Purification and seroreactivity of pneumococcal surface adhesin A (PsaA)

Clinical and Diagnostic Laboratory Immunology

Volumn 3, Issue 2, 1996, Pages 227-229

  Tharpe, Jean A ^a,b   Russell, Harold ^a  

^a NATIONAL CENTER FOR EMERGING AND ZOONOTIC INFECTIOUS DISEASES   (United States)

^b CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN; BACTERIAL ANTIGEN; PNEUMOCOCCUS VACCINE;

ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; MAJOR CLINICAL STUDY; PNEUMONIA; PRIORITY JOURNAL; STREPTOCOCCUS PNEUMONIAE;

EID: 0029866614     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/cdli.3.2.227-229.1996     Document Type: Article

References (17)

1. Anderson, D. G., and L. L. McKay. 1983. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl. Environ. Microbiol. 46:549-552.
2. Egen, N. B., W. Thormann, G. E. Twitty, and M. Bier. 1984. A new preparative isoelectric focusing apparatus, p. 547-550. In H. Hirai (ed.), Electrophoresis 83. de Gruyter, Berlin.
3. Evans, C. H., A. C. Wilson, and V. A. Gelléri. 1989. Preparative isoelectric focusing in ampholine electrofocusing columns versus immobiline polyacrylamide gel for the purification of biologically active leukoregulin. Anal. Biochem. 177:358-363.
4. Facklam, R. R., and R. F. Breiman. 1991. Current trends in bacterial respiratory pathogens. J. Med. 91(Suppl. 6A):3S-11S.
5. Farrington, M., and D. Rubenstein. 1991. Antigen detection in pneumococcal pneumonia. J. Infect. 23:109-116.
6. Kenna, J. G., G. N. Major, and R. S. Williams. 1985. Methods for reducing non-specific antibody binding in enzyme-linked immunosorbent assays. J. Immunol. Methods 85:409-419.
7. Leinonen, M., H. Syrjälä, E. Jalonen, P. Kujala, and E. Herva. 1990. Demonstration of pneumolysin antibodies in circulating immune complexes - a new diagnostic method for pneumococcal pneumonia. Serodiagn. Immunother. Infect. Dis. 4:451-458.
8. Paton, J. C., R. A. Lock, C. Lee, J. P. Li, A. M. Berry, T. J. Mitchell, P. W. Andrew, D. Hansman, and G. J. Boulnois. 1991. Purification and immuno-genicity of genetically obtained pneumolysin toxoids and their conjugation to Streptococcus pneumoniae type 19F polysaccharide. Infect. Immun. 59:2297-2304.
9. Russell, H., J. A. Tharpe, D. E. Wells, E. H. White, and J. E. Johnson. 1990. Monoclonal antibody recognizing a species-specific protein from Streptococcus pneumoniae. J. Clin. Microbiol. 28:2191-2195.
10. Sampson, J. S., S. P. O'Connor, A. R. Stinson, J. A. Tharpe, and H. Russell. 1994. Cloning and nucleotide sequence analysis of psaA, the Streptococcus pneumoniae gene encoding a 37-kilodalton protein homologous to previously reported Streptococcus sp. adhesins. Infect. Immun. 62:319-324.
11. Sampson, J. S., B. B. Plikaytis, C. H. Aloisio, G. M. Carlone, C. P. Pau, and A. R. Stinson. 1991. Immunologic characterization and specificity of three monoclonal antibodies against the 58-kilodalton protein of Legionella pneumophila. J. Clin. Microbiol. 29:836-841.
12. Siber, G. R. 1994. Pneumococcal disease: prospects for a new generation of vaccines. Science 265:1385-1387.
13. Smith, P. K., R. I. Krohn, G. T. Hermanson, A. D. Mallis, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, N. M. Goeke, B. J. Olson, and D. C. Klenk. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150:76-85.
14. Sorensen, J., I. Cederholm, and C. Carlsson. 1986. Pneumonia: a deadly disease despite intensive care treatment. Scand. J. Infect. Dis. 18:329-335.
15. 1. Protein Expr. Purif. 1:97-103.
16. Veser, J. 1989. Preparative free solution isoelectric focusing of human erythrocyte uroporphyrinogen I synthase in an ampholyte pH gradient. Anal. Biochem. 182:217-221.
17. Williams, W. W., M. A. Hickman, M. A. Kane, A. P. Kendal, J. S. Spika, and A. R. Hinman. 1988. Immunization policies and vaccine coverage among adults: the risk for missed opportunities. Ann. Intern. Med. 108:616-625.