The metabolism of sphingo(glyco)lipids is correlated with the differentiation-dependent autophagic pathway in HT-29 cells

European Journal of Biochemistry

Volumn 237, Issue 2, 1996, Pages 454-459

  Ghidoni, Riccardo ^a,c   Houri, Jean Jacques ^b   Giuliani, Attilia ^a   Ogier Denis, Eric ^b   Parolari, Elena ^a   Botti, Sara ^a   Bauvy, Chantal ^b   Codogno, Patrice ^b  

^a UNIVERSITY OF MILAN   (Italy)

^b INSERM   (France)

^c SAN RAFFAELE HOSPITAL   (Italy)

Author keywords

Autophagy; Glycolipids; HT 29 cells; Lipid traffic; Sphingomyelin

Indexed keywords

ASPARAGINE; COMPLEMENTARY DNA; GANGLIOSIDE GM1; GLYCOLIPID; GLYCOPROTEIN; PERTUSSIS TOXIN; PROTEIN G; SPHINGOSINE;

ARTICLE; AUTOPHAGY; BIODEGRADATION; CELL DIFFERENTIATION; COLON CANCER; CONTROLLED STUDY; GENE EXPRESSION; GENE TRANSFER; GLYCOPROTEIN SYNTHESIS; HUMAN; HUMAN CELL; LIPID METABOLISM; PRIORITY JOURNAL; THIN LAYER CHROMATOGRAPHY; TUMOR CELL LINE;

EID: 0029867412     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0454k.x     Document Type: Article

References (41)

