Syntheses of oligonucleotide-amino acid conjugates: Using tentagel and CPG matrices for the synthesis of 3′-phosphoryltyrosine-terminated oligonucleotides

Tetrahedron Letters

Volumn 37, Issue 18, 1996, Pages 3093-3096

  Zhao, Bao Ping ^a   Panigrahi, Gagan B ^a   Sadowski, Paul D ^a   Krepinsky, Jiri J ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGONUCLEOTIDE; PROTEIN DERIVATIVE;

ARTICLE; DNA PROTEIN COMPLEX; DRUG ANALYSIS; DRUG DEVELOPMENT; DRUG SYNTHESIS; REACTION ANALYSIS; TECHNIQUE;

EID: 0029865127     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/0040-4039(96)00369-3     Document Type: Article

References (17)

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10. 1), the conditions not compatible with CPG. Basic conditions lead to the loss of Fmoc protection and a scission of the ester linkage between tyrosine and the support.
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16. 13).
17. 1,2 After incubation with Flp protein the products were analyzed on a denaturing polyacrylamide gel. The oligonucleotides used in A were purified by a reversed phase cartridge while the oligonucleotide used in B was purified by preparative polyacrylamide gel electrophoresis before labelling (A and B depict separate gels).