Slit-scan flow cytometry for consistent high resolution DNA analysis of X- and Y-chromosome bearing sperm

Cytometry

Volumn 25, Issue 2, 1996, Pages 191-199

  Rens, Wim ^a   Welch, Glenn R ^a   Houck, David W ^b   Van Oven, Carel H ^c   Johnson, Lawrence A ^a,d  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b BD Technologies   (United States)

^c UNIVERSITY OF AMSTERDAM   (Netherlands)

^d AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

DNA analysis; Gender preselection; Slit scan now cytometry; Sorting; Sperm

Indexed keywords

ARTICLE; CATTLE; CONTROLLED STUDY; DNA CONTENT; FLOW CYTOMETRY; GENDER; MALE; PRIORITY JOURNAL; SPERM; X CHROMOSOME; Y CHROMOSOME;

ANIMALS; CATTLE; CELL SEPARATION; DNA; FLOW CYTOMETRY; MALE; SPERM MOTILITY; SPERM TAIL; SPERMATOZOA; X CHROMOSOME; Y CHROMOSOME;

BOS TAURUS; MAMMALIA;

EID: 0029861675     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19961001)25:2<191::AID-CYTO8>3.0.CO;2-K     Document Type: Article

References (32)

1. Boei JJWA, Balajee AS, Rens W, Aten JA, Mullenders LHF, Natarajan AT: The use of mouse chromosome specific DNA libraries for the detection of X-ray induced aberrations. Int J Radiat Biol 65:583-590, 1994.
2. Boschman GA, Rens W, Manders EMM, Oven CH van, Barendsen GW, Aten JA: On-line sorting of human chromosomes by centromeric index, and identification of sorted populations by GTG- banding and fluorescent in situ hybridization. Hum Genet 85:41-48, 1990.
3. Bakker Schut TC, Florians A, Radosevic K, Grooth BG de, Greve J: Signal processing in slit-scan flow cytometry of cell conjugates. Cytometry 14:459-464, 1993.
4. Benaron DA, Gray JW, Gledhill BL, Lake S, Wyrobek AJ, Young IT: Quantification of mammalian sperm morphology by slit-scan flow cytometry. Cytometry 2:344-349, 1982.
5. Cran DG, Johnson LA, Polge C: Sex preselection in cattle: A field trial. Vet Rec 136:495-496, 1995.
6. Cummins JM and Woodall PF: On mammalian sperm dimensions. J Reprod Fert 75:153-175, 1985.
7. Garner DL, Johnson LA: Viability assessment of mammalian sperm using SYBR-14 and Propidium Iodide. Biol Reprod 53:276-284, 1995.
8. Haugland RP: Molecular Probes. Handbook of fluorescent probes and research chemicals. 1992-1994.
9. Hausmann M, Dudin G, Aten JA, Heilig R, Diaz E, Cremer C: Slit-scan flow cytometry of isolated chromosomes following fluorescence hybridization: An approach of on-line screening for specific chromosomes and chromosome translocations. Z Naturforsch 46c:433-441, 1991.
10. Heilig R, Hausmann M, Rens W, Aten JA, Cremer C: Time optimized analysis of slit-scan chromosome profiles on a general purpose personal computer. Comput Appl Biosci 9:381-385, 1993.
11. Johnson LA, Pinkel D: Modification of a laser-based flow cytometer for high resolution DNA analysis of mammalian spermatozoa. Cytometry 7:268-273, 1986.
12. Johnson LA, Flook JP, Look MV, Pinkel D: Flow sorting of X and Y chromosome- bearing spermatozoa into two populations. Gamete Research 16:1-9, 1987.
13. Johnson LA, Flook JP, Look MV: Flow cytometry of X- and Y-chromosome bearing sperm for DNA using an improved preparation method and staining with Hoechst 33342. Gamete Research 17: 203-212, 1987.
14. Johnson LA, Flook JP, Hawk HW: Sex preselection in rabbits: Live births from X and Y sperm separated by DNA and cell sorting. Biol Reprod 41:199-203, 1989.