1. van Meer, G. (1989) Lipid traffic in animal cells, Annu. Rev. Cell Biol. 5, 247-275.
2. Hoekstra, D. & Kok, J. W. (1992) Trafficking of glycosphingolipids in eukaryotic cells. Sorting and and recycling of lipids. Biochim. Biophys. Acta 1113, 277-294.
3. van Echten, G. & Sandhoff, K. (1993) Ganglioside metabolism - enzymology, topology, and regulation. J. Biol. Chem. 268, 5341-5344.
4. Mandon, E. C., Ehses, I., Rother, J., van Echten, G. & Sandhoff, K. (1992) Subcellular localization and membrane topology of serine palmitoyltransferase, 3-dehydrosphinganine reductase, and sphinganine N-acyltransferase in mouse liver, J. Biol. Chem. 267, 11144-11148.
5. Collins, R. N. & Warren, G. (1992) Sphingolipids transport in mitotic HeLa cells, J. Biol. Chem. 267, 24906-24911.
6. Moreau, P., Cassagne, C., Keenan, T. W. & Morré, D. J. (1993) Ceramide excluded from cell-free vesicular lipid transfer from endoplasmic reticulum to Golgi apparatus. Evidence for lipid sorting, Biochim. Biophys. Acta 1146, 9-16.
7. Futerman, A. H., Stieger, B., Hubbard, A. L. & Pagano, R. E. (1990) Sphingomyelin synthesis in rat liver occurs predominantly at the cis and medial cisternae of the Golgi apparatus, J. Biol. Chem. 265, 8650-8657.
8. Coste, H., Martel, M. B. & Got, R. (1986) Topology of glucosylceramide synthesis in Golgi membrane from porcine submaxillary glands, Biochim. Biophys. Acta 858, 6-12.
9. Futerman, A. H. & Pagano, R. E. (1991) Determination of the intracellular sites and topology of glucosylceramide synthesis in rat liver, Biochem. J. 280, 295-302.
10. Trinchera, M., Fabbri, M. & Ghidoni, R. (1991) Topography of glycosyltransferases involved in the initial glycosylation of gangliosides, J. Biol. Chem. 266, 20907-20912.
11. Jeckel, J., Karrenbauer, A., Burger, K. N. J., van Meer, G. & Wieland, F. (1992) Glucosylceramide is synthesized at the cytosolic surface of various Golgi subfractions, J. Cell Biol. 117, 259-267.
12. Young, W. W., Lutz, M. S., Mills, S. E. & Osborn-Lechler, S. (1990) Use of brefeldin A to define sites of glycosphingolipid synthesis GA2/GM2/GD2 synthase is trans to the BFA block, Proc. Natl Acad. Sci. USA 87, 6838-6842.
13. Wattenberg, B. W. (1990) Glycolipid and glycoprotein transport through the Golgi complex are similar biochemically and kinetically, Reconstitution of glycolipid transport in a cell free system, J. Cell Biol. 111, 421-428.
14. Young, W. W., Lutz, M. S. & Blackburn, W. A. (1992) Endogenous glycosphingolipids move to the cell surface at a rate consistent with bulk flow estimates. J. Biol. Chem. 267, 12011-12015.
15. Pohlentz, G., Klein, D., Schwarzmann, G., Schmitz, D. & Sandhoff, K. (1988) Both GA2, GM2, and GD2 synthases and GM1b, GD1a and GT1b synthases are single enzymes in Golgi vesicles from rat liver, Proc. Natl Acad. Sci. USA 85, 7044-7048.
16. 2-Asn species is indicative of an impaired N-glycan trimming in undifferentiated cells, J. Biol. Chem. 263, 6031-6037.
17. Trugnan, G., Ogier-Denis, E., Sapin, C., Darmoul, D., Bauvy, C., Aubery, M. & Codogno, P. (1991) The N-glycan processing in HT-29 cells is a function of their state of enterocytic differentiation. Evidence for an atypical traffic associated with change in polypeptide stability in undifferentiated HT-29 cells, J. Biol. Chem. 266, 20849-20855.
18. Houri, J. J., Ogier-Denis, E., Bauvy, C., Aubery, M., Sapin, C., Trugnan, G. & Codogno, P. (1992) Swainsonine is a useful tool to monitor the intracellular traffic of N-linked glycoproteins as a function of the state of enterocytic differentiation of HT-29 cells, Eur. J. Biochem. 205, 1169-1174.
19. Houri, J. J., Ogier-Denis, E., Trugnan, G. & Codogno, P. (1993) Autophagic degradation of N-linked glycoproteins is downregulated in differentiated human colon adenocarcinoma cells, Biochem. Biophys. Res. Commun. 197, 805-811.
20. Houri, J. J., Ogier-Denis, E., De Stefanis, D., Bauvy, C., Baccino, F. M., Isidoro, C. & Codogno, P. (1995) Differentiation-dependent autophagy controls the fate of newly synthesized N-linked glycoproteins in the colon adenocarcinoma HT-29 cell line, Biochem. J. 309, 521-527.
