Conditional circadian dysfunction of the Arabidopsis early-flowering 3 mutant

Science

Volumn 274, Issue 5288, 1996, Pages 790-791

  Hicks, Karen A ^a   Millar, Andrew J ^b,d   Carré, Isabelle A ^b,d   Somers, David E ^b,e   Straume, Martin ^c   Meeks Wagner, D Ry ^a   Kay, Steve A ^b,e  

^a UNIVERSITY OF OREGON   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

^c UNIVERSITY OF VIRGINIA   (United States)

^d UNIVERSITY OF WARWICK   (United Kingdom)

^e SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARABIDOPSIS; ARTICLE; BIOLOGICAL RHYTHM; CIRCADIAN RHYTHM; PHOTOPERIODICITY; PRIORITY JOURNAL; REPRODUCTION; SIGNAL TRANSDUCTION;

ARABIDOPSIS; CIRCADIAN RHYTHM; DARKNESS; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; LIGHT; MOVEMENT; MUTATION; PHENOTYPE; PHOTOPERIOD; PHOTOSYNTHETIC REACTION CENTER COMPLEX PROTEINS; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 0029858646     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5288.790     Document Type: Article

References (20)

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2. P. J. Lumsden, Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 351 (1991); B. Thomas, Flowering Newslett. 16, 6 (1993); G. F. Deitzer. in Manipulation of Flowering, J. G. Atherton, Ed. (Butterworths, London, 1987), pp. 241-253.
3. P. J. Lumsden, Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 351 (1991); B. Thomas, Flowering Newslett. 16, 6 (1993); G. F. Deitzer. in Manipulation of Flowering, J. G. Atherton, Ed. (Butterworths, London, 1987), pp. 241-253.
4. M. Koornneef, C. J. Hanhart, J. H. van der Veen, Mol. Gen. Genet. 229, 57 (1991); K. A. Hicks, A. Sundås, D. R. Meeks-Wagner, Semin. Cell Dev. Biol. 7, 409 (1996).
5. M. Koornneef, C. J. Hanhart, J. H. van der Veen, Mol. Gen. Genet. 229, 57 (1991); K. A. Hicks, A. Sundås, D. R. Meeks-Wagner, Semin. Cell Dev. Biol. 7, 409 (1996).
6. M. T. Zagotta et al., Plant J. 10, 101 (1996).
7. D. F. Wetherell, in The Pulvinus: Motor Organ for Leaf Movement, H. L. G. R. L. Satter and T. C. Vogleman, Eds. (American Society of Plant Physiologists, 1990), pp. 72-78.
8. The KUJATA imaging system recorded cotyledon position every 20 min. Individual traces were analyzed to identify rhythmic behavior and provide a measurement of the period and intensity of rhythmic patterns. Significance levels were taken as 5%. Because the strength of rhythmicity is inversely proportional to relative amplitude, robust rhythmicity was defined as any 19- to 30-hour period with relative amplitude equal to or less than the wild-type mean relative amplitude plus one arithmetic standard deviation. Circadian rhythmicity was defined as robust rhythmicity with a period of 22 to 27 hours. Less than 100% of wild-type seedlings showed robust rhythmicity of leaf movements because of technical difficulties inherent in the assay. Period values are arithmetic means ± SD.
9. A. J. Millar, S. R. Short, N.-H. Chua, S. A. Kay, Plant Cell 4, 1075 (1992).
10. 2 population consisting of a cross between the Columbia and C24 ecotypes was used as a wild-type control.
11. A. J. Millar, I. A. Carré, C. A. Strayer, N.-H. Chua, S. A. Kay, Science 267, 1161 (1995).
12. J. Loros and J. F. Feldman, J. Biol. Rhythms 1, 187 (1986); M. J. Hamblen-Coyle, D. A. Wheeler, J. E. Rutila, M. R. Rosbash, J. C. Hall, J. Insect Behav. 5, 417 (1992); D. A. Wheeler, M. J. Hamblen-Coyle, M. S. Dushay, J. C. Hall, J. Biol. Rhythms 8, 67 (1993).
13. J. Loros and J. F. Feldman, J. Biol. Rhythms 1, 187 (1986); M. J. Hamblen-Coyle, D. A. Wheeler, J. E. Rutila, M. R. Rosbash, J. C. Hall, J. Insect Behav. 5, 417 (1992); D. A. Wheeler, M. J. Hamblen-Coyle, M. S. Dushay, J. C. Hall, J. Biol. Rhythms 8, 67 (1993).
14. J. Loros and J. F. Feldman, J. Biol. Rhythms 1, 187 (1986); M. J. Hamblen-Coyle, D. A. Wheeler, J. E. Rutila, M. R. Rosbash, J. C. Hall, J. Insect Behav. 5, 417 (1992); D. A. Wheeler, M. J. Hamblen-Coyle, M. S. Dushay, J. C. Hall, J. Biol. Rhythms 8, 67 (1993).
15. K. A. Hicks, A. J. Millar, D. E. Somers, D. R. Meeks-Wagner, S. A. Kay, unpublished data.
16. A. J. Millar and S. A. Kay, in preparation.
17. Traces were analyzed as described (5). The mean wild-type period (±SD) was 28.6 ± 0.61 hours on a sample size of six, all of which showed rhythmic behavior. The mean elf3-1 period was 28.8 ± 1.9 hours on a sample size of 18, 16 of which showed rhythmic behavior. The supernova (snv) mutation was isolated in a screen for alterations in cab2-luc expression (13). SNV maps to a locus distinct from the cab2-luc transgene, and the snv mutation has no effect on the period, phase, or relative amplitude of circadian regulation of cab2-luc expression (13). The mean snv period was 25.2 ± 2.3 hours on a sample size of 26, 16 of which showed rhythmic behavior. The mean elf3-1 snv period was 25.3 ± 1.7 hours on a sample size of 40, 21 of which showed rhythmic behavior.
18. D. E. Somers and S. A. Kay, unpublished data.
19. C. Brandes et al., Neuron 16, 687 (1996).
20. We thank K. W. Smith, D. Gerber, and A. Lanham for help in imaging and K. Neale and K. Tracey for technical assistance. K.A.H. thanks members of the Kay laboratory for their hospitality; V. L. Chandler and members of the Chandler laboratory for computer use; J. D. Plautz, J. Hall, and members of the Meeks-Wagner laboratory for useful discussions; and B. A. Bowerman, V. L. Chandler, J. Hollick, E. Kuzminova, and A Schlesinger for critical comments on the manuscript. Supported by grants from the NIH (1R01GM56006) and the NSF Center for Biological Timing (to S.A.K.), and the USDA (93-37100-9189 and 93-37304-9040) and the NSF (MCB-9507218) (to D.R.M.-W.). K.A.H. receives postdoctoral support from NIH and D.E.S. is an NSF Postdoctoral Fellow. S.A.K. is supported by an award from the W. M. Keck Foundation.