Analysis of CRP-CytR interactions at the Escherichia coli udp promoter

Journal of Bacteriology

Volumn 178, Issue 6, 1996, Pages 1614-1622

  Brikun, Igor ^a,c   Suziedelis, Kestutis ^a,d   Stemmann, Olaf ^a,e   Zhong, Ruigin ^a   Alikhanian, Liubov ^b   Linkova, Elena ^b   Mironov, Alexander ^b   Berg, Douglas E ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b STATE RESEARCH INSTITUTE OF GENETICS AND SELECTION OF INDUSTRIAL MICROORGANISMS   (Russian Federation)

^c ThermoGen Inc   (United States)

^d VILNIUS UNIVERSITY   (Lithuania)

^e UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; CYCLIC AMP BINDING PROTEIN; DEOXYRIBONUCLEASE I; REPRESSOR PROTEIN; URIDINE PHOSPHORYLASE;

ARTICLE; COMPLEX FORMATION; DNA FOOTPRINTING; DNA SEQUENCE; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE INSERTION; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PROTEIN INTERACTION; REGULATOR GENE; TRANSCRIPTION REGULATION;

EID: 0029865234     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.6.1614-1622.1996     Document Type: Article

References (35)

1. Adhya, S. 1989. Multipartite genetic control elements: communication by DNA loop. Annu. Rev. Genet. 23:227-250.
2. Adhya, S. Personal communication
3. Barbier, C. S., and S. A. Short. 1992. Amino acid substitutions in the CytR repressor which alter its capacity to regulate gene expression. J. Bacteriol. 174:2881-2890
4. Brikun, I., K. Suziedelis, and D. E. Berg. 1994. DNA sequence divergence among derivatives of Escherichia coli K-12 detected by PCR-based RAPD DNA fingerprinting. J Bacteriol 176:1673-1682.
5. Chapon, C., and A. Kolb. 1983. Action of CAP on the malT promoter in vitro. J. Bacteriol. 156:1135-1143.
6. Donibrosky, A. J., W. A. Walter, M. T. Record, Jr., D. A. Siegele, and C. A. Gross. 1992. Polypeptides containing highly conserved regions of transcription initiation factor sigma 70 exhibit specificity of binding to promoter DNA. Cell 70:501-512.
7. Galas, D. J., and M. Chandler. 1989. Bacterial insertion sequences, p 109-162. In D. E. Berg and M. M. Howe (ed.), Mobile DNA. American Society for Microbiology, Washington, D C.
8. Galas, D. J., and A. Schmitz. 1978. DNase footprinting: a simple method for detection of protein-DNA binding specificity. Nucleic Acids Res. 5:3157-3170.
9. Gaston, K., A. Bell, A. Kolb, H. Buc, and S. Busby. 1990. Stringent spacing requirements for transcription activation by CRP. Cell 62:733-743.
10. Hammer-Jaspersen, K. 1983. Nucleoside catabolism. Academic Press, London.
11. Hendrickson, W., C. Flaherty, and L. Moltz. 1992. Sequence elements in the Escherichia coli araFGH promoter. J. Bacteriol. 174:6862-6871.
12. Holst, B., L. Sogaard-Andersen, H. Pedersen, and P. Valentin-Hansen. 1992. The cAMP-CRP/CytR nucleoprotein complex in Escherichia coli. two pairs of closely linked binding sites for cAMP-CRP activator complex are involved in combinatorial regulation of the cdd promoter. EMBO J 11:3635-3643.
13. Keilty, S., and M. Rosenberg. 1987 Constitutive function of a positive regulated promoter reveals new sequences essential for activity. J. Biol. Chem. 262:6389-6395.
14. Krishnan, R. B., D. Kersulyte, I. Britain, C. M. Berg, and D. E. Berg. 1991. Direct and crossover PCR amplification to facilitate Tn5supF-based sequencing of lambda phage clones Nucleic Acids Res. 19:6177-6182
15. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
16. Miller, J. H. 1972. Experiments in molecular genetics Cold Spring Harbor Laboratory, Cold Spring Harbor, N Y
