Failure to produce transgenic offspring by intra-tubal insemination of gilts with DNA-treated sperm

Reproduction, Fertility and Development

Volumn 8, Issue 7, 1996, Pages 1055-1060

  Gandolfi, F ^a  

^a UNIVERSITY OF MILAN   (Italy)

Author keywords

DNA; Pig; Spermatozoa; Transgenesis

Indexed keywords

DNA; POLYLYSINE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA TRANSFER; FERTILIZATION; GENETIC MANIPULATION; MALE; NONHUMAN; SPERMATOZOON; SWINE; TRANSGENIC ANIMAL; UTERINE TUBE;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BLOTTING, SOUTHERN; CHLORAMPHENICOL O-ACETYLTRANSFERASE; DNA; FEMALE; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; INSEMINATION, ARTIFICIAL; MALE; POLYMERASE CHAIN REACTION; PREGNANCY; PROMOTER REGIONS (GENETICS); SIMIAN VIRUS 40; SPERMATOZOA; SWINE;

ANIMALIA; SUS SCROFA;

EID: 0029856657     PISSN: 10313613     EISSN: None     Source Type: Journal    
DOI: 10.1071/RD9961055     Document Type: Article

References (10)

1. Arezzo, F. (1989). Sea urchin sperm as a vector of foreign genetic information. Cell Biol. Int. Rep. 13, 391-404.
2. Atkinson, P. W., Hines, E. R., Beaton, S., Matthaei, K. I., Reed, K. C., and Bradley, M. P. (1991). Association of exogenous DNA with cattle and insect spermatozoa in vitro. Mol. Reprod. Dev. 29, 1-5.
3. Attal, J., Puissant, C., and Houdebine, L. M. ( 1990). An improvement of a rapid method using Quiagen columns to purify plasmids. Biotechniques 8, 269-71.
4. Brackett, B. G., Baranska, W., Sawichi, W., and Koprowski, H. (1971). Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization. Proc. Natl Acad. Sci. USA 68, 353-7.
5. Brinster, R. L., Chen, H. Y., Trumbauer, M. E., Yagle, M. K., and Palmiter, R. D. (1985). Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc. Natl Acad. Sci. USA 82, 4438-12.
6. Brinster, R. L., Sandgren, E. P., Behringer, R. R., and Palmiter, R. D. (1989). No simple solution for making transgenic mice. Cell 59, 239-41.
7. Camaioni, A., Russo, M. A., Odorisio, T., Gandolfi, F., Fazio, V. M., and Siracusa, G. (1992). Uptake of exogenous DNA by mammalian spermatozoa - specific localization of DNA on sperm heads. J. Reprod. Fertil. 96, 203-12.
8. Castro, F. O., Hernandez, O., Uliver, C., Solano, R., Milanés, C., Aguilar, A., Perez, A., De Armas, R., Herrera, L., and de la Fuente, J. (1990). Introduction of foreign DNA into spermatozoa of farm animals. Theriogenology 34, 1099-110.
9. Courot, M., Bussière, J., Braconnier, O., Forgerit, Y., Després, P., and Martinat-Botté, F. (1995). Insémination intratubaire chez la truie. J. Rech. Porc. (France) 27, 63-6.
10. Xin, S. Y., Dong, C., Qin, G. Z., and Fu, C. Y. (1994). The production of transgenic sheep by sperm mediated gene transfer method. Cell Biol. Int. 18, 464. [Abstr.]