21. Ogier-Denis, E., Couvineau, A., Maoret, J. J., Houri, J. J., Bauvy, C., De Stefanis, D., Isidoro, C., Laburthe, M. & Codogno, P. (1995) A heterotrimeric Gi3-protein controls autophagic sequestration in the human colon cancer cell line HT-29, J. Biol. Chem. 270, 13-16.
22. Houri, J. J., Falbo, A., Vignali, G., Codogno, P. & Ghidoni, R. (1994) The metabolic processing of glycosphingolipids in HT-29 cells is differentiation-dependent, Biochem. Biophys. Res. Commun. 202, 992-1000.
23. Tettamanti, G., Bonali, F., Marchesini, S. & Zambotti, V. (1973) A new procedure for extraction, purification and fractionation of brain gangliosides. Biochim. Biophys. Acta 296, 160-170.
24. Ghidoni, R., Sonnino, S., Masserini, M., Orlando, P. & Tettamanti, G. (1981) Specific tritium labelling of gangliosides at the 3-position of sphingosines, J. Lipid Res. 22, 1286-1295.
25. Sonnino, S., Ghidoni, R., Gazzotti, G., Kirschner, G., Galli, G. & Tettamanti, G. (1984) High performance liquid chromatography preparation of the molecular species of GM1 and GD1a gangliosides with homogeneous long chain base composition, J. Lipid Res. 25, 620-629.
26. Iwamori, M., Moser, H. W. & Kishimoto, Y. (1975) Specific tritium labeling of cerebrosides at the 3-positions of erythro-sphingosine and threo-sphingosine, J. Lipid Res. 16, 332-336.
27. Wice, B. M., Trugnan, G., Pinto, M., Rousset, M., Chevalier, G., Dussaulx, E., Lacroix, B. & Zweibaum, A. (1985) The intracellular accumulation of UDP-N-acetylhexosamines is concomitant with the inability of human colon cancer cells to differentiate, J. Biol. Chem. 260, 139-146.
28. Merrill, A. H. Jr, van Echten, G., Wang, E. & Sandhoff, K. (1993) Fumonisin B1 inhibits sphingosine (sphinganine) N-acyltransferase and de novo sphingolid biosynthesis in cultured neurons in situ, J. Biol. Chem. 268, 27299-27306.
29. Trinchera, M. & Ghidoni, R. (1990) Subcellular biosynthesis and transport of gangliosides formed from exogenous lactosylceramide in rat liver, Biochem. J. 266, 363-369.
30. Høyvik, H., Gordon, P. B., Berg, T. O., Strømhaug, P. E. & Seglen, P. O. (1991) Inhibition of autophagic-lysosomal delivery and autophagic lactolysis by asparagine, J. Cell Biol. 113, 1305-1312.
31. Ghidoni, R., Trinchera, M., Venerando, B., Fiorilli, A., Sonnino, S. & Tettamanti, G. (1986) Incorporation and metabolism of exogenous GM1 ganglioside in rat liver, Biochem. J. 237, 147-155.
32. Tooze, J., Hollinshead, M., Ludwig, T., Howell, K., Hoflack, B. & Kern, H. (1990) In exocrine pancreas, the basolateral endocytic pathway converges wtih the autophagic pathway immediately after the early endosome, J. Cell Biol. 111, 329-345.
33. Gordon, P. B., Høyvik, H. & Seglen, P. O. (1992) Prelysosomal and lysosomal connection between autophagy and endocytosis, Biochem. J. 283, 361-369.
34. Rother, J., van Echten, G., Schwarzmann, G. & Sandhoff, K. (1992) Biosynthesis of sphingolipids: dihydroceramide and not sphinganine is desaturated by cultured cells, Biochem. Biophys. Res. Commun. 189, 14-20.
35. Bohley, P. & Seglen, P. O. (1992) Proteases and proteolysis in the lysosome, Experientia 48, 151-157.
36. Dunn, W. A. Jr (1994) Autophagy and related mechanisms of lysosome-mediated protein degradation, Trends Cell Biol. 4, 139-143.
37. Mortimore, G. E. & Kadowaki, M. (1994) Autophagy: its mechanism and regulation, in Cellular proteolytic systems (Ciechanover, A. J. & Schwartz, A. L., eds) pp. 65-87, Wiley-Liss, New York.
38. Hannun, Y. A. & Bell, R. M. (1989) Functions of sphingolipids and sphingolipid breakdown products in cellular regulation, Science 243, 500-507.
39. Babia, T., Kok, J. W., Hulstaert, C., Deweerd, H. & Hoekstra, D. (1993) Differential metabolism and trafficking of sphingolipids in differentiated versus undifferentiated HT29 cells, Int. J. Cancer 54, 839-845.
40. 6-NBD-glucosylceramide, is subjected to extensive degradation in the plasma membrane: implications for signal transduction related to cell differentiation. Biochem. J. 309, 905-912.
41. Ogier-Denis, E., Trugnan, G., Sapin, C., Aubery, M. & Codogno, P. (1990) Dual effect of 1-deoxymannojirymicin on the mannose uptake and on the N-glycan processing of the human colon cancer cell line HT-29, J. Biol. Chem. 265, 5366-5369.