Mutations within the first LSGGQ motif of Ste6p cause defects in a-factor transport and mating in Saccharomyces cerevisiae

Journal of Bacteriology

Volumn 178, Issue 6, 1996, Pages 1712-1719

  Browne, Barclay L ^a   McClendon, Vera ^a   Bedwell, David M ^a  

^a University of Alabama at Birmingham   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABC TRANSPORTER; MATING HORMONE ALPHA FACTOR;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL MUTATION; CELLULAR DISTRIBUTION; ENDOCYTOSIS; MATING; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION;

EID: 0029867564     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.6.1712-1719.1996     Document Type: Article

References (51)

1. Ames, G. F.-L., C. S. Mimura, S. R. Holbrook, and V. Shyamala. 1992 Trafic ATPases: a superfamily of transport proteins operating from Escherichia coli to humans. Adv. Enzymol. 65:1-47.
2. Ames, G. F.-L., C. S. Mimura, and V. Shyamala. 1990. Bacterial periplasmic permeases belong to a family of transport proteins operating from E. coli to human: traffic ATPases. FEMS Microbiol. Rev. 75:429-47.
3. Anderegg, R. J., R. Betz, S. A. Carr, J. W. Crabb, and W. Duntze. 1988. Structure of Saccharomyces cerevisiae mating hormone a-factor: identification of S-farnesyl cysteine as a structural component. J. Biol. Chem. 263: 18236-18240.
4. Baffi, R. A., P. Shenbagamurthi, K. Terrance, J. M. Becker, F. Naider, and P. N. Lipke. 1984. Different structure-function relationships for α-factor-induced morphogenesis and agglutination in Saccharomyces cerevisiae. J. Bacteriol. 158:1152-1156.
5. Berkower, C., D. Loayza, and S. Michaelis. 1994. Metabolic instability and constitutive endocytosis of Ste6p, the a-factor transporter of Saccharomyces cerevisiae. Mol. Biol. Cell 5:1185-1198.
6. Berkower, C., and S. Michaelis. 1991. Mutational analysis of the yeast a-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily. EMBO J. 10:3777-3785.
7. Brake, A., C. Brenner, R. Najarian, P. Laybourn, and J. Merriweather. 1985. Structures of genes encoding precursors of the yeast peptide mating pheromone a-factor, p. 103-108. In M.-J. Gething (ed.), Current communications in molecular biology: protein transport and secretion. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
8. Caldwell, G. A., S.-H. Wang, C.-B. Xue, Y. Jiang, H.-F. Lu, F. Naider, and J. M. Becker. 1994 Molecular determinants of bioactivity of the Saccharomyces cerevisiae lipopeptide mating pheromone. J. Biol. Chem. 269:19817-19826.
9. Cheng, S. H., R. J. Gregory, J. Marshall, S. Paul, D. W. Souza, G. A. White, C. R. O'Riordan, and A. E. Smith. 1990. Defective intracellular transport and processing is the molecular basis of most cystic fibrosis. Cell 63:827-534.
10. Cutting, G. R., L. M. Kasch, B. J. Rosenstein, J. Zielenski, L.-C. Tsui, S. E. Antonarakis, and H. H. Kazazian. 1990. A cluster of cystic fibrosis mutations in the first nucleotide binding fold of the cystic fibrosis conductance regulator protein. Nature (London) 346:366-369.
11. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
12. Elia, L., and L. Marsh. 1995. Genes required for cell fusion during mating. p. 21. In Cold Spring Harbor Yeast Cell Biology Meeting Abstracts. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
13. Emr, S. D., A. Vassarotti, J. Garrett, B. L. Geller, M. Takeda, and M. G. Douglas. 1986. The amino terminus of the yeast F1-ATPase β subunit precursor functions as a mitochondrial import signal. J. Cell Biol. 102:523-533.
14. Geitz, R. D., and A. Sugino. 1988. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74:527-534.
15. Gregory, R. J., D. P. Rich, S. H. Cheng, D. W. Souza, S. Paul, P. Manavalan, M. P. Anderson, M. J. Welsh, and A. E. Smith. 1991. Maturation and function of cystic fibrosis transmembrane conductance regulator variants bearing mutations in putative nucleotide-binding domains 1 and 2. Mol Cell. Biol. 11:3886-3893.
16. Harlow, E., and D. Lane. 1988. Antibodies, a laboratory manual. Cold Spring Harbor Press, Cold Spring Harbor, N.Y.
17. Higgins, C. F., S. C. Hyde, M. M. Mimmack, U. Gileadi, D. R. Gill, and M. P. Gallagher. 1990. Binding protein-dependent transport systems. J. Bioenerg. Biomembr. 22:571-592.
18. Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol 153:163-168.
19. Jackson, C. L., and L. H. Hartwell. 1990. Courtship in S. cerevisiae: both cell types choose mating partners by responding to the strongest pheromone signal. Cell 63:1039-1051.
20. Jackson, C. L., and L. H. Hartwell. 1990. Courtship in Saccharomyces cerevisiae: an early cell-cell interaction during mating. Mol. Cell. Biol. 10:2202-2213
21. Jackson, C. L., J. B. Konopka, and L. H. Hartwell. 1991. S cerevisiae α pheromone receptors activate a novel signal transduction pathway for mating partner discrimination. Cell 67:389-402.