15. Johnson LA: Sex preselection in swine: Altered sex ratios in offspring following surgical insemination of flow sorted X- and Y-bearing sperm. Reprod Dornest Anim 26:309-314, 1991.
16. Johnson LA, Welch GR, Keyvanfar K, Dorfmann A, Fugger EF, Schulman JD: Gender preselection in humans? Flow cytometric separation of X and Y spermatozoa for the prevention of X-linked diseases. Hum Reprod 8:1733-1739, 1993.
17. Johnson LA, Welch GR, Garner DL: Improved flow sorting resolution of X- and Y- chromosome bearing viable sperm separation using dual staining and dead cell gating. Cytometry 17(Suppl. 7):83, 1994.
18. Johnson LA: Isolation of X- and Y-bearing sperm for sex preselection. In: Oxford Reviews of Reproductive Biology 16, Charlton H.H. (ed). Oxford University Press, Oxford, UK, 1994, pp 303-326.
19. Johnson LA: Sex preselection by flow cytometric separation of X and Y chromosome-bearing sperm based on DNA difference. Reprod Fertil Dev 7:893-903, 1995.
20. Levinson G, Keyvanfar K, Wu JC, Fugger EF, Fields RA, Harton GL, Palmer FT, Sisson ME, Starr KM, Dennison-Lagos L, Calvo L, Sherins RJ, Bick D, Schulman JD, Black SH: DNA-based X-enriched sperm separation as an adjunct to preimplantation genetic testing for the prevention of X-linked disease. Mol Hum Reprod 1:979-982, 1995.
21. Lucas JN, Gray JW: Centromeric index versus DNA-content flow karyotypes of human chromosomes measured by means of slit-scan flow cytometry. Cytometry 8:273-279, 1987
22. Lucas JN, Mullikan JC, Gray JW: Dicentric chromosome frequency analysis using slit-scan flow cytometry. Cytometry 12:316-322, 1991.
23. Pinkel D, Gledhill BL, Van Dilla MA, Stephenson D, Watchmaker G: High resolution DNA measurements of mammalian sperm. Cytometry 3:1-9, 1982.
24. Rath D, Johnson LA, Dobrinsky JR, Welch GR, Niemann H: Birth of piglets following in vitro fertilization using sperm flow cytometrically sorted for gender. Theriogenology 45(1):256, 1996.
25. Rens W, Van Oven CH, Stap J, Aten JA: Effectiveness of pulse-shape criteria for the selection of dicentric chromosomes by slit-scan flow cytometry and sorting. Anal Cell Pathol 5:147-159, 1993.
26. Rens W, Boschman GA, Hoovers JM, Mandera EM, Slater RM, Stap J, Aten JA: Flow cytometric detection of chromosome abnormalities by measuring centromeric index, DNA content, and DNA base composition. Anal Cell Pathol 6:359-375, 1994.
27. Rens W, Van Oven CH, Stap J, Jakobs ME, Aten JA: Slit-scanning technique using standard cell sorter instruments for analyzing and sorting non-acrocentric human chromosomes, including small ones. Cytometry 16:80-87, 1994.
28. Salisbury GW, VanDemark NL, Lodge JR: Physiology of Reproduction and Artificial Insemination of Cattle, Ed. 4. W.H. Freeman and company, San Francisco, 1978.
29. Steel GD, Torrie JH: Principals and Procedures of Statistics. McGraw-Hill Book Company, Inc., New York, 1960.
30. Van Oven CH, Aten JA: Instruments for real-time pulse- shape analysis of slit-scan flow cytometry signals. Cytometry 11:630-635, 1990.
31. Welch GR, Houck DW, Johnson LA: Fluidic and optical modifications to a FACS IV for flow sorting of X- & Y-chromosome bearing sperm based on DNA. Cytometry 17(Suppl. 7):74, 1994.
32. Wheeless LL, Reeder JE, O-Connel, MJ, Robinson RD, Cosgriff JM, Fradet Y, Frank IN, Cocket ATK: DNA slit-scan flowcytometry of bladder irrigation specimens and the importance of recognizing urothelial cells. Cytometry 12:140-146, 1991.