Measurement of cytotoxicity by propidium iodide staining of target cell DNA application to the quantification of murine TNF-α

Journal of Immunological Methods

Volumn 189, Issue 2, 1996, Pages 243-249

  Wrobel, Kazimierz ^a,b   Claudio, Estefanía ^b   Segade, Fernando ^b   Ramos, Sofía ^b   Lazo, Pedro S ^b  

^a UNIVERSITY OF GUANAJUATO   (Mexico)

^b UNIVERSIDAD DE OVIEDO   (Spain)

Author keywords

Cytotoxicity; Propidium iodide; Tumor necrosis factor

Indexed keywords

PROPIDIUM IODIDE; RECOMBINANT TUMOR NECROSIS FACTOR ALPHA; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ARTICLE; CYTOTOXICITY; MEMBRANE PERMEABILITY; MOUSE; NONHUMAN; PRIORITY JOURNAL;

3T3 CELLS; ANIMALS; CELL MEMBRANE PERMEABILITY; CELL SURVIVAL; DNA; FLUORESCENCE; MICE; PROPIDIUM; STAINING AND LABELING; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0029866782     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(95)00253-7     Document Type: Article

References (22)

1. Beayert, R. and Fiers, W. (1994) Molecular mechanism of tumor necrosis factor a induced cytotoxicity. FEBS Lett. 340, 9-16.
2. Beutler, B. (1992) Tumor Necrosis Factors. The molecules and their emerging role in medicine. Raven Press, New York.
3. Beutler, B. and Cerami, A. (1988) Tumor necrosis, cachexia. shock and inflammation: a common mediator. Ann. Rev. Biochem. 57, 505-518.
4. Claudio, E., Segade, F., Wrobel, K., Ramos, S. and Lazo, P.S. (1995) Activation of murine macrophages by silica particles in vitro can be dissociated from cell toxicity. Am. J. Resp. Cell. Mol. Biol. 13, 547-554.
5. Edidin, M. (1970) A rapid, quantitative fluorescence assay for cell damage by cytotoxic antibodies. J. Immunol. 104, 1303-1306.
6. Espevik, T. and Nissen-Mayer, J. (1986) A high sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. J. Immunol. Methods 95, 99-105.
7. Grell, M., Scheurich, P., Meager, A. and Pfizenmaier, K. (1993) TR60 and TR80 tumor necrosis factor receptors can independently mediate cytolisis. Lymphokine Cytokine Res. 12, 143-148.
8. Heller, R.A., Song, K., Fan, N. and Chang, D.J. (1992) The p70 tumor necrosis factor receptor mediates cytotoxicity. Cell, 70, 47-56.
9. Heller, R.A. and Krönke, M. (1994) Tumor necrosis factor receptor-mediated signaling pathways. J. Cell Biol. 126, 5-9.
10. Higuchi, M. and Aggarwal, B.B. (1992) Microtiter plate radioreceptor assay for tumor necrosis factor and its receptors in large numbers of samples. Anal. Biochem. 204, 53-58.
11. Klefstrom, J., Västrik, I., Saksela, E., Valle, J., Eilers, M. and Alitalo, K. (1994) C-myc induced cellular susceptibility to the cytotoxic action of TNFα. EMBO J. 13, 5442-5450.
12. Kolber, M.A., Quinones, R.R., Gress, R.E. and Henkart, P.A. (1988) Measurement of cytotoxicity by target cell release and retention of the fluorescent dye BCECF. J. Immunol. Methods 108, 255-264.
13. Laster, S.M., Wood, J.G. and Gooding, L.R. (1988) Tumor necrosis factor can induce both apoptotic and necrotic forms of cell lysis. J. Immunol. 141, 2629-2634.
14. Löwik, C.W.G.M., Albblas, M.J., Ruit, M., Papapoulos, S.E. and Pliijm, G. (1993) Quantification of adherent and nonadherent cells cultured in 96-well plates using the supravital stain Neutral Red. Anal. Biochem. 213, 426-433.
15. Nieminen, A.L., Gores, G.J., Bond, J.M., Imberti, R., Herman, B. and Lamasters, J.J. (1992) A novel cytotoxicity screening assay using a multiwell fluorescence scanner. Toxicol. Appl. Pharmacol. 115, 147-155.
16. Sachs, G., Rabon, E. and Karlish, S.J.D. (1990) Transport studies by optical methods. Methods Enzymol. 191, 469-479.
17. Sugarman, B.J., Aggarwal, B.B., Hass, P.E., Figari, I.S., Palladino, Jr. M.A., and Shepard, H.M. (1985). Recombinant human tumor necrosis factor a: effects on proliferation of normal and transformed cells in vitro. Science 230, 943-945.
18. Tartaglia, L.A., Ayres, T.M., Wong, G.H. and Goeddel, D.V. (1993a) A novel domain within the 55 kD TNF receptor signals cell death. Cell 74, 845-853.
19. Tartaglia, L.A., Rome, M., Hu, Y.-F. and Goeddel, D.V. (1993b) Tumor necrosis factor cytotoxic activity is signaled by the p55 TNF receptor. Cell 73, 213-216.
20. Vindelov, L.L., Christensen, I.J. and Nissen, N.I. (1983) A detergent-trypsin method fro the preparation of nuclei for flow cytometry DNA analysis. Cytometry 3, 323-327.
21. 51Cr-labeled targets for natural killer cytotoxicity assay. J. Immunol. Methods 122, 15-24.
22. Wrobel, K., Claudio, E., Segade F., Ramos, S. and Lazo, P.S. (1994) Alteration in membrane permeability is an early event in the cytotoxic effect of TNFα. Biochem. Soc. Trans. 22, 375.