17. Mironov, A. S., I. Brikun, A. P. Bolotin, and V. V. Sukhodolets. 1990. Structural and functional arrangement of uridine phosphorylase (udp) gene of Escherichia coli, p. 245-246 In Abstracts of Symposium of Molecular Mechanisms of Genetic Processes Academy of Science of USSR, Moscow.
18. Mironov, A. S., Y. V. Smirnov, and V. V. Sukhodolets. 1975. Further study of the nature of phenotypical reversions in deletion mutants with respect to thymidine phosphorylase of Escherichia coli K-12. Genetika 11:97-105
19. Mulligan, M., and W. McClure. 1986. Analysis of the occurence of promoter sites in DNA Nucleic Acids Res. 14:109-126
20. Neuhard, J., and P. Nigaard. 1987. Purines and pyrimidines, p 445-473. In F. C. Neidhardt, J. L Jngraham, K. B Low, B Magasanik, M Schacchter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
21. O'Neil, M. 1989. Escherichia coli promoters. I. Consensus as it relates to spacing class, specificity, repeat substructure, and three-dimensional organization J. Biol. Chem 264:5522-5530.
22. Pabo, C. O., and R. T. Sauer. 1984. Protein-DNA recognition. Annu. Rev. Biochem. 53:293-321.
23. Pedersen, H., L. Sogaard-Andersen, B. Holst, and P. Valentin-Hansen. 1991. Heterologous cooperativity in Escherichia coli: the CytR repressor both contacts DNA and cAMP receptor protein when binding to the deoP2 promoter. J. Biol. Chem. 266:17804-17808
24. Ponnambalam, S., B. Chan, and S. Busby. 1988 Functional analysis of different sequence elements in the Escherichia coli galactose operon P2 promoter. Mol. Microbiol 2:165-172.
25. Rasmussen, P., L. Sogaard-Andersen, and P. Valentin-Hansen. 1993. Identification of the nucleotide sequence recognized by the cAMP-CRP dependent CytR repressor protein in the deoP2 promoter in E. coli. Nucleic Acids Res. 21:879-885.
26. Rassel, W. E., and H. G. Khorana. 1958. Purification and properties of a pyrimidine deoxynucleoside phosphorylase from Escherichia coli Biochim. Biophys. Acta 28:562-566.
27. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
28. Sogaard-Andersen, L., J. Martinussen, N. E. Mollegaard, S. R. Douthwaite, and P. Valentin-Hansen. 1990 The CytR repressor antagonizes cyclic AMP-cyclic AMP receptor protein activation of the deoCP2 promoter of Escherichia coli K-12. J. Bacteriol. 172:5706-5713.
29. Sogaard-Andersen, L., and P. Valentin-Hansen. 1993. Protein-protein interactions in gene regulation: the cAMP-CRP complex sets the specificity of a second DNA-binding protein, the CytR repressor. Cell 75:557-566
30. Ushida, C., and H. Aiba. 1990. Helical phase-dependent action of CRP; effect of the distance between the CRP site and the -35 region on promoter activity. Nucleic Acids Res. 18:6325-6330.
31. Valentin-Hansen, P. 1982. Tandem CRP sites in the deo operon of Escherichia coli K-12. EMBO J. 1:1049-1054
32. Valentin-Hansen, P., B. Holst, L. Sogaard-Andersen, J. Martinussen, J. Nesvera, and S. R. Douthwaite. 1991. Design of cAMP-CRP activated promoters in Escherichia coli. Mol. Microbiol. 5:433-437.
33. Valentin-Hansen, P., J. E. Love Larsen, P. Hojrup, S. A. Short, and C. S. Barbier. 1986. Nucleotide sequence of the CytR regulatory gene of E. coli K-12. Nucleic Acids Res. 14:2215-2228.
34. Vogel, H. J., and D. M. Bonner. 1956. Acetylornithinase of Escherichia coli: partial purification and some properties. J. Biol. Chem. 218:97-106.
35. Walton, L., C. A. Richards, and L. P. Elwell. 1989. Nucleotide sequence of the Escherichia coli uridine phosphorylase (udp) gene. Nucleic Acids Res. 16:741.