22. Julius, D., R. Schekman, and J. Thorner. 1984. Glycosylation and processing of prepro-α-factor through the yeast secretory pathway. Cell 36:309-318.
23. Kerem, B.-S., J. Zielenski, D. Markiewicz, D. Bozon, E. Gazit, J. Yahav, D. Kennedy, J. R. Riordan, F. S. Collins, J. M. Rommens, and L.-C. Tsui. 1990. Identification of mutations in regions corresponding to the two putative nucleotide (ATP) binding folds of the cystic fibrosis gene. Proc. Natl. Acad. Sci. USA 87:8447-8451.
24. Koerner, T. J., J. E. Hill, A. M. Myers, and A. Tzagoloff. 1991. High expression vectors with multiple cloning sites for construction of trpE fusion proteins Methods Enzymol. 194:477-490.
25. Kolling, R., and C. P. Hollenberg. 1994. The ABC transporter Ste6 accumulates in the plasma membrane in a ubiquinated form in endocytosis mutants. EMBO J. 13:3261-3271
26. Kubler, E., and H. Riezman. 1993. Actin and fimbrin are required for the internalization step of endocytosis in yeast. EMBO J. 12:2855-2862.
27. Kuchler, K., H. G. Dohlman, and J. Thorner. 1993. The a-factor transporter (STE6 gene product) and cell polarity in the yeast Saccharomyces cerevisiae. J. Cell Biol. 120:1203-1215.
28. Kuchler, K., R. Sterne, and J. Thorner. 1989 Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells EMBO J. 8:3973-3984.
29. Kunkel, T. A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
30. Kurjan, J. 1992. Pheromone response in yeast. Annu. Rev. Biochem. 61: 1097-1129
31. Marcus, S., G. A. Caldwell, D. Miller, C.-B. Xue, F. Naider, and J. M. Becker. 1991. Significance of C-terminal cysteine modifications to the biological activity of the Saccharomyces cerevisiae a-factor mating pheromone. Mol. Cell. Biol. 11:3603-3612.
32. Marsh, L. 1992. Substitutions in the Hydrophobic core of the α-factor receptor of Saccharomyces cerevisiae permit response to Saccharomyces kluyven α-factor and to antagonist. Mol. Cell. Biol. 12:3959-3966.
33. McGrath, J. P., and A. Varshavsky. 1989. The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein. Nature (London) 340:400-404.
34. Michaelis, S., and I. Herskowitz. 1988. The a-factor pheromone of Saccharomyces cerevisiae is essential for mating. Mol. Cell. Biol. 8:1309-1318.
35. Miller, J. H. 1992. A short course in bacterial genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
36. Mimura, C. S., S. R. Holbrook, and G. F.-L. Ames. 1991. Structural model of the nucleotide-binding conserved component of periplasmic permeases. Proc. Natl. Acad. Sci. USA 88:84-88.
37. Reid, B. J., and L. H. Hartwell. 1977. Regulation of mating in the cell cycle of Saccharomyces cerevisiae. J. Cell Biol. 75:355-365.
38. Rose, M. D., F. Winston, and P. Hieter. 1990. Methods in yeast genetics: a laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
39. Rothstein, R. J. 1983. One-step gene disruption in yeast. Methods Enzymol. 101:202-209.
40. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
41. Saraste, M., P. R. Sibbald, and A. Wittinghofer. 1990. The P-loop - a common motif in ATP- and GTP-binding proteins. Trends Biochem. Sci. 15:430-434.
42. Schafer, W. R., and J. Rine. 1992. Protein prenylation: genes, enzymes, targets, and functions Annu. Rev. Genet. 26:209-237.
43. Sprague, G. 1991. Assay of yeast mating function. Methods Enzymol. 194: 77-93.
44. Sprague, G., and I. Herskowitz. 1981. Control of yeast cell type by the mating type locus identification and control of expression of the a-specific gene BAR1. J. Mol. Biol. 153:305-321.
45. Sprague, G. F., and J. W. Thorner. 1992. Pheromone response and signal transduction during the mating process of Saccharomyces cerevisae, p. 657-744. In E. Jones, J. R. Pringle, and J. R. Broach (ed.), The molecular and cellular biology of the yeast Saccharomyces: gene expression. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
46. Sterne, R. E. 1989. A novel pathway for peptide hormone biogenesis: processing and secretion of the mating pheromone a-factor by Saccharomyces cerevisiae. Ph D. thesis. University of California, Berkeley.
47. Strong, T. V., L. S. Smit, S. V. Turpin, J. L. Cole, C. T. Hon, D. Markiewicz, T. L. Petty, M. W. Craig, E. C. Rosenow III, L.-C. Tsui, M. C. Iannuzzi, M. R. Knowles, and F. S. Collins. 1991 Cystic fibrosis gene mutation in two sisters with mild disease and normal sweat levels. N Engl. J. Med. 325:1630-1634
48. Tsui, L.-C. 1992 Mutations and sequence variations detected in the cystic fibrosis transmembrane conductance regulator (CFTR) gene: a report from the Cystic Fibrosis Genetic Analysis Consortium. Hum. Mutat. 1:197-203.
49. Walker, J. E., M. Saraste, M. J. Runswick, and N. J. Gay. 1982. Distantly related sequences in the α- and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 8:945-951.
50. Wilson, K. L., and I. Herskowitz. 1984. Negative regulation of STE6 gene expression by the α2 product of Saccharomyces cerevisiae. Mol. Cell. Biol. 4:2420-2427.
51. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.