION;

EID: 0029867660     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1996.0180     Document Type: Article

References (5)

1. Hochuli, E., Bannwarth, W., Döbeli, H., Gentz, R., and Stüber, D. (1988) BioTechnology 6, 1321-1325.
2. Hengen, P. N. (1995) Trends Biochem. Sci. 20, 285-286.
3. Oswald, T., Wende, W., Pingoud, A., and Rinas, U. (1994) Appl. Microbiol. Biotechnol. 42, 73-77.
4. Shaw, W. V., Packman, L. C., Burleigh, B. D., Dell, A., Morris, H. R., and Hartley, B. S. (1979) Nature 282, 870-872.
5. Neidhardt, F. C. (1987) in Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology (Neidhardt, F. C., Ingraham, J. L., Low, K. B., Magasanik, B., Schaechter, M., and Umbarger, H. E., Eds.), Vol. 1, pp. 3-6, Am. Soc. Microbiol., Washington, DC.