Crystallization and preliminary diffraction data of a major pollen allergen: Crystal growth separates a low molecular weight form with elevated biological activity

Journal of Biological Chemistry

Volumn 271, Issue 44, 1996, Pages 27193-27196

  Bufe, Albrecht ^a   Betzel, Christian ^b,c   Schramm, Gabriele ^a   Petersen, Arnd ^a   Becker, Wolf Meinhard ^a   Schlaak, Max ^a   Perbandt, Markus ^b   Dauter, Zbigniew ^d   Weber, Wolfgang ^b  

^a RESEARCH CENTER BORSTEL   (Germany)

^b UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^c UNIVERSITY OF HAMBURG   (Germany)

^d EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

POLLEN ANTIGEN; RECOMBINANT PROTEIN; RIBONUCLEASE;

ARTICLE; CRYSTALLIZATION; ENZYME ACTIVITY; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE; X RAY DIFFRACTION;

ALLERGENS; AMINO ACID SEQUENCE; ASTHMA; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; HUMANS; IMMUNOBLOTTING; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PEPTIDE FRAGMENTS; PLANT PROTEINS; POACEAE; POLLEN; RECOMBINANT PROTEINS; RHINITIS, ALLERGIC, SEASONAL; RIBONUCLEASES;

ESCHERICHIA COLI; PHLEUM PRATENSE;

EID: 0029861903     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.271.44.27193     Document Type: Article

References (28)

1. Barnes, P. J. (1991) Trends Biochem. Sci. 16, 365-369
2. King, T. P., Hoffman, D., Løwenstein, H., Marsh, D. G., Platts-Mills, T. A. E., and Thomas, W. (1994) Int. Arch. Allergy Appl. Immunol. 105, 224-233
3. Matthiesen, F., and Løwenstein, H. (1991) Clin. Exp. Allergy 21, 297-307
4. Sutton, B. J., and Gould, H. J. (1993) Nature 366, 421-428
5. Dombrowicz, D., Flamand, V., Brigman, K. K., Koller, B. H., and Kinet, J. P. (1993) Cell 75, 969-976
6. Bufe, A., Becker, W.-M., Schramm, G., Petersen, A., Mamat, U., and Schlaak, M. (1994) J. Allergy Clin. Immunol. 94, 173-181
7. van Milligen, F. J., van't Hof, W., van den Berg, M., and Aalberse, R. C. (1994) J. Allergy Clin. Immunol. 93, 34-43
8. Ball, T., Vrtala, S., Sperr, W. R., Valent, P., Susani, M., Kraft, D., and Valenta, R. (1994) J. Biol. Chem. 269, 28323-28328
9. Ong, E. K., Knox, R. B., and Singh, M. B. (1995) Mol. Immunol. 32, 295-302
10. Schramm, G. (1995) Ph.D. thesis, Universität Bayreuth, Germany
11. Bufe, A., Schramm, G., Keown, M. B., Schlaak, M., and Becker, W.-M. (1995) FEBS Lett. 363, 6-12
12. Petersen, A., Becker, W.-M., and Schlaak, M. (1994) Clin. Exp. Allergy 24, 250-256
13. Becker, W.-M., Schaubschläger, W., Westphal, W., Schlaak, M. (1988) in Biochemical Engineering (Chmiel, H., Hammes, E. P., and Baily, J. E., eds) pp. 440-442, Gustav Fischer Verlag, Stuttgart
14. Hampel, A., Labanaskas, M., Conners, P. G., Kirkegard, L., Raj Bandari, U. L., Sigler, P. B., and Bock, R. M. (1996) Science 162, 1384-1387
15. Otwinowski, Z. (1991) DEN20, A Film Processing Program for Macromolecular Crystallography. Yale University, New Haven, CT
16. Barna, B., Ibenthal, W. D., and Heitefuss, R. (1989) Physiol. Mol. Plant Pathol. 35, 151-160
17. Bufe, A., Spangfort, M. D., Kahlert, H., Schlaak, M., and Becker, W.-M. (1996) Planta 199, 413-415
18. Baur, X., Aschauer, H., Mazur, G., Dewair, M., Prelicz, H., and Steigmann, W. (1989) Science 233, 351-354
19. Topham, C. M., Srinivasan, N., Thorpe, C. J., Overington, P., and Kalsheker, N. A. (1994) Protein Eng. 7, 869-894
20. Metzler, W. J., Valentine, K., Roebber, M., Friedrichs, M. S., Marsh, D. G., and Mueller. L. (1992) Biochemistry 31, 5117-5127
21. Metzler, W. J., Valentine, K., Roebber, M., Marsh, D. G., and Mueller, L. (1992) Biochemistry 31, 8697-8705
22. McPherson, A. (1982) Preparation and Analysis of Protein Crystals, pp. 118-119, John Wiley & Sons, Inc., New York
23. Günther, N., Betzel, C., and Weber, W. (1990) J. Biol. Chem. 265, 2082-2085
24. Fasler, S., Skvaril, F., and Lutz, H. U. (1988) Anal. Biochem. 174, 593-600
25. Hendrickson, W. A., and Wüthrich, K. (1995) Macromolecular Structures, Current Biology Ltd., London
26. Knox, R. B. (1993) Clin. Exp. Allergy 23, 354-359
27. Matousek, J., Trnena, L., Svoboda, P., Oriniakova, P., and Lichtenstein, C. P. (1995) Biol. Chem. Hoppe-Seyler 376, 715-721
28. Johnson, P., and Marsh, D. G. (1965) Nature 206, 935-937